TY - JOUR
T1 - Biomimetic Cascade Network between Interactive Multicompartments Organized by Enzyme-Loaded Silica Nanoreactors
AU - Jo, Seong-Min
AU - Wurm, Frederik R.
AU - Landfester, Katharina
PY - 2018/10/10
Y1 - 2018/10/10
N2 - Physical separation of reactions by interactive multicompartments in biological cells is an attractive motif to design efficient microreactors that create biomimetic cascade reactions. We present an aqueous compartment with three different subcompartments that comprise of silica nanoreactors with encapsulated enzymes, namely, β-glucosidase, glucose oxidase, and peroxidase, providing a model cascade reaction in confinement. The encapsulated enzymes retain their activity as the substrate can reach the active site and the silica shell further protects the enzymes from external stresses, such as heat and proteolytic degradation. We demonstrate the biomimetic cascade reaction in between the compartments (“organelles”) inside of an additional microconfinement (water-in-oil emulsion). This strategy will allow us to design efficient multicompartmentalized reactors for further biological and organic reactions.
AB - Physical separation of reactions by interactive multicompartments in biological cells is an attractive motif to design efficient microreactors that create biomimetic cascade reactions. We present an aqueous compartment with three different subcompartments that comprise of silica nanoreactors with encapsulated enzymes, namely, β-glucosidase, glucose oxidase, and peroxidase, providing a model cascade reaction in confinement. The encapsulated enzymes retain their activity as the substrate can reach the active site and the silica shell further protects the enzymes from external stresses, such as heat and proteolytic degradation. We demonstrate the biomimetic cascade reaction in between the compartments (“organelles”) inside of an additional microconfinement (water-in-oil emulsion). This strategy will allow us to design efficient multicompartmentalized reactors for further biological and organic reactions.
U2 - 10.1021/acsami.8b11198
DO - 10.1021/acsami.8b11198
M3 - Article
SN - 1944-8244
VL - 10
SP - 34230
EP - 34237
JO - ACS applied materials & interfaces
JF - ACS applied materials & interfaces
IS - 40
ER -