Bioreducible poly(amido amine)s for siRNA delivery

Leonardus Joannes van der Aa

Research output: ThesisPhD Thesis - Research UT, graduation UTAcademic

60 Downloads (Pure)

Abstract

Successes in RNA interference based therapies are still limited due to the lack of efficient delivery of the mediator, small interfering RNA (siRNA), to the targeted site. The key to success can be the delivery of the siRNA molecules by polymer-based carrier systems, since they can be chemically tailored for efficient siRNA encapsulation, protection and intracellular delivery. Recently, bioreducible poly(amido amine)s proved to be a promising class of polymers in the delivery of DNA. In this thesis the development of bioreducible poly(amido amine)s for the intracellular delivery of siRNA is reported. Polymer structure/function relationships on siRNA complexation/decomplexation, cellular delivery, gene silencing efficiency and cytotoxicity were extensively studied in vitro. The results of this thesis show great promise for future research on therapeutic applications with siRNA.
Original languageEnglish
Awarding Institution
  • University of Twente
Supervisors/Advisors
  • Engbersen, J.F.J., Supervisor
  • Schiffelers, Raymond M., Co-Supervisor, External person
Award date1 Jun 2011
Place of PublicationEnschede
Publisher
Print ISBNs978-90--365-3184-9
DOIs
Publication statusPublished - 1 Jun 2011

Fingerprint

Small Interfering RNA
Amines
Polymers
Cytotoxicity
Complexation
Encapsulation
Genes
RNA
Molecules
DNA

Keywords

  • IR-77325
  • METIS-277270

Cite this

van der Aa, L. J. (2011). Bioreducible poly(amido amine)s for siRNA delivery. Enschede: University of Twente. https://doi.org/10.3990/1.9789036531849
van der Aa, Leonardus Joannes. / Bioreducible poly(amido amine)s for siRNA delivery. Enschede : University of Twente, 2011. 208 p.
@phdthesis{82f0efa8a39a4a4f9fd2b52d3b3076a2,
title = "Bioreducible poly(amido amine)s for siRNA delivery",
abstract = "Successes in RNA interference based therapies are still limited due to the lack of efficient delivery of the mediator, small interfering RNA (siRNA), to the targeted site. The key to success can be the delivery of the siRNA molecules by polymer-based carrier systems, since they can be chemically tailored for efficient siRNA encapsulation, protection and intracellular delivery. Recently, bioreducible poly(amido amine)s proved to be a promising class of polymers in the delivery of DNA. In this thesis the development of bioreducible poly(amido amine)s for the intracellular delivery of siRNA is reported. Polymer structure/function relationships on siRNA complexation/decomplexation, cellular delivery, gene silencing efficiency and cytotoxicity were extensively studied in vitro. The results of this thesis show great promise for future research on therapeutic applications with siRNA.",
keywords = "IR-77325, METIS-277270",
author = "{van der Aa}, {Leonardus Joannes}",
year = "2011",
month = "6",
day = "1",
doi = "10.3990/1.9789036531849",
language = "English",
isbn = "978-90--365-3184-9",
publisher = "University of Twente",
address = "Netherlands",
school = "University of Twente",

}

Bioreducible poly(amido amine)s for siRNA delivery. / van der Aa, Leonardus Joannes.

Enschede : University of Twente, 2011. 208 p.

Research output: ThesisPhD Thesis - Research UT, graduation UTAcademic

TY - THES

T1 - Bioreducible poly(amido amine)s for siRNA delivery

AU - van der Aa, Leonardus Joannes

PY - 2011/6/1

Y1 - 2011/6/1

N2 - Successes in RNA interference based therapies are still limited due to the lack of efficient delivery of the mediator, small interfering RNA (siRNA), to the targeted site. The key to success can be the delivery of the siRNA molecules by polymer-based carrier systems, since they can be chemically tailored for efficient siRNA encapsulation, protection and intracellular delivery. Recently, bioreducible poly(amido amine)s proved to be a promising class of polymers in the delivery of DNA. In this thesis the development of bioreducible poly(amido amine)s for the intracellular delivery of siRNA is reported. Polymer structure/function relationships on siRNA complexation/decomplexation, cellular delivery, gene silencing efficiency and cytotoxicity were extensively studied in vitro. The results of this thesis show great promise for future research on therapeutic applications with siRNA.

AB - Successes in RNA interference based therapies are still limited due to the lack of efficient delivery of the mediator, small interfering RNA (siRNA), to the targeted site. The key to success can be the delivery of the siRNA molecules by polymer-based carrier systems, since they can be chemically tailored for efficient siRNA encapsulation, protection and intracellular delivery. Recently, bioreducible poly(amido amine)s proved to be a promising class of polymers in the delivery of DNA. In this thesis the development of bioreducible poly(amido amine)s for the intracellular delivery of siRNA is reported. Polymer structure/function relationships on siRNA complexation/decomplexation, cellular delivery, gene silencing efficiency and cytotoxicity were extensively studied in vitro. The results of this thesis show great promise for future research on therapeutic applications with siRNA.

KW - IR-77325

KW - METIS-277270

U2 - 10.3990/1.9789036531849

DO - 10.3990/1.9789036531849

M3 - PhD Thesis - Research UT, graduation UT

SN - 978-90--365-3184-9

PB - University of Twente

CY - Enschede

ER -

van der Aa LJ. Bioreducible poly(amido amine)s for siRNA delivery. Enschede: University of Twente, 2011. 208 p. https://doi.org/10.3990/1.9789036531849