Bacterial Footprints in Elastic Pillared Microstructures

Arturo Susarrey-Arce; José Federico Hernández-Sánchez; Marco Marcello; Yuri Diaz-Fernandez; Alina Oknianska; Ioritz Sorzabal-Bellido; Roald Tiggelaar; Detlef Lohse; Han Gardeniers; Jacco Snoeijer; Alvaro Marin; Rasmita Raval

doi:10.1021/acsabm.8b00176

Bacterial Footprints in Elastic Pillared Microstructures

Arturo Susarrey-Arce (Corresponding Author), José Federico Hernández-Sánchez (Corresponding Author), Marco Marcello, Yuri Diaz-Fernandez, Alina Oknianska, Ioritz Sorzabal-Bellido, Roald Tiggelaar, Detlef Lohse, Han Gardeniers, Jacco Snoeijer (Corresponding Author), Alvaro Marin, Rasmita Raval (Corresponding Author)

Research output: Contribution to journal › Article › Academic › peer-review

9 Citations (Scopus)

69 Downloads (Pure)

Abstract

Soft substrates decorated with micropillar arrays are known to
be sensitive to deflection due to capillary action. In this work, we demonstrate
that micropillared epoxy surfaces are sensitive to single drops of bacterial
suspensions. The micropillars can show significant deformations upon
evaporation, just as capillary action does in soft substrates. The phenomenon
has been studied with five bacterial strains: S. epidermidis, L. sakei, P.
aeruginosa, E. coli, and B. subtilis. The results reveal that only droplets
containing motile microbes with flagella stimulate micropillar bending, which
leads to significant distortions and pillar aggregations forming dimers, trimers,
and higher order clusters. Such deformation is manifested in characteristic
patterns that are left on the microarrayed surface following evaporation and
can be easily identified even by the naked eye. Our findings could lay the
ground for the design and fabrication of mechanically responsive substrates,
sensitive to specific types of microorganisms.

Original language	English
Pages (from-to)	1294-1300
Number of pages	7
Journal	ACS Applied Bio Materials
Volume	1
Issue number	5
DOIs	https://doi.org/10.1021/acsabm.8b00176
Publication status	Published - 15 Oct 2018

Keywords

bacteria
bending
capillarity
elastic micropillars
responsive substrates
22/4 OA procedure

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10.1021/acsabm.8b00176

ArticleFinal published version, 8.06 MBLicence: Taverne

9 Citations
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Bacterial Footprints in Elastic Pillared Microstructures
Susarrey-Arce, A., Hernandez-Sanchez, J. F., Marcello, M., Diaz-Fernandez, Y., Oknianska, A., Sorzabal-Bellido, I., Tiggelaar, R., Lohse, D., Gardeniers, H., Snoeijer, J., Marin, A. & Raval, R., 14 Sept 2019, ArXiv.org.
Research output: Working paper › Preprint › Academic

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Cite this

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title = "Bacterial Footprints in Elastic Pillared Microstructures",

abstract = "Soft substrates decorated with micropillar arrays are known tobe sensitive to deflection due to capillary action. In this work, we demonstratethat micropillared epoxy surfaces are sensitive to single drops of bacterialsuspensions. The micropillars can show significant deformations uponevaporation, just as capillary action does in soft substrates. The phenomenonhas been studied with five bacterial strains: S. epidermidis, L. sakei, P.aeruginosa, E. coli, and B. subtilis. The results reveal that only dropletscontaining motile microbes with flagella stimulate micropillar bending, whichleads to significant distortions and pillar aggregations forming dimers, trimers,and higher order clusters. Such deformation is manifested in characteristicpatterns that are left on the microarrayed surface following evaporation andcan be easily identified even by the naked eye. Our findings could lay theground for the design and fabrication of mechanically responsive substrates,sensitive to specific types of microorganisms.",

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doi = "10.1021/acsabm.8b00176",

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AU - Susarrey-Arce, Arturo

AU - Hernández-Sánchez, José Federico

AU - Marcello, Marco

AU - Diaz-Fernandez, Yuri

AU - Oknianska, Alina

AU - Sorzabal-Bellido, Ioritz

AU - Tiggelaar, Roald

AU - Lohse, Detlef

AU - Gardeniers, Han

AU - Snoeijer, Jacco

AU - Marin, Alvaro

AU - Raval, Rasmita

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N2 - Soft substrates decorated with micropillar arrays are known tobe sensitive to deflection due to capillary action. In this work, we demonstratethat micropillared epoxy surfaces are sensitive to single drops of bacterialsuspensions. The micropillars can show significant deformations uponevaporation, just as capillary action does in soft substrates. The phenomenonhas been studied with five bacterial strains: S. epidermidis, L. sakei, P.aeruginosa, E. coli, and B. subtilis. The results reveal that only dropletscontaining motile microbes with flagella stimulate micropillar bending, whichleads to significant distortions and pillar aggregations forming dimers, trimers,and higher order clusters. Such deformation is manifested in characteristicpatterns that are left on the microarrayed surface following evaporation andcan be easily identified even by the naked eye. Our findings could lay theground for the design and fabrication of mechanically responsive substrates,sensitive to specific types of microorganisms.

AB - Soft substrates decorated with micropillar arrays are known tobe sensitive to deflection due to capillary action. In this work, we demonstratethat micropillared epoxy surfaces are sensitive to single drops of bacterialsuspensions. The micropillars can show significant deformations uponevaporation, just as capillary action does in soft substrates. The phenomenonhas been studied with five bacterial strains: S. epidermidis, L. sakei, P.aeruginosa, E. coli, and B. subtilis. The results reveal that only dropletscontaining motile microbes with flagella stimulate micropillar bending, whichleads to significant distortions and pillar aggregations forming dimers, trimers,and higher order clusters. Such deformation is manifested in characteristicpatterns that are left on the microarrayed surface following evaporation andcan be easily identified even by the naked eye. Our findings could lay theground for the design and fabrication of mechanically responsive substrates,sensitive to specific types of microorganisms.

KW - bacteria

KW - bending

KW - capillarity

KW - elastic micropillars

KW - responsive substrates

KW - 22/4 OA procedure

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JO - ACS Applied Bio Materials

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ER -

Bacterial Footprints in Elastic Pillared Microstructures

Abstract

Keywords

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Bacterial Footprints in Elastic Pillared Microstructures

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