Automated medium recirculation using macro valves for high flow rates in an endothelial cell culture chip

Elsbeth G.B.M. Bossink; Anke Ricarda Vollertsen; Loes Segerink; A. van der Meer; Mathieu Odijk

Automated medium recirculation using macro valves for high flow rates in an endothelial cell culture chip

Elsbeth G.B.M. Bossink, Anke Ricarda Vollertsen, Loes Segerink, A. van der Meer, Mathieu Odijk

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

1 Citation (Scopus)

106 Downloads (Pure)

Abstract

The vascular endothelium serves an important function in many signaling processes and throughout all of our organs. Accurately mimicking its dynamic environment in vitro requires a wide range of flow control. We present a microfluidic chip fabricated from rapid prototyped molds with which we can automate medium refreshment and medium recirculation using an integrated peristaltic pump. This macro valve-based pump can reach flow rates up to ~30 μL/min and over 3 × 106 valve actuations. Finally, we show that endothelial cells can be cultured in the device for 96 hours under peristaltic flow and with automated medium refreshment.

Original language	English
Title of host publication	25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Subtitle of host publication	10-14 October 2021, Palm Springs, CA, USA
Pages	1269-1270
Number of pages	2
Publication status	Published - 11 Oct 2021
Event	25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2021 - Palm Springs, United States Duration: 10 Oct 2021 → 14 Oct 2021 Conference number: 25 https://microtas2021.org/

Conference

Conference	25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2021
Abbreviated title	MicroTAS 2021
Country/Territory	United States
City	Palm Springs
Period	10/10/21 → 14/10/21
Internet address	https://microtas2021.org/

Keywords

Peristaltic pump
Micromilling
Macro valve
Recirculation
Endothelial cell

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@inproceedings{67d3bff12fd845e884568fe942868af5,

title = "Automated medium recirculation using macro valves for high flow rates in an endothelial cell culture chip",

abstract = "The vascular endothelium serves an important function in many signaling processes and throughout all of our organs. Accurately mimicking its dynamic environment in vitro requires a wide range of flow control. We present a microfluidic chip fabricated from rapid prototyped molds with which we can automate medium refreshment and medium recirculation using an integrated peristaltic pump. This macro valve-based pump can reach flow rates up to ~30 μL/min and over 3 × 106 valve actuations. Finally, we show that endothelial cells can be cultured in the device for 96 hours under peristaltic flow and with automated medium refreshment.",

keywords = "Peristaltic pump, Micromilling, Macro valve, Recirculation, Endothelial cell",

author = "Bossink, {Elsbeth G.B.M.} and Vollertsen, {Anke Ricarda} and Loes Segerink and {van der Meer}, A. and Mathieu Odijk",

year = "2021",

month = oct,

day = "11",

language = "English",

isbn = "978-1-7334190-3-1",

pages = "1269--1270",

booktitle = "25th International Conference on Miniaturized Systems for Chemistry and Life Sciences",

note = "25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2021, MicroTAS 2021 ; Conference date: 10-10-2021 Through 14-10-2021",

url = "https://microtas2021.org/",

}

Bossink, EGBM, Vollertsen, AR , Segerink, L , van der Meer, A & Odijk, M 2021, Automated medium recirculation using macro valves for high flow rates in an endothelial cell culture chip. in 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences: 10-14 October 2021, Palm Springs, CA, USA. pp. 1269-1270, 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2021, Palm Springs, California, United States, 10/10/21.

Automated medium recirculation using macro valves for high flow rates in an endothelial cell culture chip. / Bossink, Elsbeth G.B.M.; Vollertsen, Anke Ricarda ; Segerink, Loes et al.
25th International Conference on Miniaturized Systems for Chemistry and Life Sciences: 10-14 October 2021, Palm Springs, CA, USA. 2021. p. 1269-1270.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

TY - GEN

T1 - Automated medium recirculation using macro valves for high flow rates in an endothelial cell culture chip

AU - Bossink, Elsbeth G.B.M.

AU - Vollertsen, Anke Ricarda

AU - Segerink, Loes

AU - van der Meer, A.

AU - Odijk, Mathieu

N1 - Conference code: 25

PY - 2021/10/11

Y1 - 2021/10/11

N2 - The vascular endothelium serves an important function in many signaling processes and throughout all of our organs. Accurately mimicking its dynamic environment in vitro requires a wide range of flow control. We present a microfluidic chip fabricated from rapid prototyped molds with which we can automate medium refreshment and medium recirculation using an integrated peristaltic pump. This macro valve-based pump can reach flow rates up to ~30 μL/min and over 3 × 106 valve actuations. Finally, we show that endothelial cells can be cultured in the device for 96 hours under peristaltic flow and with automated medium refreshment.

AB - The vascular endothelium serves an important function in many signaling processes and throughout all of our organs. Accurately mimicking its dynamic environment in vitro requires a wide range of flow control. We present a microfluidic chip fabricated from rapid prototyped molds with which we can automate medium refreshment and medium recirculation using an integrated peristaltic pump. This macro valve-based pump can reach flow rates up to ~30 μL/min and over 3 × 106 valve actuations. Finally, we show that endothelial cells can be cultured in the device for 96 hours under peristaltic flow and with automated medium refreshment.

KW - Peristaltic pump

KW - Micromilling

KW - Macro valve

KW - Recirculation

KW - Endothelial cell

M3 - Conference contribution

SN - 978-1-7334190-3-1

SP - 1269

EP - 1270

BT - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences

T2 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2021

Y2 - 10 October 2021 through 14 October 2021

ER -

Automated medium recirculation using macro valves for high flow rates in an endothelial cell culture chip

Abstract

Conference

Keywords

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