Biomolecular patterning of glass surfaces via strain-promoted cycloaddition of azides and cyclooctynes

M.A. Wijdeven, Carlo Nicosia, A. Borrmann, Jurriaan Huskens, F.L. van Delft

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Metal-free, strain-promoted alkyne–azide cycloaddition (SPAAC) is employed as a versatile technology for the modification of glass with biomolecules. Patterning is executed by stamping of a fluorogenic azidocoumarin or a cyclooctyne to the glass surface, to obtain a unique anchor point for subsequent functionalization by SPAAC. The azidocoumarin at the same time enables straightforward fluorescent read-out of surface reactions. A strong increase in fluorescence is indeed observed upon metal-free reaction with two readily available cyclooctynes, BCN or DIBAC. In addition, functionalized BCN derivatives are employed for glass surface patterning with biotin or even a 27 kDa protein (green fluorescent protein), upon simple incubation.
Original languageUndefined
Pages (from-to)10549-10552
Number of pages4
JournalRSC advances
Issue number21
Publication statusPublished - 2014


  • METIS-308510
  • IR-95101

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