Angiogenic Factors in Women Ten Years after Severe Very Early Onset Preeclampsia

Ingrid P.M. Gaugler-Senden, Jouke T. Tamsma, Chris van der Bent, Ron Kusters, Eric A.P. Steegers, Christianne J.M. de Groot

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    Abstract

    Background: Women with a history of mainly severe and early onset preeclampsia have an increased risk of future cardiovascular disease. During these complicated pregnancies increased levels of anti-angiogenic factors can be found. We hypothesize that women with a history of severe very early onset preeclampsia still have increased levels of these biomarkers years after this pregnancy, resulting in increased risk for cardiovascular disease.

    Methods: Twenty women with severe early onset preeclampsia before 24 weeks' gestation, who delivered between 1993–2003 in a tertiary referral centre and twenty matched controls with uncomplicated pregnancies and healthy term infants, were addressed for participation in the study. Venous plasma samples were analyzed for basic fibroblast growth factor (bFGF), placental growth factor (PLGF), soluble fms-like tyrosine kinase-1 (sFlt-1), vascular endothelial growth factor (VEGF), E- and P-selectin, soluble intercellular adhesion molecule-3 (sICAM-3) and thrombomodulin by ELISA.

    Results: Sixteen case subjects and 18 control subjects consented participation. The median time interval index pregnancy to study was 9.4 and 9.7 years for cases and controls, respectively. Median levels for cases-controls (p-value) were not different; bFGF: 17.43–11.11 pg/mL (0.33), sFlt-1: 102.98–101.92 pg/ml (0.84), PLGF: 3.57–4.20 pg/mL (0.38), VEGF: 64.05–45.72 pg/mL (0.73), E-selectin: 5.11–4.68 ng/mL (0.20), P-selectin: 85.35–71.69 ng/mL (0.69), sICAM-3: 0.42–0.63 ng/mL (0.41) and Thrombomodulin: 0.92–0.93 ng/mL (0.59).

    Conclusion: There were no differences in angiogenic biomarkers between women with a history of severe early onset preeclampsia versus uncomplicated pregnancy almost 10 years later, suggesting that these angiogenic factors will not contribute to the early detection of women at risk for future cardiovascular disease.
    Original languageEnglish
    Article numbere43637
    Number of pages4
    JournalPLoS ONE
    Volume7
    Issue number8
    DOIs
    Publication statusPublished - 31 Aug 2012

    Cite this

    Gaugler-Senden, I. P. M., Tamsma, J. T., van der Bent, C., Kusters, R., Steegers, E. A. P., & de Groot, C. J. M. (2012). Angiogenic Factors in Women Ten Years after Severe Very Early Onset Preeclampsia. PLoS ONE, 7(8), [e43637]. https://doi.org/10.1371/journal.pone.0043637
    Gaugler-Senden, Ingrid P.M. ; Tamsma, Jouke T. ; van der Bent, Chris ; Kusters, Ron ; Steegers, Eric A.P. ; de Groot, Christianne J.M. / Angiogenic Factors in Women Ten Years after Severe Very Early Onset Preeclampsia. In: PLoS ONE. 2012 ; Vol. 7, No. 8.
    @article{3304ec5d94434ddbab68eb3ed25f2941,
    title = "Angiogenic Factors in Women Ten Years after Severe Very Early Onset Preeclampsia",
    abstract = "Background: Women with a history of mainly severe and early onset preeclampsia have an increased risk of future cardiovascular disease. During these complicated pregnancies increased levels of anti-angiogenic factors can be found. We hypothesize that women with a history of severe very early onset preeclampsia still have increased levels of these biomarkers years after this pregnancy, resulting in increased risk for cardiovascular disease.Methods: Twenty women with severe early onset preeclampsia before 24 weeks' gestation, who delivered between 1993–2003 in a tertiary referral centre and twenty matched controls with uncomplicated pregnancies and healthy term infants, were addressed for participation in the study. Venous plasma samples were analyzed for basic fibroblast growth factor (bFGF), placental growth factor (PLGF), soluble fms-like tyrosine kinase-1 (sFlt-1), vascular endothelial growth factor (VEGF), E- and P-selectin, soluble intercellular adhesion molecule-3 (sICAM-3) and thrombomodulin by ELISA.Results: Sixteen case subjects and 18 control subjects consented participation. The median time interval index pregnancy to study was 9.4 and 9.7 years for cases and controls, respectively. Median levels for cases-controls (p-value) were not different; bFGF: 17.43–11.11 pg/mL (0.33), sFlt-1: 102.98–101.92 pg/ml (0.84), PLGF: 3.57–4.20 pg/mL (0.38), VEGF: 64.05–45.72 pg/mL (0.73), E-selectin: 5.11–4.68 ng/mL (0.20), P-selectin: 85.35–71.69 ng/mL (0.69), sICAM-3: 0.42–0.63 ng/mL (0.41) and Thrombomodulin: 0.92–0.93 ng/mL (0.59).Conclusion: There were no differences in angiogenic biomarkers between women with a history of severe early onset preeclampsia versus uncomplicated pregnancy almost 10 years later, suggesting that these angiogenic factors will not contribute to the early detection of women at risk for future cardiovascular disease.",
    author = "Gaugler-Senden, {Ingrid P.M.} and Tamsma, {Jouke T.} and {van der Bent}, Chris and Ron Kusters and Steegers, {Eric A.P.} and {de Groot}, {Christianne J.M.}",
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    Gaugler-Senden, IPM, Tamsma, JT, van der Bent, C, Kusters, R, Steegers, EAP & de Groot, CJM 2012, 'Angiogenic Factors in Women Ten Years after Severe Very Early Onset Preeclampsia', PLoS ONE, vol. 7, no. 8, e43637. https://doi.org/10.1371/journal.pone.0043637

    Angiogenic Factors in Women Ten Years after Severe Very Early Onset Preeclampsia. / Gaugler-Senden, Ingrid P.M.; Tamsma, Jouke T.; van der Bent, Chris; Kusters, Ron; Steegers, Eric A.P.; de Groot, Christianne J.M.

