Barbed channels enhance unidirectional connectivity between neuronal networks cultured on multi electrode arrays.

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Abstract

Cultured neurons on multi electrode arrays (MEAs) have been widely used to study various as-pects of neuronal (network) functioning. A possible drawback of this approach is the lack of struc-ture in these networks. At the single cell level, several solutions have been proposed to enable di-rected connectivity, and promising results were obtained. At the level of connected sub-populations, a few attempts have been made with promising results. First assessment of the de-signs’ functionality, however, suggested room for further improvement. We designed a two chamber MEA aiming to create a unidirectional connection between the net-works in both chambers (‘emitting’ and ‘receiving’). To achieve this unidirectionality, all intercon-necting channels contained barbs that hindered axon growth in the opposite direction (from receiv-ing to emitting chamber). Visual inspection showed that axons predominantly grew through the channels in the promoted direction. This observation was confirmed by spontaneous activity re-cordings. Cross-correlation between the signals from two electrodes inside the channels suggested signal propagation at ≈2 m/s from emitting to receiving chamber. Cross-correlation between the fir-ing patterns in both chambers indicated that most correlated activity was initiated in the emitting chamber, which was also reflected by a significantly lower fraction of partial bursts (e. a one-chamber-only burst) in the emitting chamber. Finally, electrical stimulation in the emitting chamber induced a fast response in that chamber, and a slower response in the receiving chamber. Stimula-tion in the receiving chamber evoked a fast response in that chamber, but no response in the emit-ting chamber. These results confirm the predominantly unidirectional nature of the connecting channels from emitting to receiving chamber.
Original languageUndefined
Pages (from-to)00412-00412
Number of pages10
JournalFrontiers in neuroscience
Volume9
Issue number412
DOIs
Publication statusPublished - 3 Nov 2015

Keywords

  • METIS-315074
  • Cortical neurons
  • Multi electrode array
  • IR-98611
  • EWI-26529
  • stimulus response
  • Electrophysiology
  • Electrical stimulation
  • BSS-Neurotechnology and cellular engineering
  • spontaneous activity

Cite this

@article{37b29e86281042b4afee16a59f7918d1,
title = "Barbed channels enhance unidirectional connectivity between neuronal networks cultured on multi electrode arrays.",
abstract = "Cultured neurons on multi electrode arrays (MEAs) have been widely used to study various as-pects of neuronal (network) functioning. A possible drawback of this approach is the lack of struc-ture in these networks. At the single cell level, several solutions have been proposed to enable di-rected connectivity, and promising results were obtained. At the level of connected sub-populations, a few attempts have been made with promising results. First assessment of the de-signs’ functionality, however, suggested room for further improvement. We designed a two chamber MEA aiming to create a unidirectional connection between the net-works in both chambers (‘emitting’ and ‘receiving’). To achieve this unidirectionality, all intercon-necting channels contained barbs that hindered axon growth in the opposite direction (from receiv-ing to emitting chamber). Visual inspection showed that axons predominantly grew through the channels in the promoted direction. This observation was confirmed by spontaneous activity re-cordings. Cross-correlation between the signals from two electrodes inside the channels suggested signal propagation at ≈2 m/s from emitting to receiving chamber. Cross-correlation between the fir-ing patterns in both chambers indicated that most correlated activity was initiated in the emitting chamber, which was also reflected by a significantly lower fraction of partial bursts (e. a one-chamber-only burst) in the emitting chamber. Finally, electrical stimulation in the emitting chamber induced a fast response in that chamber, and a slower response in the receiving chamber. Stimula-tion in the receiving chamber evoked a fast response in that chamber, but no response in the emit-ting chamber. These results confirm the predominantly unidirectional nature of the connecting channels from emitting to receiving chamber.",
keywords = "METIS-315074, Cortical neurons, Multi electrode array, IR-98611, EWI-26529, stimulus response, Electrophysiology, Electrical stimulation, BSS-Neurotechnology and cellular engineering, spontaneous activity",
author = "{le Feber}, Jakob and W. Postma and {de Weerd}, {Eddy L} and Weusthof, {Marcel H.H.} and Wim Rutten",
note = "Open Access",
year = "2015",
month = "11",
day = "3",
doi = "10.3389/fnins.2015.00412",
language = "Undefined",
volume = "9",
pages = "00412--00412",
journal = "Frontiers in neuroscience",
issn = "1662-4548",
publisher = "Frontiers Media S.A.",
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}

Barbed channels enhance unidirectional connectivity between neuronal networks cultured on multi electrode arrays. / le Feber, Jakob; Postma, W.; de Weerd, Eddy L; Weusthof, Marcel H.H.; Rutten, Wim.

