Barbed channels enhance unidirectional connectivity between neuronal networks cultured on multi electrode arrays

Joost le Feber, Wybren Postma, Eddy de Weerd, Marcel Weusthof, Wim L.C. Rutten

Research output: Contribution to journalArticleAcademicpeer-review

33 Citations (Scopus)
112 Downloads (Pure)

Abstract

Cultured neurons on multi electrode arrays (MEAs) have been widely used to study various as-pects of neuronal (network) functioning. A possible drawback of this approach is the lack of struc-ture in these networks. At the single cell level, several solutions have been proposed to enable di-rected connectivity, and promising results were obtained. At the level of connected sub-populations, a few attempts have been made with promising results. First assessment of the de-signs’ functionality, however, suggested room for further improvement. We designed a two chamber MEA aiming to create a unidirectional connection between the net-works in both chambers (‘emitting’ and ‘receiving’). To achieve this unidirectionality, all intercon-necting channels contained barbs that hindered axon growth in the opposite direction (from receiv-ing to emitting chamber). Visual inspection showed that axons predominantly grew through the channels in the promoted direction. This observation was confirmed by spontaneous activity re-cordings. Cross-correlation between the signals from two electrodes inside the channels suggested signal propagation at ≈2 m/s from emitting to receiving chamber. Cross-correlation between the fir-ing patterns in both chambers indicated that most correlated activity was initiated in the emitting chamber, which was also reflected by a significantly lower fraction of partial bursts (e. a one-chamber-only burst) in the emitting chamber. Finally, electrical stimulation in the emitting chamber induced a fast response in that chamber, and a slower response in the receiving chamber. Stimula-tion in the receiving chamber evoked a fast response in that chamber, but no response in the emit-ting chamber. These results confirm the predominantly unidirectional nature of the connecting channels from emitting to receiving chamber.
Original languageEnglish
Article number412
Number of pages10
JournalFrontiers in Neuroscience
Volume9
DOIs
Publication statusPublished - 3 Nov 2015

Keywords

  • Cortical neurons
  • Multi electrode array
  • stimulus response
  • Electrophysiology
  • Electrical stimulation
  • BSS-Neurotechnology and cellular engineering
  • spontaneous activity

Fingerprint

Dive into the research topics of 'Barbed channels enhance unidirectional connectivity between neuronal networks cultured on multi electrode arrays'. Together they form a unique fingerprint.

Cite this