Bacterial Footprints in Elastic Pillared Microstructures

Arturo Susarrey-Arce (Corresponding Author), José Federico Hernández-Sánchez (Corresponding Author), Marco Marcello, Yuri Diaz-Fernandez, Alina Oknianska, Ioritz Sorzabal-Bellido, Roald Tiggelaar, Detlef Lohse, Han Gardeniers, Jacco Snoeijer (Corresponding Author), Alvaro Marin, Rasmita Raval (Corresponding Author)

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Soft substrates decorated with micropillar arrays are known to
be sensitive to deflection due to capillary action. In this work, we demonstrate
that micropillared epoxy surfaces are sensitive to single drops of bacterial
suspensions. The micropillars can show significant deformations upon
evaporation, just as capillary action does in soft substrates. The phenomenon
has been studied with five bacterial strains: S. epidermidis, L. sakei, P.
aeruginosa, E. coli, and B. subtilis. The results reveal that only droplets
containing motile microbes with flagella stimulate micropillar bending, which
leads to significant distortions and pillar aggregations forming dimers, trimers,
and higher order clusters. Such deformation is manifested in characteristic
patterns that are left on the microarrayed surface following evaporation and
can be easily identified even by the naked eye. Our findings could lay the
ground for the design and fabrication of mechanically responsive substrates,
sensitive to specific types of microorganisms.
Original languageEnglish
Pages (from-to)1294-1300
Number of pages7
JournalACS Applied Bio Materials
Volume1
Issue number5
DOIs
Publication statusPublished - 15 Oct 2018

Fingerprint

Capillary Action
Microstructure
Flagella
Substrates
Escherichia coli
Microorganisms
Dimers
Evaporation
Agglomeration
Fabrication

Keywords

  • bacteria
  • bending
  • capillarity
  • elastic micropillars
  • responsive substrates

Cite this

Susarrey-Arce, A., Hernández-Sánchez, J. F., Marcello, M., Diaz-Fernandez, Y., Oknianska, A., Sorzabal-Bellido, I., ... Raval, R. (2018). Bacterial Footprints in Elastic Pillared Microstructures. ACS Applied Bio Materials, 1(5), 1294-1300. https://doi.org/10.1021/acsabm.8b00176
Susarrey-Arce, Arturo ; Hernández-Sánchez, José Federico ; Marcello, Marco ; Diaz-Fernandez, Yuri ; Oknianska, Alina ; Sorzabal-Bellido, Ioritz ; Tiggelaar, Roald ; Lohse, Detlef ; Gardeniers, Han ; Snoeijer, Jacco ; Marin, Alvaro ; Raval, Rasmita. / Bacterial Footprints in Elastic Pillared Microstructures. In: ACS Applied Bio Materials. 2018 ; Vol. 1, No. 5. pp. 1294-1300.
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abstract = "Soft substrates decorated with micropillar arrays are known tobe sensitive to deflection due to capillary action. In this work, we demonstratethat micropillared epoxy surfaces are sensitive to single drops of bacterialsuspensions. The micropillars can show significant deformations uponevaporation, just as capillary action does in soft substrates. The phenomenonhas been studied with five bacterial strains: S. epidermidis, L. sakei, P.aeruginosa, E. coli, and B. subtilis. The results reveal that only dropletscontaining motile microbes with flagella stimulate micropillar bending, whichleads to significant distortions and pillar aggregations forming dimers, trimers,and higher order clusters. Such deformation is manifested in characteristicpatterns that are left on the microarrayed surface following evaporation andcan be easily identified even by the naked eye. Our findings could lay theground for the design and fabrication of mechanically responsive substrates,sensitive to specific types of microorganisms.",
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Susarrey-Arce, A, Hernández-Sánchez, JF, Marcello, M, Diaz-Fernandez, Y, Oknianska, A, Sorzabal-Bellido, I, Tiggelaar, R, Lohse, D, Gardeniers, H, Snoeijer, J, Marin, A & Raval, R 2018, 'Bacterial Footprints in Elastic Pillared Microstructures' ACS Applied Bio Materials, vol. 1, no. 5, pp. 1294-1300. https://doi.org/10.1021/acsabm.8b00176

Bacterial Footprints in Elastic Pillared Microstructures. / Susarrey-Arce, Arturo (Corresponding Author); Hernández-Sánchez, José Federico (Corresponding Author); Marcello, Marco; Diaz-Fernandez, Yuri; Oknianska, Alina; Sorzabal-Bellido, Ioritz; Tiggelaar, Roald; Lohse, Detlef; Gardeniers, Han; Snoeijer, Jacco (Corresponding Author); Marin, Alvaro; Raval, Rasmita (Corresponding Author).

In: ACS Applied Bio Materials, Vol. 1, No. 5, 15.10.2018, p. 1294-1300.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Bacterial Footprints in Elastic Pillared Microstructures

AU - Susarrey-Arce, Arturo

AU - Hernández-Sánchez, José Federico

AU - Marcello, Marco

AU - Diaz-Fernandez, Yuri

AU - Oknianska, Alina

AU - Sorzabal-Bellido, Ioritz

AU - Tiggelaar, Roald

AU - Lohse, Detlef

AU - Gardeniers, Han

AU - Snoeijer, Jacco

AU - Marin, Alvaro

AU - Raval, Rasmita

PY - 2018/10/15

Y1 - 2018/10/15

N2 - Soft substrates decorated with micropillar arrays are known tobe sensitive to deflection due to capillary action. In this work, we demonstratethat micropillared epoxy surfaces are sensitive to single drops of bacterialsuspensions. The micropillars can show significant deformations uponevaporation, just as capillary action does in soft substrates. The phenomenonhas been studied with five bacterial strains: S. epidermidis, L. sakei, P.aeruginosa, E. coli, and B. subtilis. The results reveal that only dropletscontaining motile microbes with flagella stimulate micropillar bending, whichleads to significant distortions and pillar aggregations forming dimers, trimers,and higher order clusters. Such deformation is manifested in characteristicpatterns that are left on the microarrayed surface following evaporation andcan be easily identified even by the naked eye. Our findings could lay theground for the design and fabrication of mechanically responsive substrates,sensitive to specific types of microorganisms.

AB - Soft substrates decorated with micropillar arrays are known tobe sensitive to deflection due to capillary action. In this work, we demonstratethat micropillared epoxy surfaces are sensitive to single drops of bacterialsuspensions. The micropillars can show significant deformations uponevaporation, just as capillary action does in soft substrates. The phenomenonhas been studied with five bacterial strains: S. epidermidis, L. sakei, P.aeruginosa, E. coli, and B. subtilis. The results reveal that only dropletscontaining motile microbes with flagella stimulate micropillar bending, whichleads to significant distortions and pillar aggregations forming dimers, trimers,and higher order clusters. Such deformation is manifested in characteristicpatterns that are left on the microarrayed surface following evaporation andcan be easily identified even by the naked eye. Our findings could lay theground for the design and fabrication of mechanically responsive substrates,sensitive to specific types of microorganisms.

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JF - ACS Applied Bio Materials

SN - 2576-6422

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ER -

Susarrey-Arce A, Hernández-Sánchez JF, Marcello M, Diaz-Fernandez Y, Oknianska A, Sorzabal-Bellido I et al. Bacterial Footprints in Elastic Pillared Microstructures. ACS Applied Bio Materials. 2018 Oct 15;1(5):1294-1300. https://doi.org/10.1021/acsabm.8b00176