Assembly and Mechanical Properties of the Cargo-Free and Cargo Loaded Bacterial Nanocompartment Encapsulin

J. Snijder, O. Kononova, I.M. Barbu, C. Uetrecht, W.F. Rurup, R.J. Burnley, M.S.T. Koay, Jeroen Johannes Lambertus Maria Cornelissen, W.H. Roos, V. Barsegov, G.J.L. Wuite, G.J.L. Wuite, A.J.R. Heck

Research output: Contribution to journalArticleAcademicpeer-review

24 Citations (Scopus)

Abstract

Prokaryotes mostly lack membranous compartments that are typical of eukaryotic cells, but instead, they have various protein-based organelles. These include bacterial microcompartments like the carboxysome and the virus-like nanocompartment encapsulin. Encapsulins have an adaptable mechanism for enzyme packaging, which makes it an attractive platform to carry a foreign protein cargo. Here we investigate the assembly pathways and mechanical properties of the cargo-free and cargo-loaded nanocompartments, using a combination of native mass spectrometry, atomic force microscopy and multiscale computational molecular modeling. We show that encapsulin dimers assemble into rigid single-enzyme bacterial containers. Moreover, we demonstrate that cargo encapsulation has a mechanical impact on the shell. The structural similarity of encapsulins to virus capsids is reflected in their mechanical properties. With these robust mechanical properties encapsulins provide a suitable platform for the development of nanotechnological applications.
Original languageUndefined
Pages (from-to)2522-2529
Number of pages7
JournalBiomacromolecules
Volume17
Issue number8
DOIs
Publication statusPublished - 2016

Keywords

  • IR-103881
  • METIS-320612

Cite this

Snijder, J., Kononova, O., Barbu, I. M., Uetrecht, C., Rurup, W. F., Burnley, R. J., ... Heck, A. J. R. (2016). Assembly and Mechanical Properties of the Cargo-Free and Cargo Loaded Bacterial Nanocompartment Encapsulin. Biomacromolecules, 17(8), 2522-2529. https://doi.org/10.1021/acs.biomac.6b00469
Snijder, J. ; Kononova, O. ; Barbu, I.M. ; Uetrecht, C. ; Rurup, W.F. ; Burnley, R.J. ; Koay, M.S.T. ; Cornelissen, Jeroen Johannes Lambertus Maria ; Roos, W.H. ; Barsegov, V. ; Wuite, G.J.L. ; Wuite, G.J.L. ; Heck, A.J.R. / Assembly and Mechanical Properties of the Cargo-Free and Cargo Loaded Bacterial Nanocompartment Encapsulin. In: Biomacromolecules. 2016 ; Vol. 17, No. 8. pp. 2522-2529.
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title = "Assembly and Mechanical Properties of the Cargo-Free and Cargo Loaded Bacterial Nanocompartment Encapsulin",
abstract = "Prokaryotes mostly lack membranous compartments that are typical of eukaryotic cells, but instead, they have various protein-based organelles. These include bacterial microcompartments like the carboxysome and the virus-like nanocompartment encapsulin. Encapsulins have an adaptable mechanism for enzyme packaging, which makes it an attractive platform to carry a foreign protein cargo. Here we investigate the assembly pathways and mechanical properties of the cargo-free and cargo-loaded nanocompartments, using a combination of native mass spectrometry, atomic force microscopy and multiscale computational molecular modeling. We show that encapsulin dimers assemble into rigid single-enzyme bacterial containers. Moreover, we demonstrate that cargo encapsulation has a mechanical impact on the shell. The structural similarity of encapsulins to virus capsids is reflected in their mechanical properties. With these robust mechanical properties encapsulins provide a suitable platform for the development of nanotechnological applications.",
keywords = "IR-103881, METIS-320612",
author = "J. Snijder and O. Kononova and I.M. Barbu and C. Uetrecht and W.F. Rurup and R.J. Burnley and M.S.T. Koay and Cornelissen, {Jeroen Johannes Lambertus Maria} and W.H. Roos and V. Barsegov and G.J.L. Wuite and G.J.L. Wuite and A.J.R. Heck",
year = "2016",
doi = "10.1021/acs.biomac.6b00469",
language = "Undefined",
volume = "17",
pages = "2522--2529",
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Snijder, J, Kononova, O, Barbu, IM, Uetrecht, C, Rurup, WF, Burnley, RJ, Koay, MST, Cornelissen, JJLM, Roos, WH, Barsegov, V, Wuite, GJL, Wuite, GJL & Heck, AJR 2016, 'Assembly and Mechanical Properties of the Cargo-Free and Cargo Loaded Bacterial Nanocompartment Encapsulin', Biomacromolecules, vol. 17, no. 8, pp. 2522-2529. https://doi.org/10.1021/acs.biomac.6b00469

