Axial Resolution of Confocal Raman Microscopes: Gaussian Beam Theory and Practice

C.J. de Grauw, N.M. Sijtsema, C. Otto, J. Greve

Research output: Contribution to journalArticleAcademicpeer-review

39 Citations (Scopus)
206 Downloads (Pure)

Abstract

A straightforward and transparent model, based on Gaussian beam optics, for the axial r0 resolution of a confocal microscope is presented. A confocal Raman microscope was used to determine the axial confocality in practice. The axial response of a thin planar object was measured for three different objectives, two pinhole sizes and a slit. The results show that, in the case of a confocal configuration, the response calculated with the model provides a good prediction of the axial resolution of the confocal microscope.
Original languageEnglish
Pages (from-to)273-279
Number of pages7
JournalJournal of microscopy
Volume188
Issue number3
DOIs
Publication statusPublished - 1997

Keywords

  • Confocal microscopy
  • Depth resolution

Cite this