Biological characterisation of vascular grafts cultured in a bioreactor

P. Engbers-Buijtenhuijs, L. Buttafoco, Andreas A. Poot, Pieter J. Dijkstra, Rob A.I. de Vos, Lotus M.Th. Sterk, Rob H. Geelkerken, I. Vermes, Jan Feijen

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Abstract

In this study, the development is described of a tissue-engineered construct mimicking the structure of a natural blood vessel. Smooth muscle cells (SMC) were cultured under pulsatile flow conditions in porous tubular scaffolds composed of crosslinked type I insoluble collagen and insoluble elastin. Under these dynamic culture conditions, average wall shear rate, systolic and diastolic pressures and pressure wave-forms comparable to conditions in the human carotid artery were obtained. Culturing of SMC in tubular scaffolds under dynamic conditions resulted in enhanced tissue formation compared to static conditions. Higher SMC numbers, a more homogeneous distribution of SMC throughout the scaffolds and higher collagen mRNA expression levels were found when cells were cultured under dynamic compared to static conditions. mRNA expression levels of markers of proliferation and apoptosis showed that the higher cell numbers in the scaffolds cultured under dynamic conditions can be explained by increased cell proliferation but not by decreased apoptosis. Glucose consumption and lactate formation by the cells showed that cell metabolism was more aerobic under dynamic compared to static conditions. Lining of the dynamically cultured constructs with a luminal monolayer of endothelial cells might result in vessels suitable for in vivo applications.
Original languageUndefined
Pages (from-to)2390-2397
JournalBiomaterials
Volume27
Issue number11
DOIs
Publication statusPublished - 2006

Keywords

  • Vascular tissue engineering
  • Elastin
  • Smooth muscle cells
  • Collagen
  • IR-67198
  • Cell proliferation
  • Apoptosis
  • METIS-229842

Cite this

Engbers-Buijtenhuijs, P. ; Buttafoco, L. ; Poot, Andreas A. ; Dijkstra, Pieter J. ; de Vos, Rob A.I. ; Sterk, Lotus M.Th. ; Geelkerken, Rob H. ; Vermes, I. ; Feijen, Jan. / Biological characterisation of vascular grafts cultured in a bioreactor. In: Biomaterials. 2006 ; Vol. 27, No. 11. pp. 2390-2397.
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title = "Biological characterisation of vascular grafts cultured in a bioreactor",
abstract = "In this study, the development is described of a tissue-engineered construct mimicking the structure of a natural blood vessel. Smooth muscle cells (SMC) were cultured under pulsatile flow conditions in porous tubular scaffolds composed of crosslinked type I insoluble collagen and insoluble elastin. Under these dynamic culture conditions, average wall shear rate, systolic and diastolic pressures and pressure wave-forms comparable to conditions in the human carotid artery were obtained. Culturing of SMC in tubular scaffolds under dynamic conditions resulted in enhanced tissue formation compared to static conditions. Higher SMC numbers, a more homogeneous distribution of SMC throughout the scaffolds and higher collagen mRNA expression levels were found when cells were cultured under dynamic compared to static conditions. mRNA expression levels of markers of proliferation and apoptosis showed that the higher cell numbers in the scaffolds cultured under dynamic conditions can be explained by increased cell proliferation but not by decreased apoptosis. Glucose consumption and lactate formation by the cells showed that cell metabolism was more aerobic under dynamic compared to static conditions. Lining of the dynamically cultured constructs with a luminal monolayer of endothelial cells might result in vessels suitable for in vivo applications.",
keywords = "Vascular tissue engineering, Elastin, Smooth muscle cells, Collagen, IR-67198, Cell proliferation, Apoptosis, METIS-229842",
author = "P. Engbers-Buijtenhuijs and L. Buttafoco and Poot, {Andreas A.} and Dijkstra, {Pieter J.} and {de Vos}, {Rob A.I.} and Sterk, {Lotus M.Th.} and Geelkerken, {Rob H.} and I. Vermes and Jan Feijen",
year = "2006",
doi = "10.1016/j.biomaterials.2005.10.016",
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volume = "27",
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Engbers-Buijtenhuijs, P, Buttafoco, L, Poot, AA, Dijkstra, PJ, de Vos, RAI, Sterk, LMT, Geelkerken, RH, Vermes, I & Feijen, J 2006, 'Biological characterisation of vascular grafts cultured in a bioreactor' Biomaterials, vol. 27, no. 11, pp. 2390-2397. https://doi.org/10.1016/j.biomaterials.2005.10.016

