Abstract
Experimentation on cell membranes and on membrane proteins commonly makes use of planar and simplified membrane
models, or bilayer lipid membranes (BLMs). Although these models are extensively employed, the experimentation
is tedious and time-consuming, and mostly limited to electrical measurements. We propose here a dedicated microfluidic platform for BLM experimentation using electrical and optical techniques. BLMs are formed in the closed microdevice by exposing a lipid plug to air and buffer to give a bilayer structure. BLMs are highly stable, reproducible and have an excellent sealing resistance. Single protein studies are shown using α-hemolysin as a protein model.
| Original language | English |
|---|---|
| Title of host publication | 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2010 |
| Editors | Sabeth Verpoorte, Helen Andersson-Svahn, Jenny Emnéus, Nicole Pamme |
| Place of Publication | San Diego |
| Publisher | The Chemical and Biological Microsystems Society |
| Pages | 830-832 |
| Number of pages | 3 |
| ISBN (Electronic) | 978-0-9798064-3-8 |
| ISBN (Print) | 978-1-6183906-2-2 |
| Publication status | Published - 3 Oct 2010 |
| Event | 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2010 - Groningen, Netherlands Duration: 3 Oct 2010 → 7 Oct 2010 Conference number: 14 |
Publication series
| Name | International Conference on Miniaterized Systems for Chemistry and Life Sciences : [proceedings] |
|---|---|
| Publisher | The Chemical and Biological Microsystems Society |
| Volume | 2010 |
| ISSN (Print) | 1556-5904 |
Conference
| Conference | 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2010 |
|---|---|
| Abbreviated title | MicroTAS 2010 |
| Country/Territory | Netherlands |
| City | Groningen |
| Period | 3/10/10 → 7/10/10 |
Keywords
- Microfluidic platform
- BLM
- Membrane proteins
- α-hemolysin