Biomaterial strategies for stem cell maintenance during in vitro expansion

Xiang Zhen Yan, Jeroen J.J.P. Van Den Beucken, Sanne K. Both, Pi Shan Yang, John A. Jansen, Fang Yang*

*Corresponding author for this work

Research output: Contribution to journalReview articleAcademicpeer-review

30 Citations (Scopus)


Stem cells, having the potential for self-renewal and multilineage differentiation, are the building blocks for tissue/organ regeneration. Stem cells can be isolated from various sources but are, in general, available in too small numbers to be used directly for clinical purpose without intermediate expansion procedures in vitro. Although this in vitro expansion of undifferentiated stem cells is necessary, stem cells typically diminish their ability to self-renew and proliferate during passaging. Consequently, maintaining the stemness of stem cells has been recognized as a major challenge in stem cell-based research. This review focuses on the latest developments in maintaining the self-renewal ability of stem cells during in vitro expansion by biomaterial strategies. Further, this review highlights what should be the focus for future studies using stem cells for regenerative applications.

Original languageEnglish
Pages (from-to)340-354
Number of pages15
JournalTissue Engineering - Part B: Reviews
Issue number4
Publication statusPublished - 1 Aug 2014
Externally publishedYes


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