TY - JOUR
T1 - Automated 3D Sampling and Imaging of Uneven Sample Surfaces with LA-REIMS
AU - Nauta, Sylvia P.
AU - Huysmans, Pascal
AU - Tuijthof, Gabriëlle J.M.
AU - Eijkel, Gert B.
AU - Poeze, Martijn
AU - Siegel, Tiffany Porta
AU - Heeren, Ron M.A.
N1 - Funding Information:
This research was part of the M4I research program and received financial support from the Dutch Province of Limburg under the LINK program. The research was financially supported by MUMC + (funder name: Maastricht Universitair Medisch Centrum, funder id: 10.13039/501100004528 , Grant No. 15.0869).
Funding Information:
Special thanks to Vincent Franssen, Sander Gerards, Paul Laeven, and Simon Biermans (IDEE, Maastricht University) for their support in the development of the 3D MS scanner. We thank Pieter Emans, Andy Cremers, and Tim Welting (MUMC+) for providing the postoperative human femoral heads, Chris Arts (MUMC+) for his support in developing a sample holder suitable for the analysis of femoral heads, Pieter Kooijman and Benjamin Bartels (M4i) for their support with the registration of the analog laser trigger with the mass spectrometry measurements, and Ian Anthony (M4i) for proofreading the manuscript. We appreciate Waters Corp. (Manchester, UK) for their technical support with the REIMS-XEVO system.
Publisher Copyright:
© 2021 The Authors. Published by American Chemical Society
PY - 2022/1/5
Y1 - 2022/1/5
N2 - The analysis of samples with large height variations remains a challenge for mass spectrometry imaging (MSI), despite many technological advantages. Ambient sampling and ionization MS techniques allow for the molecular analysis of sample surfaces with height variations, but most techniques lack MSI capabilities. We developed a 3D MS scanner for the automated sampling and imaging of a 3D surface with laser-assisted rapid evaporative ionization mass spectrometry (LA-REIMS). The sample is moved automatically with a constant distance between the laser probe and sample surface in the 3D MS Scanner. The topography of the surface was scanned with a laser point distance sensor to define the MS measurement points. MS acquisition was performed with LA-REIMS using a surgical CO2 laser coupled to a qTOF instrument. The topographical scan and MS acquisition can be completed within 1 h using the 3D MS scanner for 300 measurement points on uneven samples with a spatial resolution of 2 mm in the top view, corresponding to 22.04 cm2. Comparison between the automated acquisition with the 3D MS scanner and manual acquisition by hand showed that the automation resulted in increased reproducibility between the measurement points. 3D visualizations of molecular distributions related to structural differences were shown for an apple, a marrowbone, and a human femoral head to demonstrate the imaging feasibility of the system. The developed 3D MS scanner allows for the automated sampling of surfaces with uneven topographies with LA-REIMS, which can be used for the 3D visualization of molecular distributions of these surfaces.
AB - The analysis of samples with large height variations remains a challenge for mass spectrometry imaging (MSI), despite many technological advantages. Ambient sampling and ionization MS techniques allow for the molecular analysis of sample surfaces with height variations, but most techniques lack MSI capabilities. We developed a 3D MS scanner for the automated sampling and imaging of a 3D surface with laser-assisted rapid evaporative ionization mass spectrometry (LA-REIMS). The sample is moved automatically with a constant distance between the laser probe and sample surface in the 3D MS Scanner. The topography of the surface was scanned with a laser point distance sensor to define the MS measurement points. MS acquisition was performed with LA-REIMS using a surgical CO2 laser coupled to a qTOF instrument. The topographical scan and MS acquisition can be completed within 1 h using the 3D MS scanner for 300 measurement points on uneven samples with a spatial resolution of 2 mm in the top view, corresponding to 22.04 cm2. Comparison between the automated acquisition with the 3D MS scanner and manual acquisition by hand showed that the automation resulted in increased reproducibility between the measurement points. 3D visualizations of molecular distributions related to structural differences were shown for an apple, a marrowbone, and a human femoral head to demonstrate the imaging feasibility of the system. The developed 3D MS scanner allows for the automated sampling of surfaces with uneven topographies with LA-REIMS, which can be used for the 3D visualization of molecular distributions of these surfaces.
KW - 3D mass spectrometry imaging
KW - automation
KW - LA-REIMS
KW - surface sampling
KW - n/a OA procedure
UR - http://www.scopus.com/inward/record.url?scp=85121104223&partnerID=8YFLogxK
U2 - 10.1021/jasms.1c00290
DO - 10.1021/jasms.1c00290
M3 - Article
C2 - 34882413
AN - SCOPUS:85121104223
VL - 33
SP - 111
EP - 122
JO - Journal of the American Society for Mass Spectrometry
JF - Journal of the American Society for Mass Spectrometry
SN - 1044-0305
IS - 1
ER -