Bessel-beam Grueneisen relaxation photoacoustic microscopy with extended depth of field

Junhui Shi, Lidai Wang, Cedric Noordam, Lihong V. Wang

Research output: Contribution to journalArticleAcademicpeer-review

33 Citations (Scopus)


The short focal depth of a Gaussian beam limits the volumetric imaging speed of optical resolution photoacoustic microscopy (OR-PAM). A Bessel beam, which is diffraction free, provides a long focal depth, but its side lobes deteriorate image quality when the Bessel beam is directly employed to excite photoacoustic (PA) signals in OR-PAM. We present a nonlinear approach based on the Grueneisen relaxation effect to suppress the side-lobe artifacts in PA imaging. This method extends the focal depth of OR-PAM and speeds up volumetric imaging. We experimentally demonstrated a 1-mm focal depth with a 7-μm lateral resolution and volumetrically imaged a carbon fiber and red blood cell samples.
Original languageEnglish
Article number116002
Pages (from-to)116002-
JournalJournal of biomedical optics
Issue number11
Publication statusPublished - 2015


  • IR-98593
  • METIS-313089


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