Bioanalysis and pharmacokinetics of the p38 MAPkinase inhibitor SB202190 in rats

Jai Prakash*, Vinay Saluja, Jan Visser, Frits Moolenaar, Dirk K.F. Meijer, Klaas Poelstra, Robbert J. Kok

*Corresponding author for this work

    Research output: Contribution to journalArticleAcademicpeer-review

    6 Citations (Scopus)

    Abstract

    We have developed a sensitive and reproducible high performance liquid chromatography (HPLC)-UV method for the quantification of the p38 MAPkinase inhibitor SB202190 in serum, kidney homogenates and urine samples. Liquid-liquid extraction of SB202190 from the samples was performed using diethylether after adding a derivative of SB202190 as internal standard (I.S.). Chromatography was carried out using a C8 reversed-phase column with an isocratic mobile phase consisting of acetonitrile-water-trifluoroacetic acid (30:70:0.1, v/v/v; pH 2.0). Both drug and I.S. were measured at 350 nm and eluted at 5.0 and 10.6 min, respectively. Peak-height ratios of the drug and the I.S. were used for the quantification of SB202190 from the different matrixes. The limit of quantitation of SB202190 in serum, kidney and urine were 0.25 μg/ml, 1 μg/g and 1 μg/ml, respectively. The average recoveries were 74, 75 and 92% in serum, kidney and urine, respectively. The intra- and inter-day precision (% CV) and accuracy (% bias) were below 15% for all concentrations. The method was successfully applied for a pharmacokinetic study of SB202190 in rats.

    Original languageEnglish
    Pages (from-to)220-225
    Number of pages6
    JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
    Volume826
    Issue number1-2
    DOIs
    Publication statusPublished - 5 Nov 2005

    Keywords

    • Drug analysis
    • High performance liquid chromatography
    • Liquid-liquid extraction
    • Pharmacokinetic studies
    • Renal fibrosis

    Fingerprint Dive into the research topics of 'Bioanalysis and pharmacokinetics of the p38 MAPkinase inhibitor SB202190 in rats'. Together they form a unique fingerprint.

    Cite this