Background free CARS imaging by local phase detection

Research output: Chapter in Book/Report/Conference proceedingChapterAcademic

1 Citation (Scopus)


In this article we show that heterodyne CARS, based on a controlled and stable phase-preserving chain, can be used to measure amplitude and phase information of molecular vibration modes. The technique is validated by a comparison of the imaginary part of the heterodyne CARS spectrum to the spontaneous Raman spectrum of polyethylene. The detection of the phase allows for rejection of the non-resonant background from the data. The resulting improvement of the signal to noise ratio is shown by measurements on a sample containing lipid.
Original languageUndefined
Title of host publicationMultiphoton microscopy in the biomedical sciences IX
EditorsAmmasi Periasamy, Peter T.C. So
ISBN (Print)9780819474292
Publication statusPublished - 2009

Publication series

NameProceedings of SPIE
ISSN (Print)1605-7422


  • IR-72659
  • Coherent anti-Stokes Raman scattering
  • Microscopy
  • Nonlinear optics
  • Spectroscopy
  • Phase detection
  • heterodyne

Cite this

Jurna, M., Korterik, J. P., Otto, C., Herek, J. L., & Offerhaus, H. L. (2009). Background free CARS imaging by local phase detection. In A. Periasamy, & P. T. C. So (Eds.), Multiphoton microscopy in the biomedical sciences IX (pp. 718310). (Proceedings of SPIE; Vol. 7183). SPIE.