TY - JOUR
T1 - Atomic force microscopy combined with confocal laser scanning microscopy
T2 - a new look at cells
AU - Putman, Constant A.J.
AU - van Leeuwen, Anne Marie
AU - de Grooth, Bart G.
AU - Radosevic, Katarina
AU - van der Werf, Kees O.
AU - van Hulst, Niek F.
AU - Greve, Jan
PY - 1993
Y1 - 1993
N2 - A stand-alone atomic force microscope (AFM) has been developed, which features a large scan area and which allows operation under liquid. This system was combined with a confocal laser scanning microscope (CLSM). Information about cell structures, obtained by CLSM, can be complemented with images of the cell surface obtained with the AFM. This is illustrated by studying the pseudopodia of cells from a human cell line (K562-cells, predecessor of erythroblasts) and the cytoskeleton of monkey kidney cells (in air and under liquid), both stained with F-actin-specific fluorescent probes. Images of the cytoskeleton during the cytotoxic interaction between a natural killer and a K562 target cell are presented. Our results show that combination of these techniques can provide new information about cells and cellular structures.
AB - A stand-alone atomic force microscope (AFM) has been developed, which features a large scan area and which allows operation under liquid. This system was combined with a confocal laser scanning microscope (CLSM). Information about cell structures, obtained by CLSM, can be complemented with images of the cell surface obtained with the AFM. This is illustrated by studying the pseudopodia of cells from a human cell line (K562-cells, predecessor of erythroblasts) and the cytoskeleton of monkey kidney cells (in air and under liquid), both stained with F-actin-specific fluorescent probes. Images of the cytoskeleton during the cytotoxic interaction between a natural killer and a K562 target cell are presented. Our results show that combination of these techniques can provide new information about cells and cellular structures.
KW - Atomic Force Microscopy
KW - Confocal laser scanning microscopy
KW - Cytoskeleton
KW - Cytotoxic interaction
U2 - 10.1002/1361-6374(199306)1:2<63::AID-BIO1>3.0.CO;2-Y
DO - 10.1002/1361-6374(199306)1:2<63::AID-BIO1>3.0.CO;2-Y
M3 - Article
SN - 0966-9051
VL - 1
SP - 63
EP - 70
JO - Bioimaging
JF - Bioimaging
IS - 2
ER -