AUTOMATED MEDIUM RECIRCULATION USING MACRO VALVES FOR HIGH FLOW RATES IN AN ENDOTHELIAL CELL CULTURE CHIP

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

Abstract

The vascular endothelium serves an important function in many signaling processes and throughout all of our organs. Accurately mimicking its dynamic environment in vitro requires a wide range of flow control. We present a microfluidic chip fabricated from rapid prototyped molds with which we can automate medium refreshment and medium recirculation using an integrated peristaltic pump. This macro valve-based pump can reach flow rates up to ~30 μL/min and over 3 × 106 valve actuations. Finally, we show that endothelial cells can be cultured in the device for 96 hours under peristaltic flow and with automated medium refreshment.
Original languageEnglish
Title of host publication25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Subtitle of host publication10 - 14 October 2021, Palm Springs, CA, USA
Pages1269-1270
Number of pages2
Publication statusPublished - 11 Oct 2021
Event25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2021 - Palm Springs, United States
Duration: 10 Oct 202114 Oct 2021
Conference number: 25
https://microtas2021.org/

Conference

Conference25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2021
Abbreviated titleMicroTAS 2021
CountryUnited States
CityPalm Springs
Period10/10/2114/10/21
Internet address

Keywords

  • Peristaltic pump
  • micromilling
  • macro valve
  • recirculation
  • endothelial cells

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