TY - JOUR
T1 - Bactericidal Characteristics of Bioinspired Nontoxic and Chemically Stable Disordered Silicon Nanopyramids
AU - Saini, Sudhir K.
AU - Halder, Moumita
AU - Singh, Yashveer
AU - Nair, Rajesh V.
N1 - Funding Information:
The financial support from INSA (SP/YSP/135/2016/1064) and CSIR (no. 03(1352)/16/EMR-II) to R.V.N., and SERB, New Delhi, (EMR/2017/000045) to Y.S. is gratefully acknowledged.
Funding Information:
The authors acknowledge IIT Ropar for giving access to SEM and AFM in the central research facilities of the institute. S.K.S. thanks MeitY, Govt of India, for the Visvesvaraya Ph.D. fellowship and M.H. thanks IIT Ropar for the institute fellowship. We also thank Dr. Kamal Malhotra for helping with the protocols used in the antibacterial studies.
Publisher Copyright:
Copyright © 2020 American Chemical Society.
PY - 2020/5/11
Y1 - 2020/5/11
N2 - Controlling bacterial growth using artificial nanostructures inspired from natural species is of immense importance in biomedical applications. In the present work, a low cost, fast processing, and scalable anisotropic wet etching technique is developed to fabricate the densely packed disordered silicon nanopyramids (SiNPs) with nanosized sharp tips. The bactericidal characteristics of SiNPs are assessed against strains implicated in nosocomial and biomaterial-related infections. Compared to the bare silicon with no antibacterial activities, SiNPs of 1.85 ± 0.28 μm height show 55 and 75% inhibition of Escherichia coli (Gram-negative) and Bacillus subtilis (Gram-positive) bacteria, whereas the silicon nanowires (SiNWs) fabricated using a metal-assisted chemical etching method show 50 and 58% inhibition of E. coli and B. subtilis. The mechanistic studies using a scanning electron microscope and live/dead bacterial cell assay reveal cell rupture and predominance of dead cells on contact with SiNPs and SiNWs, which confirms their bactericidal effects. Chemical stability and cell viability studies demonstrate the biocompatible nature of SiNP and SiNW surfaces. Owing to their capability to kill both Gram-negative and positive bacteria and minimal toxicity to murine fibroblast cells, SiNPs can be used as an antibacterial coating on medical devices to prevent nosocomial and biomaterial-related infections.
AB - Controlling bacterial growth using artificial nanostructures inspired from natural species is of immense importance in biomedical applications. In the present work, a low cost, fast processing, and scalable anisotropic wet etching technique is developed to fabricate the densely packed disordered silicon nanopyramids (SiNPs) with nanosized sharp tips. The bactericidal characteristics of SiNPs are assessed against strains implicated in nosocomial and biomaterial-related infections. Compared to the bare silicon with no antibacterial activities, SiNPs of 1.85 ± 0.28 μm height show 55 and 75% inhibition of Escherichia coli (Gram-negative) and Bacillus subtilis (Gram-positive) bacteria, whereas the silicon nanowires (SiNWs) fabricated using a metal-assisted chemical etching method show 50 and 58% inhibition of E. coli and B. subtilis. The mechanistic studies using a scanning electron microscope and live/dead bacterial cell assay reveal cell rupture and predominance of dead cells on contact with SiNPs and SiNWs, which confirms their bactericidal effects. Chemical stability and cell viability studies demonstrate the biocompatible nature of SiNP and SiNW surfaces. Owing to their capability to kill both Gram-negative and positive bacteria and minimal toxicity to murine fibroblast cells, SiNPs can be used as an antibacterial coating on medical devices to prevent nosocomial and biomaterial-related infections.
KW - antibacterial surface
KW - bioinspired nanostructures
KW - biomaterial-related infections
KW - silicon nanopyramids
KW - silicon nanowires
UR - http://www.scopus.com/inward/record.url?scp=85088919176&partnerID=8YFLogxK
U2 - 10.1021/acsbiomaterials.9b01963
DO - 10.1021/acsbiomaterials.9b01963
M3 - Article
C2 - 33463264
AN - SCOPUS:85088919176
SN - 2373-9878
VL - 6
SP - 2778
EP - 2786
JO - ACS Biomaterials Science and Engineering
JF - ACS Biomaterials Science and Engineering
IS - 5
ER -