Assembled microneedle arrays enhance the transport of compounds varying over a large range of molecular weight across human dermatomed skin

F.J. Verbaan, S.M. Bal, D.J. van den Berg, W.H.H. Groenink, H. Verpoorten, R. Lüttge, J.A. Bouwstra

Research output: Contribution to journalArticleAcademicpeer-review

130 Citations (Scopus)

Abstract

In this study, we demonstrate the feasibility to use microneedle arrays manufactured from commercially available 30G hypodermal needles to enhance the transport of compounds up to a molecular weight of 72 kDa. Piercing of human dermatomed skin with microneedle arrays was studied by Trypan Blue staining on the SC side of the skin and transepidermal water loss measurements (TEWL). Passive transport studies were conducted with Cascade Blue (CB, Mw 538), Dextran–Cascade Blue (DCB, Mw 10 kDa), and FITC coupled Dextran (FITC-Dex, Mw 72 kDa). Microneedle arrays with needle lengths of 900, 700 and 550 μm are able to pierce dermatomed human skin as evident from (a) the appearance of blue spots on the dermal side of the skin after Trypan Blue treatment and (b) elevated TEWL levels after piercing compared to non-treated human dermatomed skin. Microneedles with a length of 300 μm did not pierce human skin in vitro. Transport studies performed with model compounds ranging from 538 Da to 72 kDa revealed that pretreatment with microneedle arrays enhanced the transport across dermatomed human skin. However, some degradation was also observed for FITC-Dex and DCB. We conclude that assembled microneedle arrays can be used to deliver compounds through the skin up to a molecular weight of at least 72 kDa.
Original languageEnglish
Pages (from-to)238-245
Number of pages8
JournalJournal of controlled release
Volume117
Issue number2
DOIs
Publication statusPublished - 2007

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Molecular Weight
Skin
Trypan Blue
Fluorescein-5-isothiocyanate
Needles
Water
Staining and Labeling

Keywords

  • Macromolecules
  • Transdermal drug delivery
  • Skin
  • Microneedles

Cite this

Verbaan, F. J., Bal, S. M., van den Berg, D. J., Groenink, W. H. H., Verpoorten, H., Lüttge, R., & Bouwstra, J. A. (2007). Assembled microneedle arrays enhance the transport of compounds varying over a large range of molecular weight across human dermatomed skin. Journal of controlled release, 117(2), 238-245. https://doi.org/10.1016/j.jconrel.2006.11.009
Verbaan, F.J. ; Bal, S.M. ; van den Berg, D.J. ; Groenink, W.H.H. ; Verpoorten, H. ; Lüttge, R. ; Bouwstra, J.A. / Assembled microneedle arrays enhance the transport of compounds varying over a large range of molecular weight across human dermatomed skin. In: Journal of controlled release. 2007 ; Vol. 117, No. 2. pp. 238-245.
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Assembled microneedle arrays enhance the transport of compounds varying over a large range of molecular weight across human dermatomed skin. / Verbaan, F.J.; Bal, S.M.; van den Berg, D.J.; Groenink, W.H.H.; Verpoorten, H.; Lüttge, R.; Bouwstra, J.A.

In: Journal of controlled release, Vol. 117, No. 2, 2007, p. 238-245.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Assembled microneedle arrays enhance the transport of compounds varying over a large range of molecular weight across human dermatomed skin

AU - Verbaan, F.J.

AU - Bal, S.M.

AU - van den Berg, D.J.

AU - Groenink, W.H.H.

AU - Verpoorten, H.

AU - Lüttge, R.

AU - Bouwstra, J.A.

PY - 2007

Y1 - 2007

N2 - In this study, we demonstrate the feasibility to use microneedle arrays manufactured from commercially available 30G hypodermal needles to enhance the transport of compounds up to a molecular weight of 72 kDa. Piercing of human dermatomed skin with microneedle arrays was studied by Trypan Blue staining on the SC side of the skin and transepidermal water loss measurements (TEWL). Passive transport studies were conducted with Cascade Blue (CB, Mw 538), Dextran–Cascade Blue (DCB, Mw 10 kDa), and FITC coupled Dextran (FITC-Dex, Mw 72 kDa). Microneedle arrays with needle lengths of 900, 700 and 550 μm are able to pierce dermatomed human skin as evident from (a) the appearance of blue spots on the dermal side of the skin after Trypan Blue treatment and (b) elevated TEWL levels after piercing compared to non-treated human dermatomed skin. Microneedles with a length of 300 μm did not pierce human skin in vitro. Transport studies performed with model compounds ranging from 538 Da to 72 kDa revealed that pretreatment with microneedle arrays enhanced the transport across dermatomed human skin. However, some degradation was also observed for FITC-Dex and DCB. We conclude that assembled microneedle arrays can be used to deliver compounds through the skin up to a molecular weight of at least 72 kDa.

AB - In this study, we demonstrate the feasibility to use microneedle arrays manufactured from commercially available 30G hypodermal needles to enhance the transport of compounds up to a molecular weight of 72 kDa. Piercing of human dermatomed skin with microneedle arrays was studied by Trypan Blue staining on the SC side of the skin and transepidermal water loss measurements (TEWL). Passive transport studies were conducted with Cascade Blue (CB, Mw 538), Dextran–Cascade Blue (DCB, Mw 10 kDa), and FITC coupled Dextran (FITC-Dex, Mw 72 kDa). Microneedle arrays with needle lengths of 900, 700 and 550 μm are able to pierce dermatomed human skin as evident from (a) the appearance of blue spots on the dermal side of the skin after Trypan Blue treatment and (b) elevated TEWL levels after piercing compared to non-treated human dermatomed skin. Microneedles with a length of 300 μm did not pierce human skin in vitro. Transport studies performed with model compounds ranging from 538 Da to 72 kDa revealed that pretreatment with microneedle arrays enhanced the transport across dermatomed human skin. However, some degradation was also observed for FITC-Dex and DCB. We conclude that assembled microneedle arrays can be used to deliver compounds through the skin up to a molecular weight of at least 72 kDa.

KW - Macromolecules

KW - Transdermal drug delivery

KW - Skin

KW - Microneedles

U2 - 10.1016/j.jconrel.2006.11.009

DO - 10.1016/j.jconrel.2006.11.009

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JO - Journal of controlled release

JF - Journal of controlled release

SN - 0168-3659

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ER -