In: Frontiers in neuroscience, Vol. 9, No. 412, 03.11.2015, p. 00412-00412.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Barbed channels enhance unidirectional connectivity between neuronal networks cultured on multi electrode arrays.

AU - le Feber, Jakob

AU - Postma, W.

AU - de Weerd, Eddy L

AU - Weusthof, Marcel H.H.

AU - Rutten, Wim

N1 - Open Access

PY - 2015/11/3

Y1 - 2015/11/3

N2 - Cultured neurons on multi electrode arrays (MEAs) have been widely used to study various as-pects of neuronal (network) functioning. A possible drawback of this approach is the lack of struc-ture in these networks. At the single cell level, several solutions have been proposed to enable di-rected connectivity, and promising results were obtained. At the level of connected sub-populations, a few attempts have been made with promising results. First assessment of the de-signs’ functionality, however, suggested room for further improvement. We designed a two chamber MEA aiming to create a unidirectional connection between the net-works in both chambers (‘emitting’ and ‘receiving’). To achieve this unidirectionality, all intercon-necting channels contained barbs that hindered axon growth in the opposite direction (from receiv-ing to emitting chamber). Visual inspection showed that axons predominantly grew through the channels in the promoted direction. This observation was confirmed by spontaneous activity re-cordings. Cross-correlation between the signals from two electrodes inside the channels suggested signal propagation at ≈2 m/s from emitting to receiving chamber. Cross-correlation between the fir-ing patterns in both chambers indicated that most correlated activity was initiated in the emitting chamber, which was also reflected by a significantly lower fraction of partial bursts (e. a one-chamber-only burst) in the emitting chamber. Finally, electrical stimulation in the emitting chamber induced a fast response in that chamber, and a slower response in the receiving chamber. Stimula-tion in the receiving chamber evoked a fast response in that chamber, but no response in the emit-ting chamber. These results confirm the predominantly unidirectional nature of the connecting channels from emitting to receiving chamber.

AB - Cultured neurons on multi electrode arrays (MEAs) have been widely used to study various as-pects of neuronal (network) functioning. A possible drawback of this approach is the lack of struc-ture in these networks. At the single cell level, several solutions have been proposed to enable di-rected connectivity, and promising results were obtained. At the level of connected sub-populations, a few attempts have been made with promising results. First assessment of the de-signs’ functionality, however, suggested room for further improvement. We designed a two chamber MEA aiming to create a unidirectional connection between the net-works in both chambers (‘emitting’ and ‘receiving’). To achieve this unidirectionality, all intercon-necting channels contained barbs that hindered axon growth in the opposite direction (from receiv-ing to emitting chamber). Visual inspection showed that axons predominantly grew through the channels in the promoted direction. This observation was confirmed by spontaneous activity re-cordings. Cross-correlation between the signals from two electrodes inside the channels suggested signal propagation at ≈2 m/s from emitting to receiving chamber. Cross-correlation between the fir-ing patterns in both chambers indicated that most correlated activity was initiated in the emitting chamber, which was also reflected by a significantly lower fraction of partial bursts (e. a one-chamber-only burst) in the emitting chamber. Finally, electrical stimulation in the emitting chamber induced a fast response in that chamber, and a slower response in the receiving chamber. Stimula-tion in the receiving chamber evoked a fast response in that chamber, but no response in the emit-ting chamber. These results confirm the predominantly unidirectional nature of the connecting channels from emitting to receiving chamber.

KW - METIS-315074

KW - Cortical neurons

KW - Multi electrode array

KW - IR-98611

KW - EWI-26529

KW - stimulus response

KW - Electrophysiology

KW - Electrical stimulation

KW - BSS-Neurotechnology and cellular engineering

KW - spontaneous activity

U2 - 10.3389/fnins.2015.00412

DO - 10.3389/fnins.2015.00412

M3 - Article

VL - 9

SP - 412

EP - 412

JO - Frontiers in neuroscience

JF - Frontiers in neuroscience

SN - 1662-4548

IS - 412

ER -