Bacterial Footprints in Elastic Pillared Microstructures

Arturo Susarrey-Arce (Corresponding Author), José Federico Hernández-Sánchez (Corresponding Author), Marco Marcello, Yuri Diaz-Fernandez, Alina Oknianska, Ioritz Sorzabal-Bellido, Roald Tiggelaar, Detlef Lohse, Han Gardeniers, Jacco Snoeijer (Corresponding Author), Alvaro Marin, Rasmita Raval (Corresponding Author)

Research output: Contribution to journalArticleAcademicpeer-review

3 Citations (Scopus)

Abstract

Soft substrates decorated with micropillar arrays are known to
be sensitive to deflection due to capillary action. In this work, we demonstrate
that micropillared epoxy surfaces are sensitive to single drops of bacterial
suspensions. The micropillars can show significant deformations upon
evaporation, just as capillary action does in soft substrates. The phenomenon
has been studied with five bacterial strains: S. epidermidis, L. sakei, P.
aeruginosa, E. coli, and B. subtilis. The results reveal that only droplets
containing motile microbes with flagella stimulate micropillar bending, which
leads to significant distortions and pillar aggregations forming dimers, trimers,
and higher order clusters. Such deformation is manifested in characteristic
patterns that are left on the microarrayed surface following evaporation and
can be easily identified even by the naked eye. Our findings could lay the
ground for the design and fabrication of mechanically responsive substrates,
sensitive to specific types of microorganisms.
Original languageEnglish
Pages (from-to)1294-1300
Number of pages7
JournalACS Applied Bio Materials
Volume1
Issue number5
DOIs
Publication statusPublished - 15 Oct 2018

Keywords

  • bacteria
  • bending
  • capillarity
  • elastic micropillars
  • responsive substrates

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  • Cite this

    Susarrey-Arce, A., Hernández-Sánchez, J. F., Marcello, M., Diaz-Fernandez, Y., Oknianska, A., Sorzabal-Bellido, I., ... Raval, R. (2018). Bacterial Footprints in Elastic Pillared Microstructures. ACS Applied Bio Materials, 1(5), 1294-1300. https://doi.org/10.1021/acsabm.8b00176