TY - JOUR
T1 - Biomimetic coprecipitation of calcium phosphate and bovine serum albumin on titanium alloy
AU - Liu, Yuelian
AU - Layrolle, Pierre
AU - de Bruijn, Joost Dick
AU - van Blitterswijk, Clemens
AU - de Groot, K.
PY - 2001
Y1 - 2001
N2 - Titanium alloy implants were precoated biomimetically with a thin and dense layer of calcium phosphate and then incubated either in a supersaturated solution of calcium phosphate or in phosphate-buffered saline, each containing bovine serum albumin (BSA) at various concentrations, under physiological conditions for 48 h. Coated implants then underwent scanning electron microscopy, immunohistochemical evaluation, Fourier transform infrared spectroscopy, and X-ray diffraction. The quantity of BSA taken up by coatings and the kinetics of protein release were monitored colorimetrically. In coatings prepared by the coprecipitation of calcium phosphate and BSA, protein had become incorporated into the mineral crystal latticework. With increasing BSA concentration, matrices decreased in thickness, became more dense, showed lower crystallinity, and underwent a change in crystal geometry. The octacalcium phosphate structure manifested in the absence of protein was gradually transformed into a carbonated apatite form. Preformed mineral coatings became only superficially mantled with a layer of BSA, and the morphology of the mineral matrices themselves remained unchanged. At equivalent protein concentrations, coatings prepared by the coprecipitation of calcium phosphate released only a minute fraction of its protein component under physiological conditions, whereas preformed mineral matrices showed a burst release of their associated protein within a single 2-h period. The biomimetic coating can be a carrier for osteoinductive agents.
AB - Titanium alloy implants were precoated biomimetically with a thin and dense layer of calcium phosphate and then incubated either in a supersaturated solution of calcium phosphate or in phosphate-buffered saline, each containing bovine serum albumin (BSA) at various concentrations, under physiological conditions for 48 h. Coated implants then underwent scanning electron microscopy, immunohistochemical evaluation, Fourier transform infrared spectroscopy, and X-ray diffraction. The quantity of BSA taken up by coatings and the kinetics of protein release were monitored colorimetrically. In coatings prepared by the coprecipitation of calcium phosphate and BSA, protein had become incorporated into the mineral crystal latticework. With increasing BSA concentration, matrices decreased in thickness, became more dense, showed lower crystallinity, and underwent a change in crystal geometry. The octacalcium phosphate structure manifested in the absence of protein was gradually transformed into a carbonated apatite form. Preformed mineral coatings became only superficially mantled with a layer of BSA, and the morphology of the mineral matrices themselves remained unchanged. At equivalent protein concentrations, coatings prepared by the coprecipitation of calcium phosphate released only a minute fraction of its protein component under physiological conditions, whereas preformed mineral matrices showed a burst release of their associated protein within a single 2-h period. The biomimetic coating can be a carrier for osteoinductive agents.
KW - Titanium alloy (Ti6Al4V)
KW - uptake
KW - biomimetic calcium phosphate coating
KW - Release
KW - Protein
KW - IR-71744
U2 - 10.1002/1097-4636(20011205)57:3<327::AID-JBM1175>3.0.CO;2-J
DO - 10.1002/1097-4636(20011205)57:3<327::AID-JBM1175>3.0.CO;2-J
M3 - Article
VL - 57
SP - 327
EP - 335
JO - Journal of biomedical materials research
JF - Journal of biomedical materials research
SN - 0021-9304
IS - 3
ER -