Biomimetic coatings vs. collagen sponges as a carrier for BMP-2: A comparison of the osteogenic responses triggered in vivo using an ectopic rat model

Y. Liu, E.B. Hunziker, C. Van de Vaal, K. de Groot

    Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

    7 Citations (Scopus)

    Abstract

    We have developed a new carrier system for bone morphogenetic protein 2 (BMP-2). This agent has been successfully incorporated into biomimetic calcium phosphate implant coatings without losing its osteoinductive potency. Numerous materials have been tested for their efficiency in carrying BMP-2 and in promoting its osteoinductive effects at both ectopic and orthotopic sites. In the present study, we compare the osteoinductive effects of BMP-2 delivered by biomimetic coatings and by classical collagen sponges in vivo using an ectopic rat model. Titanium-alloy discs, either bearing a co-precipitated layer of calcium phosphate and BMP-2 (1.7 μg/disc) or inserted into collagen sponges impregnated with the same drug (10 μg/sponge), were implanted subcutaneously in the dorsal region of rats and the bone-formation process monitored after 2 and 5 weeks. After 2 weeks, the net volume of bone formed in the biomimetic coating group (5.8 mm3/disc) was greater (p < 0.01) than in the collagen sponge one (2.3 mm3/sponge). By the end of the fifth week, the net volume of bone deposited had increased significantly (p < 0.001) in the biomimetic coating group (11.6 mm3/disc), whereas in the collagen sponge one it remained unchanged. (3.0 mm3/sponge; p = 0.82). As a carrier for BMP-2 and in facilitating its osteogenic effects, biomimetic calcium phosphate coatings offer a distinct advantage over the classical collagen sponge.
    Original languageEnglish
    Title of host publicationBioceramics 16
    EditorsMário A. Barbosa, Fernando J. Monteiro, Rui Correia, Betty Leon
    PublisherTrans Tech Publications Ltd
    Pages619-622
    DOIs
    Publication statusPublished - 6 Nov 2004
    Event16th International Symposium on Ceramics in Medicine, Bioceramics 16: Annual Meeting of the International Society for Ceramics in Medicine - Porto, Portugal
    Duration: 6 Nov 20039 Nov 2003
    Conference number: 16

    Publication series

    NameKey Engineering Materials
    PublisherTrans Tech Publications
    Volume254-256
    ISSN (Print)1662-9795
    ISSN (Electronic)1013-9826

    Conference

    Conference16th International Symposium on Ceramics in Medicine, Bioceramics 16
    Abbreviated titleBioceramics
    CountryPortugal
    CityPorto
    Period6/11/039/11/03

    Fingerprint

    Bone Morphogenetic Protein 2
    Biomimetics
    Porifera
    Collagen
    Bone and Bones
    Titanium
    Osteogenesis

    Keywords

    • METIS-237066

    Cite this

    Liu, Y., Hunziker, E. B., Van de Vaal, C., & de Groot, K. (2004). Biomimetic coatings vs. collagen sponges as a carrier for BMP-2: A comparison of the osteogenic responses triggered in vivo using an ectopic rat model. In M. A. Barbosa, F. J. Monteiro, R. Correia, & B. Leon (Eds.), Bioceramics 16 (pp. 619-622). (Key Engineering Materials; Vol. 254-256). Trans Tech Publications Ltd. https://doi.org/10.4028/www.scientific.net/KEM.254-256.619
    Liu, Y. ; Hunziker, E.B. ; Van de Vaal, C. ; de Groot, K. / Biomimetic coatings vs. collagen sponges as a carrier for BMP-2 : A comparison of the osteogenic responses triggered in vivo using an ectopic rat model. Bioceramics 16. editor / Mário A. Barbosa ; Fernando J. Monteiro ; Rui Correia ; Betty Leon. Trans Tech Publications Ltd, 2004. pp. 619-622 (Key Engineering Materials).
    @inproceedings{77882cb0fbcc4370a2e03afdd200af11,
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    abstract = "We have developed a new carrier system for bone morphogenetic protein 2 (BMP-2). This agent has been successfully incorporated into biomimetic calcium phosphate implant coatings without losing its osteoinductive potency. Numerous materials have been tested for their efficiency in carrying BMP-2 and in promoting its osteoinductive effects at both ectopic and orthotopic sites. In the present study, we compare the osteoinductive effects of BMP-2 delivered by biomimetic coatings and by classical collagen sponges in vivo using an ectopic rat model. Titanium-alloy discs, either bearing a co-precipitated layer of calcium phosphate and BMP-2 (1.7 μg/disc) or inserted into collagen sponges impregnated with the same drug (10 μg/sponge), were implanted subcutaneously in the dorsal region of rats and the bone-formation process monitored after 2 and 5 weeks. After 2 weeks, the net volume of bone formed in the biomimetic coating group (5.8 mm3/disc) was greater (p < 0.01) than in the collagen sponge one (2.3 mm3/sponge). By the end of the fifth week, the net volume of bone deposited had increased significantly (p < 0.001) in the biomimetic coating group (11.6 mm3/disc), whereas in the collagen sponge one it remained unchanged. (3.0 mm3/sponge; p = 0.82). As a carrier for BMP-2 and in facilitating its osteogenic effects, biomimetic calcium phosphate coatings offer a distinct advantage over the classical collagen sponge.",
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    Liu, Y, Hunziker, EB, Van de Vaal, C & de Groot, K 2004, Biomimetic coatings vs. collagen sponges as a carrier for BMP-2: A comparison of the osteogenic responses triggered in vivo using an ectopic rat model. in MA Barbosa, FJ Monteiro, R Correia & B Leon (eds), Bioceramics 16. Key Engineering Materials, vol. 254-256, Trans Tech Publications Ltd, pp. 619-622, 16th International Symposium on Ceramics in Medicine, Bioceramics 16, Porto, Portugal, 6/11/03. https://doi.org/10.4028/www.scientific.net/KEM.254-256.619

