BLM Experimentation and opto-electrical characterization in microchips, towards an integrated platform for drug screening on membrane proteins

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    Abstract

    Experimentation on cell membranes and on membrane proteins commonly makes use of planar and simplified membrane models, or bilayer lipid membranes (BLMs). Although these models are extensively employed, the experimentation is tedious and time-consuming, and mostly limited to electrical measurements. We propose here a dedicated microfluidic platform for BLM experimentation using electrical and optical techniques. BLMs are formed in the closed microdevice by exposing a lipid plug to air and buffer to give a bilayer structure. BLMs are highly stable, reproducible and have an excellent sealing resistance. Single protein studies are shown using α-hemolysin as a protein model.
    Original languageEnglish
    Title of host publication14th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2010
    EditorsSabeth Verpoorte, Helen Andersson-Svahn, Jenny Emnéus, Nicole Pamme
    Place of PublicationSan Diego
    PublisherThe Chemical and Biological Microsystems Society
    Pages830-832
    Number of pages3
    ISBN (Electronic)978-0-9798064-3-8
    ISBN (Print)978-1-6183906-2-2
    Publication statusPublished - 3 Oct 2010
    Event14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, µTAS 2010 - Groningen, Netherlands
    Duration: 3 Oct 20107 Oct 2010
    Conference number: 14

    Publication series

    NameInternational Conference on Miniaterized Systems for Chemistry and Life Sciences : [proceedings]
    PublisherThe Chemical and Biological Microsystems Society
    Volume2010
    ISSN (Print)1556-5904

    Conference

    Conference14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, µTAS 2010
    Abbreviated titleMicroTAS
    CountryNetherlands
    CityGroningen
    Period3/10/107/10/10

    Keywords

    • Microfluidic platform
    • BLM
    • Membrane proteins
    • α-hemolysin

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