BLM Experimentation and opto-electrical characterization in microchips, towards an integrated platform for drug screening on membrane proteins

V.C. Stimberg; I. van Uitert; S. le Gac; A. van den Berg

BLM Experimentation and opto-electrical characterization in microchips, towards an integrated platform for drug screening on membrane proteins

V.C. Stimberg, I. van Uitert, S. le Gac, A. van den Berg

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

1 Citation (Scopus)

6 Downloads (Pure)

Abstract

Experimentation on cell membranes and on membrane proteins commonly makes use of planar and simplified membrane models, or bilayer lipid membranes (BLMs). Although these models are extensively employed, the experimentation is tedious and time-consuming, and mostly limited to electrical measurements. We propose here a dedicated microfluidic platform for BLM experimentation using electrical and optical techniques. BLMs are formed in the closed microdevice by exposing a lipid plug to air and buffer to give a bilayer structure. BLMs are highly stable, reproducible and have an excellent sealing resistance. Single protein studies are shown using α-hemolysin as a protein model.

Original language	English
Title of host publication	14th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2010
Editors	Sabeth Verpoorte, Helen Andersson-Svahn, Jenny Emnéus, Nicole Pamme
Place of Publication	San Diego
Publisher	The Chemical and Biological Microsystems Society
Pages	830-832
Number of pages	3
ISBN (Electronic)	978-0-9798064-3-8
ISBN (Print)	978-1-6183906-2-2
Publication status	Published - 3 Oct 2010
Event	14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, µTAS 2010 - Groningen, Netherlands Duration: 3 Oct 2010 → 7 Oct 2010 Conference number: 14

Publication series

Name	International Conference on Miniaterized Systems for Chemistry and Life Sciences : [proceedings]
Publisher	The Chemical and Biological Microsystems Society
Volume	2010
ISSN (Print)	1556-5904

Conference

Conference	14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, µTAS 2010
Abbreviated title	MicroTAS
Country	Netherlands
City	Groningen
Period	3/10/10 → 7/10/10

Keywords

Microfluidic platform
BLM
Membrane proteins
α-hemolysin

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Stimberg, V. C., van Uitert, I., le Gac, S., & van den Berg, A. (2010). BLM Experimentation and opto-electrical characterization in microchips, towards an integrated platform for drug screening on membrane proteins. In S. Verpoorte, H. Andersson-Svahn, J. Emnéus, & N. Pamme (Eds.), 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2010 (pp. 830-832). (International Conference on Miniaterized Systems for Chemistry and Life Sciences : [proceedings]; Vol. 2010). The Chemical and Biological Microsystems Society.

Stimberg, V.C. ; van Uitert, I. ; le Gac, S. ; van den Berg, A. / BLM Experimentation and opto-electrical characterization in microchips, towards an integrated platform for drug screening on membrane proteins. 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2010. editor / Sabeth Verpoorte ; Helen Andersson-Svahn ; Jenny Emnéus ; Nicole Pamme. San Diego : The Chemical and Biological Microsystems Society, 2010. pp. 830-832 (International Conference on Miniaterized Systems for Chemistry and Life Sciences : [proceedings]).

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title = "BLM Experimentation and opto-electrical characterization in microchips, towards an integrated platform for drug screening on membrane proteins",

abstract = "Experimentation on cell membranes and on membrane proteins commonly makes use of planar and simplified membrane models, or bilayer lipid membranes (BLMs). Although these models are extensively employed, the experimentation is tedious and time-consuming, and mostly limited to electrical measurements. We propose here a dedicated microfluidic platform for BLM experimentation using electrical and optical techniques. BLMs are formed in the closed microdevice by exposing a lipid plug to air and buffer to give a bilayer structure. BLMs are highly stable, reproducible and have an excellent sealing resistance. Single protein studies are shown using α-hemolysin as a protein model.",

keywords = "Microfluidic platform, BLM, Membrane proteins, α-hemolysin",

author = "V.C. Stimberg and {van Uitert}, I. and {le Gac}, S. and {van den Berg}, A.",

year = "2010",

month = oct,

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series = "International Conference on Miniaterized Systems for Chemistry and Life Sciences : [proceedings]",

