Bioreducible poly(amidoamine)s with charge-reversel properties for intracellular protein delivery

G.M.J.P.C. Coué; Johannes F.J. Engbersen

doi:10.1016/j.jconrel.2010.07.004

Bioreducible poly(amidoamine)s with charge-reversel properties for intracellular protein delivery

G.M.J.P.C. Coué, Johannes F.J. Engbersen

Faculty of Science and Technology

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

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Abstract

An effective intracellular protein delivery system was developed using bioreducible disulfide-containing poly(amidoamine)s with negatively charged citraconic side groups that can give charge-reversal upon pH decrease. These water-soluble and linear polymers efficiently self-assemble with proteins into nanocomplexes by charge attraction. Intracellular-mimicking protein release from the particles is triggered by the reduction of polymer disulfide linkages, causing polymer degradation, and inversion of protein-polymer interaction at endosomal pH due to the charge-reversal of the citraconic side group.

Original language	English
Title of host publication	Proceedings 11th european symposium on controlled drug delivery
Editors	W.E. Hennink, J.F.J. Engbersen
Publisher	Elsevier
Pages	e9-e11
DOIs	https://doi.org/10.1016/j.jconrel.2010.07.004
Publication status	Published - 7 Apr 2010
Event	11th European Symposium on Controlled Drug Delivery, ESCDD 2010 - Hotel Zuiderduin, Egmond aan Zee (NL), Netherlands Duration: 7 Apr 2010 → 9 Apr 2010 Conference number: 11

Publication series

Name	1
Publisher	Elsevier
Number	1
Volume	148
ISSN (Print)	0168-3659
ISSN (Electronic)	1873-4995

Conference

Conference	11th European Symposium on Controlled Drug Delivery, ESCDD 2010
Abbreviated title	ESCDD
Country/Territory	Netherlands
City	Egmond aan Zee (NL)
Period	7/04/10 → 9/04/10

Keywords

METIS-274351
IR-80273

Access to Document

10.1016/j.jconrel.2010.07.004

Cite this

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title = "Bioreducible poly(amidoamine)s with charge-reversel properties for intracellular protein delivery",

abstract = "An effective intracellular protein delivery system was developed using bioreducible disulfide-containing poly(amidoamine)s with negatively charged citraconic side groups that can give charge-reversal upon pH decrease. These water-soluble and linear polymers efficiently self-assemble with proteins into nanocomplexes by charge attraction. Intracellular-mimicking protein release from the particles is triggered by the reduction of polymer disulfide linkages, causing polymer degradation, and inversion of protein-polymer interaction at endosomal pH due to the charge-reversal of the citraconic side group.",

keywords = "METIS-274351, IR-80273",

author = "G.M.J.P.C. Cou{\'e} and Engbersen, {Johannes F.J.}",

year = "2010",

month = apr,

day = "7",

doi = "10.1016/j.jconrel.2010.07.004",

language = "English",

series = "1",

publisher = "Elsevier",

number = "1",

pages = "e9--e11",

editor = "W.E. Hennink and J.F.J. Engbersen",

booktitle = "Proceedings 11th european symposium on controlled drug delivery",

note = "11th European Symposium on Controlled Drug Delivery, ESCDD 2010, ESCDD ; Conference date: 07-04-2010 Through 09-04-2010",

}

Coué, GMJPC & Engbersen, JFJ 2010, Bioreducible poly(amidoamine)s with charge-reversel properties for intracellular protein delivery. in WE Hennink & JFJ Engbersen (eds), Proceedings 11th european symposium on controlled drug delivery. 1, no. 1, vol. 148, Elsevier, pp. e9-e11, 11th European Symposium on Controlled Drug Delivery, ESCDD 2010, Egmond aan Zee (NL), Netherlands, 7/04/10. https://doi.org/10.1016/j.jconrel.2010.07.004

Bioreducible poly(amidoamine)s with charge-reversel properties for intracellular protein delivery. / Coué, G.M.J.P.C.; Engbersen, Johannes F.J.
Proceedings 11th european symposium on controlled drug delivery. ed. / W.E. Hennink; J.F.J. Engbersen. Elsevier, 2010. p. e9-e11 (1; Vol. 148, No. 1).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

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PY - 2010/4/7

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N2 - An effective intracellular protein delivery system was developed using bioreducible disulfide-containing poly(amidoamine)s with negatively charged citraconic side groups that can give charge-reversal upon pH decrease. These water-soluble and linear polymers efficiently self-assemble with proteins into nanocomplexes by charge attraction. Intracellular-mimicking protein release from the particles is triggered by the reduction of polymer disulfide linkages, causing polymer degradation, and inversion of protein-polymer interaction at endosomal pH due to the charge-reversal of the citraconic side group.

AB - An effective intracellular protein delivery system was developed using bioreducible disulfide-containing poly(amidoamine)s with negatively charged citraconic side groups that can give charge-reversal upon pH decrease. These water-soluble and linear polymers efficiently self-assemble with proteins into nanocomplexes by charge attraction. Intracellular-mimicking protein release from the particles is triggered by the reduction of polymer disulfide linkages, causing polymer degradation, and inversion of protein-polymer interaction at endosomal pH due to the charge-reversal of the citraconic side group.

KW - METIS-274351

KW - IR-80273

U2 - 10.1016/j.jconrel.2010.07.004

DO - 10.1016/j.jconrel.2010.07.004

M3 - Conference contribution

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SP - e9-e11

BT - Proceedings 11th european symposium on controlled drug delivery

A2 - Hennink, W.E.

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PB - Elsevier

T2 - 11th European Symposium on Controlled Drug Delivery, ESCDD 2010

Y2 - 7 April 2010 through 9 April 2010

ER -

Bioreducible poly(amidoamine)s with charge-reversel properties for intracellular protein delivery

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Keywords

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