TY - JOUR
T1 - An optical aptasensor for real-time quantification of endotoxin
T2 - From ensemble to single-molecule resolution
AU - Zhu, Pancheng
AU - Papadimitriou, Vasileios
AU - van Dongen, Jeanne E.
AU - Cordeiro, Julia
AU - Neeleman, Yannick
AU - Santoso, Albert
AU - Chen, Shuyi
AU - Eijkel, Jan C.T.
AU - Peng, Hammin
AU - Segerink, Loes
AU - Rwei, Alina
N1 - Funding Information:
This work was funded by Stichting de Weijerhorst, China Scholarship Council (202006830031), Delft University of Technology (A.Y.R. startup fund and Pro2Tech seed award), and Convergence, the alliance between Erasmus Medical Centre Rotterdam, Erasmus University Rotterdam and Delft University of Technology.
Publisher Copyright:
Copyright © 2023 The Authors, some rights reserved.
PY - 2023/2/8
Y1 - 2023/2/8
N2 - Endotoxin is a deadly pyrogen, rendering it crucial to monitor with high accuracy and efficiency. However, current endotoxin detection relies on multistep processes that are labor-intensive, time-consuming, and unsustainable. Here, we report an aptamer-based biosensor for the real-time optical detection of endotoxin. The endotoxin sensor exploits the distance-dependent scattering of gold nanoparticles (AuNPs) coupled to a gold nanofilm. This is enabled by the conformational changes of an endotoxin-specific aptamer upon target binding. The sensor can be used in an ensemble mode and single-particle mode under dark-field illumination. In the ensemble mode, the sensor is coupled with a microspectrometer and exhibits high specificity, reliability (i.e., linear concentration to signal profile in logarithmic scale), and reusability for repeated endotoxin measurements. Individual endotoxins can be detected by monitoring the color of single AuNPs via a color camera, achieving single-molecule resolution. This platform can potentially advance endotoxin detection to safeguard medical, food, and pharmaceutical products.
AB - Endotoxin is a deadly pyrogen, rendering it crucial to monitor with high accuracy and efficiency. However, current endotoxin detection relies on multistep processes that are labor-intensive, time-consuming, and unsustainable. Here, we report an aptamer-based biosensor for the real-time optical detection of endotoxin. The endotoxin sensor exploits the distance-dependent scattering of gold nanoparticles (AuNPs) coupled to a gold nanofilm. This is enabled by the conformational changes of an endotoxin-specific aptamer upon target binding. The sensor can be used in an ensemble mode and single-particle mode under dark-field illumination. In the ensemble mode, the sensor is coupled with a microspectrometer and exhibits high specificity, reliability (i.e., linear concentration to signal profile in logarithmic scale), and reusability for repeated endotoxin measurements. Individual endotoxins can be detected by monitoring the color of single AuNPs via a color camera, achieving single-molecule resolution. This platform can potentially advance endotoxin detection to safeguard medical, food, and pharmaceutical products.
KW - AuNPs
KW - Endotoxins
KW - sing-molecule
U2 - 10.1126/sciadv.adf5509
DO - 10.1126/sciadv.adf5509
M3 - Article
SN - 2375-2548
VL - 9
JO - Science advances
JF - Science advances
IS - 6
M1 - eadf5509
ER -