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editor = "Sabeth Verpoorte and Helen Andersson-Svahn and Jenny Emn{\'e}us and Nicole Pamme",

booktitle = "14th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2010",

note = "14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, µTAS 2010, MicroTAS ; Conference date: 03-10-2010 Through 07-10-2010",

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Stimberg, VC, van Uitert, I, le Gac, S & van den Berg, A 2010, BLM Experimentation and opto-electrical characterization in microchips, towards an integrated platform for drug screening on membrane proteins. in S Verpoorte, H Andersson-Svahn, J Emnéus & N Pamme (eds), 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2010. International Conference on Miniaterized Systems for Chemistry and Life Sciences : [proceedings], vol. 2010, The Chemical and Biological Microsystems Society, San Diego, pp. 830-832, 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, µTAS 2010, Groningen, Netherlands, 3/10/10.

BLM Experimentation and opto-electrical characterization in microchips, towards an integrated platform for drug screening on membrane proteins. / Stimberg, V.C.; van Uitert, I.; le Gac, S.; van den Berg, A.

14th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2010. ed. / Sabeth Verpoorte; Helen Andersson-Svahn; Jenny Emnéus; Nicole Pamme. San Diego : The Chemical and Biological Microsystems Society, 2010. p. 830-832 (International Conference on Miniaterized Systems for Chemistry and Life Sciences : [proceedings]; Vol. 2010).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

TY - GEN

T1 - BLM Experimentation and opto-electrical characterization in microchips, towards an integrated platform for drug screening on membrane proteins

AU - Stimberg, V.C.

AU - van Uitert, I.

AU - le Gac, S.

AU - van den Berg, A.

N1 - Conference code: 14

PY - 2010/10/3

Y1 - 2010/10/3

N2 - Experimentation on cell membranes and on membrane proteins commonly makes use of planar and simplified membrane models, or bilayer lipid membranes (BLMs). Although these models are extensively employed, the experimentation is tedious and time-consuming, and mostly limited to electrical measurements. We propose here a dedicated microfluidic platform for BLM experimentation using electrical and optical techniques. BLMs are formed in the closed microdevice by exposing a lipid plug to air and buffer to give a bilayer structure. BLMs are highly stable, reproducible and have an excellent sealing resistance. Single protein studies are shown using α-hemolysin as a protein model.

AB - Experimentation on cell membranes and on membrane proteins commonly makes use of planar and simplified membrane models, or bilayer lipid membranes (BLMs). Although these models are extensively employed, the experimentation is tedious and time-consuming, and mostly limited to electrical measurements. We propose here a dedicated microfluidic platform for BLM experimentation using electrical and optical techniques. BLMs are formed in the closed microdevice by exposing a lipid plug to air and buffer to give a bilayer structure. BLMs are highly stable, reproducible and have an excellent sealing resistance. Single protein studies are shown using α-hemolysin as a protein model.

KW - Microfluidic platform

KW - BLM

KW - Membrane proteins

KW - α-hemolysin

M3 - Conference contribution

SN - 978-1-6183906-2-2

T3 - International Conference on Miniaterized Systems for Chemistry and Life Sciences : [proceedings]

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BT - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2010

A2 - Verpoorte, Sabeth

A2 - Andersson-Svahn, Helen

A2 - Emnéus, Jenny

A2 - Pamme, Nicole

PB - The Chemical and Biological Microsystems Society

CY - San Diego

T2 - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, µTAS 2010

Y2 - 3 October 2010 through 7 October 2010

ER -

Stimberg VC, van Uitert I, le Gac S , van den Berg A. BLM Experimentation and opto-electrical characterization in microchips, towards an integrated platform for drug screening on membrane proteins. In Verpoorte S, Andersson-Svahn H, Emnéus J, Pamme N, editors, 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2010. San Diego: The Chemical and Biological Microsystems Society. 2010. p. 830-832. (International Conference on Miniaterized Systems for Chemistry and Life Sciences : [proceedings]).