Analyzing shear stress-induced alignment of actin filaments in endothelial cells with a microfluidic assay

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Abstract

The physiology of vascular endothelial cells is strongly affected by fluid shear stress on their surface. In this study, a microfluidic assay was employed to analyze the alignment of actin filaments in endothelial cells in response to shear stress. When cells were cultured in microfluidic channels and subjected to shear stress, the alignment of filaments in the channel direction was significantly higher than in static cultures. By adding inhibitory drugs, the roles of several signaling proteins in the process of alignment were determined. Thus, it is shown how microfluidic technology can be employed to provide a mechanistic insight into cell physiology.
Original languageEnglish
Pages (from-to)11103-
JournalBiomicrofluidics
Volume4
Issue number1
DOIs
Publication statusPublished - 2010

Fingerprint

Endothelial cells
Microfluidics
shear stress
Actins
Shear stress
Assays
filaments
alignment
Physiology
cytology
physiology
cultured cells
drugs
proteins
Proteins
Fluids
fluids
Pharmaceutical Preparations

Keywords

  • METIS-274210
  • IR-74813

Cite this

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abstract = "The physiology of vascular endothelial cells is strongly affected by fluid shear stress on their surface. In this study, a microfluidic assay was employed to analyze the alignment of actin filaments in endothelial cells in response to shear stress. When cells were cultured in microfluidic channels and subjected to shear stress, the alignment of filaments in the channel direction was significantly higher than in static cultures. By adding inhibitory drugs, the roles of several signaling proteins in the process of alignment were determined. Thus, it is shown how microfluidic technology can be employed to provide a mechanistic insight into cell physiology.",
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Analyzing shear stress-induced alignment of actin filaments in endothelial cells with a microfluidic assay. / van der Meer, Andries Dirk; Poot, Andreas A.; Feijen, Jan; Vermes, I.

In: Biomicrofluidics, Vol. 4, No. 1, 2010, p. 11103-.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Analyzing shear stress-induced alignment of actin filaments in endothelial cells with a microfluidic assay

AU - van der Meer, Andries Dirk

AU - Poot, Andreas A.

AU - Feijen, Jan

AU - Vermes, I.

PY - 2010

Y1 - 2010

N2 - The physiology of vascular endothelial cells is strongly affected by fluid shear stress on their surface. In this study, a microfluidic assay was employed to analyze the alignment of actin filaments in endothelial cells in response to shear stress. When cells were cultured in microfluidic channels and subjected to shear stress, the alignment of filaments in the channel direction was significantly higher than in static cultures. By adding inhibitory drugs, the roles of several signaling proteins in the process of alignment were determined. Thus, it is shown how microfluidic technology can be employed to provide a mechanistic insight into cell physiology.

AB - The physiology of vascular endothelial cells is strongly affected by fluid shear stress on their surface. In this study, a microfluidic assay was employed to analyze the alignment of actin filaments in endothelial cells in response to shear stress. When cells were cultured in microfluidic channels and subjected to shear stress, the alignment of filaments in the channel direction was significantly higher than in static cultures. By adding inhibitory drugs, the roles of several signaling proteins in the process of alignment were determined. Thus, it is shown how microfluidic technology can be employed to provide a mechanistic insight into cell physiology.

KW - METIS-274210

KW - IR-74813

U2 - 10.1063/1.3366720

DO - 10.1063/1.3366720

M3 - Article

VL - 4

SP - 11103-

JO - Biomicrofluidics

JF - Biomicrofluidics

SN - 1932-1058

IS - 1

ER -