    In: PLoS ONE, Vol. 7, No. 8, e43637, 31.08.2012.

    Research output: Contribution to journalArticleAcademicpeer-review

    TY - JOUR

    T1 - Angiogenic Factors in Women Ten Years after Severe Very Early Onset Preeclampsia

    AU - Gaugler-Senden, Ingrid P.M.

    AU - Tamsma, Jouke T.

    AU - van der Bent, Chris

    AU - Kusters, Ron

    AU - Steegers, Eric A.P.

    AU - de Groot, Christianne J.M.

    PY - 2012/8/31

    Y1 - 2012/8/31

    N2 - Background: Women with a history of mainly severe and early onset preeclampsia have an increased risk of future cardiovascular disease. During these complicated pregnancies increased levels of anti-angiogenic factors can be found. We hypothesize that women with a history of severe very early onset preeclampsia still have increased levels of these biomarkers years after this pregnancy, resulting in increased risk for cardiovascular disease.Methods: Twenty women with severe early onset preeclampsia before 24 weeks' gestation, who delivered between 1993–2003 in a tertiary referral centre and twenty matched controls with uncomplicated pregnancies and healthy term infants, were addressed for participation in the study. Venous plasma samples were analyzed for basic fibroblast growth factor (bFGF), placental growth factor (PLGF), soluble fms-like tyrosine kinase-1 (sFlt-1), vascular endothelial growth factor (VEGF), E- and P-selectin, soluble intercellular adhesion molecule-3 (sICAM-3) and thrombomodulin by ELISA.Results: Sixteen case subjects and 18 control subjects consented participation. The median time interval index pregnancy to study was 9.4 and 9.7 years for cases and controls, respectively. Median levels for cases-controls (p-value) were not different; bFGF: 17.43–11.11 pg/mL (0.33), sFlt-1: 102.98–101.92 pg/ml (0.84), PLGF: 3.57–4.20 pg/mL (0.38), VEGF: 64.05–45.72 pg/mL (0.73), E-selectin: 5.11–4.68 ng/mL (0.20), P-selectin: 85.35–71.69 ng/mL (0.69), sICAM-3: 0.42–0.63 ng/mL (0.41) and Thrombomodulin: 0.92–0.93 ng/mL (0.59).Conclusion: There were no differences in angiogenic biomarkers between women with a history of severe early onset preeclampsia versus uncomplicated pregnancy almost 10 years later, suggesting that these angiogenic factors will not contribute to the early detection of women at risk for future cardiovascular disease.

    AB - Background: Women with a history of mainly severe and early onset preeclampsia have an increased risk of future cardiovascular disease. During these complicated pregnancies increased levels of anti-angiogenic factors can be found. We hypothesize that women with a history of severe very early onset preeclampsia still have increased levels of these biomarkers years after this pregnancy, resulting in increased risk for cardiovascular disease.Methods: Twenty women with severe early onset preeclampsia before 24 weeks' gestation, who delivered between 1993–2003 in a tertiary referral centre and twenty matched controls with uncomplicated pregnancies and healthy term infants, were addressed for participation in the study. Venous plasma samples were analyzed for basic fibroblast growth factor (bFGF), placental growth factor (PLGF), soluble fms-like tyrosine kinase-1 (sFlt-1), vascular endothelial growth factor (VEGF), E- and P-selectin, soluble intercellular adhesion molecule-3 (sICAM-3) and thrombomodulin by ELISA.Results: Sixteen case subjects and 18 control subjects consented participation. The median time interval index pregnancy to study was 9.4 and 9.7 years for cases and controls, respectively. Median levels for cases-controls (p-value) were not different; bFGF: 17.43–11.11 pg/mL (0.33), sFlt-1: 102.98–101.92 pg/ml (0.84), PLGF: 3.57–4.20 pg/mL (0.38), VEGF: 64.05–45.72 pg/mL (0.73), E-selectin: 5.11–4.68 ng/mL (0.20), P-selectin: 85.35–71.69 ng/mL (0.69), sICAM-3: 0.42–0.63 ng/mL (0.41) and Thrombomodulin: 0.92–0.93 ng/mL (0.59).Conclusion: There were no differences in angiogenic biomarkers between women with a history of severe early onset preeclampsia versus uncomplicated pregnancy almost 10 years later, suggesting that these angiogenic factors will not contribute to the early detection of women at risk for future cardiovascular disease.

    U2 - 10.1371/journal.pone.0043637

    DO - 10.1371/journal.pone.0043637

    M3 - Article

    VL - 7

    JO - PLoS ONE

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    SN - 1932-6203

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    Gaugler-Senden IPM, Tamsma JT, van der Bent C, Kusters R, Steegers EAP, de Groot CJM. Angiogenic Factors in Women Ten Years after Severe Very Early Onset Preeclampsia. PLoS ONE. 2012 Aug 31;7(8). e43637. https://doi.org/10.1371/journal.pone.0043637