    Biomimetic coatings vs. collagen sponges as a carrier for BMP-2 : A comparison of the osteogenic responses triggered in vivo using an ectopic rat model. / Liu, Y.; Hunziker, E.B.; Van de Vaal, C.; de Groot, K.

    Bioceramics 16. ed. / Mário A. Barbosa; Fernando J. Monteiro; Rui Correia; Betty Leon. Trans Tech Publications Ltd, 2004. p. 619-622 (Key Engineering Materials; Vol. 254-256).

    Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

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    N2 - We have developed a new carrier system for bone morphogenetic protein 2 (BMP-2). This agent has been successfully incorporated into biomimetic calcium phosphate implant coatings without losing its osteoinductive potency. Numerous materials have been tested for their efficiency in carrying BMP-2 and in promoting its osteoinductive effects at both ectopic and orthotopic sites. In the present study, we compare the osteoinductive effects of BMP-2 delivered by biomimetic coatings and by classical collagen sponges in vivo using an ectopic rat model. Titanium-alloy discs, either bearing a co-precipitated layer of calcium phosphate and BMP-2 (1.7 μg/disc) or inserted into collagen sponges impregnated with the same drug (10 μg/sponge), were implanted subcutaneously in the dorsal region of rats and the bone-formation process monitored after 2 and 5 weeks. After 2 weeks, the net volume of bone formed in the biomimetic coating group (5.8 mm3/disc) was greater (p < 0.01) than in the collagen sponge one (2.3 mm3/sponge). By the end of the fifth week, the net volume of bone deposited had increased significantly (p < 0.001) in the biomimetic coating group (11.6 mm3/disc), whereas in the collagen sponge one it remained unchanged. (3.0 mm3/sponge; p = 0.82). As a carrier for BMP-2 and in facilitating its osteogenic effects, biomimetic calcium phosphate coatings offer a distinct advantage over the classical collagen sponge.

    AB - We have developed a new carrier system for bone morphogenetic protein 2 (BMP-2). This agent has been successfully incorporated into biomimetic calcium phosphate implant coatings without losing its osteoinductive potency. Numerous materials have been tested for their efficiency in carrying BMP-2 and in promoting its osteoinductive effects at both ectopic and orthotopic sites. In the present study, we compare the osteoinductive effects of BMP-2 delivered by biomimetic coatings and by classical collagen sponges in vivo using an ectopic rat model. Titanium-alloy discs, either bearing a co-precipitated layer of calcium phosphate and BMP-2 (1.7 μg/disc) or inserted into collagen sponges impregnated with the same drug (10 μg/sponge), were implanted subcutaneously in the dorsal region of rats and the bone-formation process monitored after 2 and 5 weeks. After 2 weeks, the net volume of bone formed in the biomimetic coating group (5.8 mm3/disc) was greater (p < 0.01) than in the collagen sponge one (2.3 mm3/sponge). By the end of the fifth week, the net volume of bone deposited had increased significantly (p < 0.001) in the biomimetic coating group (11.6 mm3/disc), whereas in the collagen sponge one it remained unchanged. (3.0 mm3/sponge; p = 0.82). As a carrier for BMP-2 and in facilitating its osteogenic effects, biomimetic calcium phosphate coatings offer a distinct advantage over the classical collagen sponge.

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    Liu Y, Hunziker EB, Van de Vaal C, de Groot K. Biomimetic coatings vs. collagen sponges as a carrier for BMP-2: A comparison of the osteogenic responses triggered in vivo using an ectopic rat model. In Barbosa MA, Monteiro FJ, Correia R, Leon B, editors, Bioceramics 16. Trans Tech Publications Ltd. 2004. p. 619-622. (Key Engineering Materials). https://doi.org/10.4028/www.scientific.net/KEM.254-256.619