Analysis of Proteins at the Single Cell Level

    Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

    Abstract

    Proteins bring a consequent additional level of information in comparison with nucleic acids on a cell’s state as proteins are dynamically processed and chemically modified in the cell as a function of the cell life. Yet, the analysis of proteins is challenging as no amplification step is possible as is the case for nucleic acids, and another difficulty lies in the dynamic range of protein expression in a single sample (e.g. a single cell). While the same challenges are still found for microfluidic-based analysis of proteins, microsystems bring about enhanced analytical performance and novel analysis opportunities. This is illustrated here for two different strategies that can be adopted for protein analysis in a chip format. A first strategy consists of transposing the standard proteomic protocol in miniaturized analytical tools, and this provides a number of advantages and enhancement for the analysis: an overall improvement is expected when using smaller systems whose capacity matches better the size of the samples; sample manipulation is minimized when using LOC technology,and this goes together with a decrease in sample loss and contamination; enhanced analytical performance in terms of analysis time and detection sensitivity is ensured by micro- and nano-scale features; last, the use of microfabricated structures guarantees higher analysis reproducibility. In a second strategy, the analysis is actually performed at the single cell level. This strategy does not enable protein mapping anymore, but the investigation focuses on given proteins (a single protein of a small number thereof) which are specifically targeted. For that purpose, innovative microfluidic-based protocols have been developed, and we classify them in three categories of fully destructive, partially invasive and non invasive protocols. Ongoing developments in the area of nanotechnology would enable truly protein mapping at the single cell level, with the use of nanofabricated tools in a LOC platform.
    Original languageUndefined
    Title of host publicationUnravelling Single Cell Genomics: Micro and Nanotools
    EditorsNathalie Bontoux, Luce Dauphinot, Marie-Claude Potier
    Place of PublicationCambridge
    PublisherRoyal Society of Chemistry
    Pages209-242
    Number of pages34
    ISBN (Print)978-1-84755-911-1
    DOIs
    Publication statusPublished - 2010

    Publication series

    NameRSC Nanoscience & Nanotechnology
    PublisherRoyal Society of Chemistry
    Number15
    Volume15
    ISSN (Print)1757-7136

    Keywords

    • METIS-276315
    • EWI-19430
    • IR-75792

    Cite this

    le Gac, S. (2010). Analysis of Proteins at the Single Cell Level. In N. Bontoux, L. Dauphinot, & M-C. Potier (Eds.), Unravelling Single Cell Genomics: Micro and Nanotools (pp. 209-242). (RSC Nanoscience & Nanotechnology; Vol. 15, No. 15). Cambridge: Royal Society of Chemistry. https://doi.org/10.1039/9781849732284-00209