Bioreducible poly(amido amine)s for siRNA delivery

Leonardus Joannes van der Aa

doi:10.3990/1.9789036531849

Bioreducible poly(amido amine)s for siRNA delivery

Leonardus Joannes van der Aa

Faculty of Science and Technology

Research output: Thesis › PhD Thesis - Research UT, graduation UT

314 Downloads (Pure)

Abstract

Successes in RNA interference based therapies are still limited due to the lack of efficient delivery of the mediator, small interfering RNA (siRNA), to the targeted site. The key to success can be the delivery of the siRNA molecules by polymer-based carrier systems, since they can be chemically tailored for efficient siRNA encapsulation, protection and intracellular delivery. Recently, bioreducible poly(amido amine)s proved to be a promising class of polymers in the delivery of DNA. In this thesis the development of bioreducible poly(amido amine)s for the intracellular delivery of siRNA is reported. Polymer structure/function relationships on siRNA complexation/decomplexation, cellular delivery, gene silencing efficiency and cytotoxicity were extensively studied in vitro. The results of this thesis show great promise for future research on therapeutic applications with siRNA.

Original language	English
Awarding Institution	University of Twente
Supervisors/Advisors	Engbersen, J.F.J., Supervisor Schiffelers, Raymond M., Co-Supervisor, External person
Award date	1 Jun 2011
Place of Publication	Enschede
Publisher	University of Twente
Print ISBNs	978-90--365-3184-9
DOIs	https://doi.org/10.3990/1.9789036531849
Publication status	Published - 1 Jun 2011

Keywords

IR-77325
METIS-277270

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10.3990/1.9789036531849

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title = "Bioreducible poly(amido amine)s for siRNA delivery",

abstract = "Successes in RNA interference based therapies are still limited due to the lack of efficient delivery of the mediator, small interfering RNA (siRNA), to the targeted site. The key to success can be the delivery of the siRNA molecules by polymer-based carrier systems, since they can be chemically tailored for efficient siRNA encapsulation, protection and intracellular delivery. Recently, bioreducible poly(amido amine)s proved to be a promising class of polymers in the delivery of DNA. In this thesis the development of bioreducible poly(amido amine)s for the intracellular delivery of siRNA is reported. Polymer structure/function relationships on siRNA complexation/decomplexation, cellular delivery, gene silencing efficiency and cytotoxicity were extensively studied in vitro. The results of this thesis show great promise for future research on therapeutic applications with siRNA.",

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T1 - Bioreducible poly(amido amine)s for siRNA delivery

AU - van der Aa, Leonardus Joannes

PY - 2011/6/1

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N2 - Successes in RNA interference based therapies are still limited due to the lack of efficient delivery of the mediator, small interfering RNA (siRNA), to the targeted site. The key to success can be the delivery of the siRNA molecules by polymer-based carrier systems, since they can be chemically tailored for efficient siRNA encapsulation, protection and intracellular delivery. Recently, bioreducible poly(amido amine)s proved to be a promising class of polymers in the delivery of DNA. In this thesis the development of bioreducible poly(amido amine)s for the intracellular delivery of siRNA is reported. Polymer structure/function relationships on siRNA complexation/decomplexation, cellular delivery, gene silencing efficiency and cytotoxicity were extensively studied in vitro. The results of this thesis show great promise for future research on therapeutic applications with siRNA.

AB - Successes in RNA interference based therapies are still limited due to the lack of efficient delivery of the mediator, small interfering RNA (siRNA), to the targeted site. The key to success can be the delivery of the siRNA molecules by polymer-based carrier systems, since they can be chemically tailored for efficient siRNA encapsulation, protection and intracellular delivery. Recently, bioreducible poly(amido amine)s proved to be a promising class of polymers in the delivery of DNA. In this thesis the development of bioreducible poly(amido amine)s for the intracellular delivery of siRNA is reported. Polymer structure/function relationships on siRNA complexation/decomplexation, cellular delivery, gene silencing efficiency and cytotoxicity were extensively studied in vitro. The results of this thesis show great promise for future research on therapeutic applications with siRNA.

KW - IR-77325

KW - METIS-277270

U2 - 10.3990/1.9789036531849

DO - 10.3990/1.9789036531849

M3 - PhD Thesis - Research UT, graduation UT

SN - 978-90--365-3184-9

PB - University of Twente

CY - Enschede

ER -

Bioreducible poly(amido amine)s for siRNA delivery

Abstract

Keywords

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