Biomolecular Interaction Monitoring of Autoantibodies by Scanning Surface Plasmon Resonance Microarray Imaging

Angelique M.C. Lokate, J.B. Beusink, G.A.J. Besselink, Ger J.M. Pruijn, Richardus B.M. Schasfoort

Research output: Contribution to journalArticleAcademicpeer-review

72 Citations (Scopus)

Abstract

A new commercial surface plasmon resonance (SPR) imaging analysis system with a novel SPR dip angle scanning principle allows the measurement, without the need for labeling, of the exact SPR dip angle. With this system hundreds of biomolecular interactions can be monitored on microarrays simultaneously and with great precision. The potency of this system is demonstrated by automatically monitoring the interactions between citrullinated peptides and serum autoantibodies of 50 rheumatoid arthritis (RA) patients and 29 controls in a single step. The smallest antibody concentration that could be measured in this experimental setup was 0.5 pM.
Original languageUndefined
Article number10.1021/ja075103x
Pages (from-to)14013-14018
Number of pages6
JournalJournal of the American Chemical Society
Volume129
Issue number7/45
DOIs
Publication statusPublished - 17 Oct 2007

Keywords

  • EWI-11792
  • IR-62134
  • METIS-245967

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