Biological characterisation of vascular grafts cultured in a bioreactor. / Engbers-Buijtenhuijs, P.; Buttafoco, L.; Poot, Andreas A.; Dijkstra, Pieter J.; de Vos, Rob A.I.; Sterk, Lotus M.Th.; Geelkerken, Rob H.; Vermes, I.; Feijen, Jan.

In: Biomaterials, Vol. 27, No. 11, 2006, p. 2390-2397.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Engbers-Buijtenhuijs, P.

AU - Buttafoco, L.

AU - Poot, Andreas A.

AU - Dijkstra, Pieter J.

AU - de Vos, Rob A.I.

AU - Sterk, Lotus M.Th.

AU - Geelkerken, Rob H.

AU - Vermes, I.

AU - Feijen, Jan

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N2 - In this study, the development is described of a tissue-engineered construct mimicking the structure of a natural blood vessel. Smooth muscle cells (SMC) were cultured under pulsatile flow conditions in porous tubular scaffolds composed of crosslinked type I insoluble collagen and insoluble elastin. Under these dynamic culture conditions, average wall shear rate, systolic and diastolic pressures and pressure wave-forms comparable to conditions in the human carotid artery were obtained. Culturing of SMC in tubular scaffolds under dynamic conditions resulted in enhanced tissue formation compared to static conditions. Higher SMC numbers, a more homogeneous distribution of SMC throughout the scaffolds and higher collagen mRNA expression levels were found when cells were cultured under dynamic compared to static conditions. mRNA expression levels of markers of proliferation and apoptosis showed that the higher cell numbers in the scaffolds cultured under dynamic conditions can be explained by increased cell proliferation but not by decreased apoptosis. Glucose consumption and lactate formation by the cells showed that cell metabolism was more aerobic under dynamic compared to static conditions. Lining of the dynamically cultured constructs with a luminal monolayer of endothelial cells might result in vessels suitable for in vivo applications.

AB - In this study, the development is described of a tissue-engineered construct mimicking the structure of a natural blood vessel. Smooth muscle cells (SMC) were cultured under pulsatile flow conditions in porous tubular scaffolds composed of crosslinked type I insoluble collagen and insoluble elastin. Under these dynamic culture conditions, average wall shear rate, systolic and diastolic pressures and pressure wave-forms comparable to conditions in the human carotid artery were obtained. Culturing of SMC in tubular scaffolds under dynamic conditions resulted in enhanced tissue formation compared to static conditions. Higher SMC numbers, a more homogeneous distribution of SMC throughout the scaffolds and higher collagen mRNA expression levels were found when cells were cultured under dynamic compared to static conditions. mRNA expression levels of markers of proliferation and apoptosis showed that the higher cell numbers in the scaffolds cultured under dynamic conditions can be explained by increased cell proliferation but not by decreased apoptosis. Glucose consumption and lactate formation by the cells showed that cell metabolism was more aerobic under dynamic compared to static conditions. Lining of the dynamically cultured constructs with a luminal monolayer of endothelial cells might result in vessels suitable for in vivo applications.

KW - Vascular tissue engineering

KW - Elastin

KW - Smooth muscle cells

KW - Collagen

KW - IR-67198

KW - Cell proliferation

KW - Apoptosis

KW - METIS-229842

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DO - 10.1016/j.biomaterials.2005.10.016

M3 - Article

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JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 11

ER -