Au Nanoparticle-Based Amplified DNA Detection on Poly-l-lysine Monolayer-Functionalized Electrodes

Almudena Marti; Jurriaan Huskens

doi:10.3390/nano12020242

Au Nanoparticle-Based Amplified DNA Detection on Poly-l-lysine Monolayer-Functionalized Electrodes

Almudena Marti
, Jurriaan Huskens^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

10 Citations (Scopus)

177 Downloads (Pure)

Abstract

Affinity sensing of nucleic acids is among the most investigated areas in biosensing due to the growing importance of DNA diagnostics in healthcare research and clinical applications. Here, we report a simple electrochemical DNA detection layer, based on poly-l-lysine (PLL), in combination with gold nanoparticles (AuNPs) as a signal amplifier. The layer shows excellent reduction of non-specific binding and thereby high contrast between amplified and non-amplified signals with functionalized AuNPs; the relative change in current was 10-fold compared to the non-amplified signal. The present work may provide a general method for the detection of tumor markers based on electrochemical DNA sensing.

Original language	English
Article number	242
Journal	Nanomaterials
Volume	12
Issue number	2
Early online date	13 Jan 2022
DOIs	https://doi.org/10.3390/nano12020242
Publication status	Published - Jan 2022

Keywords

AuNPs
DNA sensing
Electrochemical biosensor
Poly-l-lysine
Signal amplification

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10.3390/nano12020242Licence: CC BY

nanomaterials-12-00242Final published version, 2.01 MBLicence: CC BY

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title = "Au Nanoparticle-Based Amplified DNA Detection on Poly-l-lysine Monolayer-Functionalized Electrodes",

abstract = "Affinity sensing of nucleic acids is among the most investigated areas in biosensing due to the growing importance of DNA diagnostics in healthcare research and clinical applications. Here, we report a simple electrochemical DNA detection layer, based on poly-l-lysine (PLL), in combination with gold nanoparticles (AuNPs) as a signal amplifier. The layer shows excellent reduction of non-specific binding and thereby high contrast between amplified and non-amplified signals with functionalized AuNPs; the relative change in current was 10-fold compared to the non-amplified signal. The present work may provide a general method for the detection of tumor markers based on electrochemical DNA sensing.",

keywords = "AuNPs, DNA sensing, Electrochemical biosensor, Poly-l-lysine, Signal amplification",

author = "Almudena Marti and Jurriaan Huskens",

note = "Funding Information: Funding: This research was funded by the MESA+ Institute, University of Twente, grant {\textquotedblleft}Early cancer diagnostics{\textquotedblright}. Publisher Copyright: {\textcopyright} 2022 by the authors. Licensee MDPI, Basel, Switzerland. Financial transaction number: 342164094 ",

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doi = "10.3390/nano12020242",

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T1 - Au Nanoparticle-Based Amplified DNA Detection on Poly-l-lysine Monolayer-Functionalized Electrodes

AU - Marti, Almudena

AU - Huskens, Jurriaan

N1 - Funding Information: Funding: This research was funded by the MESA+ Institute, University of Twente, grant “Early cancer diagnostics”. Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland. Financial transaction number: 342164094

PY - 2022/1

Y1 - 2022/1

N2 - Affinity sensing of nucleic acids is among the most investigated areas in biosensing due to the growing importance of DNA diagnostics in healthcare research and clinical applications. Here, we report a simple electrochemical DNA detection layer, based on poly-l-lysine (PLL), in combination with gold nanoparticles (AuNPs) as a signal amplifier. The layer shows excellent reduction of non-specific binding and thereby high contrast between amplified and non-amplified signals with functionalized AuNPs; the relative change in current was 10-fold compared to the non-amplified signal. The present work may provide a general method for the detection of tumor markers based on electrochemical DNA sensing.

AB - Affinity sensing of nucleic acids is among the most investigated areas in biosensing due to the growing importance of DNA diagnostics in healthcare research and clinical applications. Here, we report a simple electrochemical DNA detection layer, based on poly-l-lysine (PLL), in combination with gold nanoparticles (AuNPs) as a signal amplifier. The layer shows excellent reduction of non-specific binding and thereby high contrast between amplified and non-amplified signals with functionalized AuNPs; the relative change in current was 10-fold compared to the non-amplified signal. The present work may provide a general method for the detection of tumor markers based on electrochemical DNA sensing.

KW - AuNPs

KW - DNA sensing

KW - Electrochemical biosensor

KW - Poly-l-lysine

KW - Signal amplification

UR - https://www.scopus.com/pages/publications/85122859618

U2 - 10.3390/nano12020242

DO - 10.3390/nano12020242

M3 - Article

AN - SCOPUS:85122859618

SN - 2079-4991

VL - 12

JO - Nanomaterials

JF - Nanomaterials

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M1 - 242

ER -

Au Nanoparticle-Based Amplified DNA Detection on Poly-l-lysine Monolayer-Functionalized Electrodes

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