Atomic force microscopy combined with confocal laser scanning microscopy: a new look at cells

Constant A.J. Putman, Anne Marie van Leeuwen, Bart G. de Grooth, Katarina Radosevic, Kees O. van der Werf, Niek F. van Hulst, Jan Greve

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Abstract

A stand-alone atomic force microscope (AFM) has been developed, which features a large scan area and which allows operation under liquid. This system was combined with a confocal laser scanning microscope (CLSM). Information about cell structures, obtained by CLSM, can be complemented with images of the cell surface obtained with the AFM. This is illustrated by studying the pseudopodia of cells from a human cell line (K562-cells, predecessor of erythroblasts) and the cytoskeleton of monkey kidney cells (in air and under liquid), both stained with F-actin-specific fluorescent probes. Images of the cytoskeleton during the cytotoxic interaction between a natural killer and a K562 target cell are presented. Our results show that combination of these techniques can provide new information about cells and cellular structures.
Original languageEnglish
Pages (from-to)63-70
Number of pages8
JournalBioimaging
Volume1
Issue number2
DOIs
Publication statusPublished - 1993

Keywords

  • Atomic Force Microscopy
  • Confocal laser scanning microscopy
  • Cytoskeleton
  • Cytotoxic interaction

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    Putman, C. A. J., van Leeuwen, A. M., de Grooth, B. G., Radosevic, K., van der Werf, K. O., van Hulst, N. F., & Greve, J. (1993). Atomic force microscopy combined with confocal laser scanning microscopy: a new look at cells. Bioimaging, 1(2), 63-70. https://doi.org/10.1002/1361-6374(199306)1:2<63::AID-BIO1>3.0.CO;2-Y