Assessing barrier properties using impedance spectroscopy in a semi-circular, blood-brain barrier on-chip

Fotios Avgidis, Martijn Tibbe, Anne Leferink, Loes Segerink*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

Abstract

The blood-brain barrier (BBB) is a fundamental biological structure that isolates the central nervous system from the rest of the body. We have developed a microfluidic BBB model that integrates electrodes, allowing for label-free assessment of the barrier's integrity. Unlike previous efforts, our model reflects the actual size (radius < 30 µm) and circular shape of a human brain microvessel, and achieves membrane-free separation of the basolateral and apical compartments. Using our device, we demonstrated that vitamin C contributes towards a tighter endothelium, and we transiently modulated the tightness of the cell layer by locally depleting calcium ions.

Original languageEnglish
Title of host publication23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
PublisherThe Chemical and Biological Microsystems Society
Pages422-423
Number of pages2
ISBN (Electronic)9781733419000
Publication statusPublished - 2019

Publication series

Name23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019

Keywords

  • Blood-Brain Barrier (BBB)
  • Impedance Spectroscopy
  • Microfluidics
  • Organ-on-a-chip

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