Bessel-beam Grueneisen relaxation photoacoustic microscopy with extended depth of field

Junhui Shi, Lidai Wang, Cedric Noordam, Lihong V. Wang

Research output: Contribution to journalArticleAcademicpeer-review

13 Citations (Scopus)

Abstract

The short focal depth of a Gaussian beam limits the volumetric imaging speed of optical resolution photoacoustic microscopy (OR-PAM). A Bessel beam, which is diffraction free, provides a long focal depth, but its side lobes deteriorate image quality when the Bessel beam is directly employed to excite photoacoustic (PA) signals in OR-PAM. We present a nonlinear approach based on the Grueneisen relaxation effect to suppress the side-lobe artifacts in PA imaging. This method extends the focal depth of OR-PAM and speeds up volumetric imaging. We experimentally demonstrated a 1-mm focal depth with a 7-μm lateral resolution and volumetrically imaged a carbon fiber and red blood cell samples.
Original languageEnglish
Article number116002
Pages (from-to)116002-
JournalJournal of biomedical optics
Volume20
Issue number11
DOIs
Publication statusPublished - 2015

Fingerprint

Photoacoustic microscopy
photoacoustic microscopy
Photoacoustic effect
Imaging techniques
lobes
Gaussian beams
Image quality
Carbon fibers
erythrocytes
Blood
carbon fibers
Diffraction
Cells
artifacts
diffraction

Keywords

  • IR-98593
  • METIS-313089

Cite this

Shi, Junhui ; Wang, Lidai ; Noordam, Cedric ; Wang, Lihong V. / Bessel-beam Grueneisen relaxation photoacoustic microscopy with extended depth of field. In: Journal of biomedical optics. 2015 ; Vol. 20, No. 11. pp. 116002-.
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Bessel-beam Grueneisen relaxation photoacoustic microscopy with extended depth of field. / Shi, Junhui; Wang, Lidai; Noordam, Cedric; Wang, Lihong V.

In: Journal of biomedical optics, Vol. 20, No. 11, 116002, 2015, p. 116002-.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - Bessel-beam Grueneisen relaxation photoacoustic microscopy with extended depth of field

AU - Shi, Junhui

AU - Wang, Lidai

AU - Noordam, Cedric

AU - Wang, Lihong V.

PY - 2015

Y1 - 2015

N2 - The short focal depth of a Gaussian beam limits the volumetric imaging speed of optical resolution photoacoustic microscopy (OR-PAM). A Bessel beam, which is diffraction free, provides a long focal depth, but its side lobes deteriorate image quality when the Bessel beam is directly employed to excite photoacoustic (PA) signals in OR-PAM. We present a nonlinear approach based on the Grueneisen relaxation effect to suppress the side-lobe artifacts in PA imaging. This method extends the focal depth of OR-PAM and speeds up volumetric imaging. We experimentally demonstrated a 1-mm focal depth with a 7-μm lateral resolution and volumetrically imaged a carbon fiber and red blood cell samples.

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KW - IR-98593

KW - METIS-313089

U2 - 10.1117/1.JBO.20.11.116002

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