Abstract
The blood-brain barrier (BBB) is a fundamental biological structure that isolates the central nervous system from the rest of the body. We have developed a microfluidic BBB model that integrates electrodes, allowing for label-free assessment of the barrier's integrity. Unlike previous efforts, our model reflects the actual size (radius < 30 µm) and circular shape of a human brain microvessel, and achieves membrane-free separation of the basolateral and apical compartments. Using our device, we demonstrated that vitamin C contributes towards a tighter endothelium, and we transiently modulated the tightness of the cell layer by locally depleting calcium ions.
| Original language | English |
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| Title of host publication | 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 |
| Publisher | The Chemical and Biological Microsystems Society |
| Pages | 422-423 |
| Number of pages | 2 |
| ISBN (Electronic) | 9781733419000 |
| Publication status | Published - 2019 |
| Event | 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2019 - Congress Center Basel, Basel, Switzerland Duration: 27 Oct 2019 → 31 Oct 2019 Conference number: 23 https://cbmsociety.org/microtas/microtas2019/ |
Publication series
| Name | 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 |
|---|
Conference
| Conference | 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2019 |
|---|---|
| Abbreviated title | MicroTAS 2019 |
| Country/Territory | Switzerland |
| City | Basel |
| Period | 27/10/19 → 31/10/19 |
| Internet address |
Keywords
- Blood-Brain Barrier (BBB)
- Impedance Spectroscopy
- Microfluidics
- Organ-on-a-chip