Assembly and Mechanical Properties of the Cargo-Free and Cargo Loaded Bacterial Nanocompartment Encapsulin. / Snijder, J.; Kononova, O.; Barbu, I.M.; Uetrecht, C.; Rurup, W.F.; Burnley, R.J.; Koay, M.S.T.; Cornelissen, Jeroen Johannes Lambertus Maria; Roos, W.H.; Barsegov, V.; Wuite, G.J.L.; Wuite, G.J.L.; Heck, A.J.R.

In: Biomacromolecules, Vol. 17, No. 8, 2016, p. 2522-2529.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Assembly and Mechanical Properties of the Cargo-Free and Cargo Loaded Bacterial Nanocompartment Encapsulin

AU - Snijder, J.

AU - Kononova, O.

AU - Barbu, I.M.

AU - Uetrecht, C.

AU - Rurup, W.F.

AU - Burnley, R.J.

AU - Koay, M.S.T.

AU - Cornelissen, Jeroen Johannes Lambertus Maria

AU - Roos, W.H.

AU - Barsegov, V.

AU - Wuite, G.J.L.

AU - Wuite, G.J.L.

AU - Heck, A.J.R.

PY - 2016

Y1 - 2016

N2 - Prokaryotes mostly lack membranous compartments that are typical of eukaryotic cells, but instead, they have various protein-based organelles. These include bacterial microcompartments like the carboxysome and the virus-like nanocompartment encapsulin. Encapsulins have an adaptable mechanism for enzyme packaging, which makes it an attractive platform to carry a foreign protein cargo. Here we investigate the assembly pathways and mechanical properties of the cargo-free and cargo-loaded nanocompartments, using a combination of native mass spectrometry, atomic force microscopy and multiscale computational molecular modeling. We show that encapsulin dimers assemble into rigid single-enzyme bacterial containers. Moreover, we demonstrate that cargo encapsulation has a mechanical impact on the shell. The structural similarity of encapsulins to virus capsids is reflected in their mechanical properties. With these robust mechanical properties encapsulins provide a suitable platform for the development of nanotechnological applications.

AB - Prokaryotes mostly lack membranous compartments that are typical of eukaryotic cells, but instead, they have various protein-based organelles. These include bacterial microcompartments like the carboxysome and the virus-like nanocompartment encapsulin. Encapsulins have an adaptable mechanism for enzyme packaging, which makes it an attractive platform to carry a foreign protein cargo. Here we investigate the assembly pathways and mechanical properties of the cargo-free and cargo-loaded nanocompartments, using a combination of native mass spectrometry, atomic force microscopy and multiscale computational molecular modeling. We show that encapsulin dimers assemble into rigid single-enzyme bacterial containers. Moreover, we demonstrate that cargo encapsulation has a mechanical impact on the shell. The structural similarity of encapsulins to virus capsids is reflected in their mechanical properties. With these robust mechanical properties encapsulins provide a suitable platform for the development of nanotechnological applications.

KW - IR-103881

KW - METIS-320612

U2 - 10.1021/acs.biomac.6b00469

DO - 10.1021/acs.biomac.6b00469

M3 - Article

VL - 17

SP - 2522

EP - 2529

JO - Biomacromolecules

JF - Biomacromolecules

SN - 1525-7797

IS - 8

ER -