Biofilm development in time on a silicone voice prosthesis - a case study

T.R. Neu; C.E. de Boer; G.J. Verkerke; H.K. Schutte; G. Rakhorst; H.C. van der Mei; H.J. Busscher

doi:10.3109/08910609409141571

Biofilm development in time on a silicone voice prosthesis - a case study

T.R. Neu^*, C.E. de Boer, G.J. Verkerke, H.K. Schutte, G. Rakhorst, H.C. van der Mei, H.J. Busscher

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

Voice prostheses from silicone elastomers become rapidly colonised by a mixed biofilm of bacteria and yeasts. In this study, microorganisms were isolated from biofilms on explanted prostheses after having been in place for various time intervals ranging from 1 to 67 d. The isolates were examined for their identity, adhesion to hexadecane and electro-phoretic mobility. Bacteria from early (shorter than 8 d) and late (longer than 8 d) explants could not be classified according to their taxonomy, hydrophobicity or electrophoretic mobility. However, the yeasts clearly revealed a dominance of only hydrophilic Candida albicans isolates from early explants and only hydrophobic C. tropicalis isolates from late explants. These findings may be of significance for the development of strategies to control mixed biofilms on biomaterials.

Original language	English
Pages (from-to)	27-33
Number of pages	7
Journal	Microbial Ecology in Health and Disease
Volume	7
Issue number	1
DOIs	https://doi.org/10.3109/08910609409141571
Publication status	Published - 1 Jan 1994
Externally published	Yes

Keywords

Adhesion
Bacteria
Biofilms
Biomaterials
Candida
Colonisation
Silicone elastomers
Time study
Voice prostheses
Yeasts

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Neu1993biofilmFinal published version, 1.73 MBLicence: CC BY

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title = "Biofilm development in time on a silicone voice prosthesis - a case study",

abstract = "Voice prostheses from silicone elastomers become rapidly colonised by a mixed biofilm of bacteria and yeasts. In this study, microorganisms were isolated from biofilms on explanted prostheses after having been in place for various time intervals ranging from 1 to 67 d. The isolates were examined for their identity, adhesion to hexadecane and electro-phoretic mobility. Bacteria from early (shorter than 8 d) and late (longer than 8 d) explants could not be classified according to their taxonomy, hydrophobicity or electrophoretic mobility. However, the yeasts clearly revealed a dominance of only hydrophilic Candida albicans isolates from early explants and only hydrophobic C. tropicalis isolates from late explants. These findings may be of significance for the development of strategies to control mixed biofilms on biomaterials.",

keywords = "Adhesion, Bacteria, Biofilms, Biomaterials, Candida, Colonisation, Silicone elastomers, Time study, Voice prostheses, Yeasts",

author = "T.R. Neu and {de Boer}, C.E. and G.J. Verkerke and H.K. Schutte and G. Rakhorst and {van der Mei}, H.C. and H.J. Busscher",

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AU - Neu, T.R.

AU - de Boer, C.E.

AU - Verkerke, G.J.

AU - Schutte, H.K.

AU - Rakhorst, G.

AU - van der Mei, H.C.

AU - Busscher, H.J.

PY - 1994/1/1

Y1 - 1994/1/1

N2 - Voice prostheses from silicone elastomers become rapidly colonised by a mixed biofilm of bacteria and yeasts. In this study, microorganisms were isolated from biofilms on explanted prostheses after having been in place for various time intervals ranging from 1 to 67 d. The isolates were examined for their identity, adhesion to hexadecane and electro-phoretic mobility. Bacteria from early (shorter than 8 d) and late (longer than 8 d) explants could not be classified according to their taxonomy, hydrophobicity or electrophoretic mobility. However, the yeasts clearly revealed a dominance of only hydrophilic Candida albicans isolates from early explants and only hydrophobic C. tropicalis isolates from late explants. These findings may be of significance for the development of strategies to control mixed biofilms on biomaterials.

AB - Voice prostheses from silicone elastomers become rapidly colonised by a mixed biofilm of bacteria and yeasts. In this study, microorganisms were isolated from biofilms on explanted prostheses after having been in place for various time intervals ranging from 1 to 67 d. The isolates were examined for their identity, adhesion to hexadecane and electro-phoretic mobility. Bacteria from early (shorter than 8 d) and late (longer than 8 d) explants could not be classified according to their taxonomy, hydrophobicity or electrophoretic mobility. However, the yeasts clearly revealed a dominance of only hydrophilic Candida albicans isolates from early explants and only hydrophobic C. tropicalis isolates from late explants. These findings may be of significance for the development of strategies to control mixed biofilms on biomaterials.

KW - Adhesion

KW - Bacteria

KW - Biofilms

KW - Biomaterials

KW - Candida

KW - Colonisation

KW - Silicone elastomers

KW - Time study

KW - Voice prostheses

KW - Yeasts

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JO - Microbial Ecology in Health and Disease

JF - Microbial Ecology in Health and Disease

IS - 1

ER -

Biofilm development in time on a silicone voice prosthesis - a case study

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Keywords

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