Background free CARS imaging by local phase detection

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In this article we show that heterodyne CARS, based on a controlled and stable phase-preserving chain, can be used to measure amplitude and phase information of molecular vibration modes. The technique is validated by a comparison of the imaginary part of the heterodyne CARS spectrum to the spontaneous Raman spectrum of polyethylene. The detection of the phase allows for rejection of the non-resonant background from the data. The resulting improvement of the signal to noise ratio is shown by measurements on a sample containing lipid.
Original languageEnglish
Title of host publicationMultiphoton Microscopy in the Biomedical Sciences IX
EditorsAmmasi Periasamy, Peter T.C. So
Place of PublicationSan Jose, CA
ISBN (Print)9780819474292
Publication statusPublished - 2009
EventSPIE International Symposium on Biomedical Optics, BIOS 2009 - San Jose, United States
Duration: 24 Jan 200929 Jan 2009

Publication series

NameProceedings of SPIE
ISSN (Print)1605-7422


ConferenceSPIE International Symposium on Biomedical Optics, BIOS 2009
Country/TerritoryUnited States
CitySan Jose


  • Coherent anti-Stokes Raman scattering
  • Microscopy
  • Nonlinear optics
  • Spectroscopy
  • Phase detection
  • Heterodyne


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