Blood clearance and tissue distribution of pegylated and non-pegylated 6 gold nanorods after intravenous administration in rats

Danielle P.K. Lankveld, R.G. Rayavarapu, Petra Krystek, Agnes G. Oomen, Hennie W. Verharen, Ton van Leeuwen, Wim H. de Jong, Srirang Manohar

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Abstract

Aims: To develop and determine the safety of gold nanorods, whose aspect ratios can be tuned to obtain plasmon peaks between 650 and 850 nm, as contrast enhancing agents for diagnostic and therapeutic applications. Materials & methods: In this study we compared the blood clearance and tissue distribution of cetyl trimethyl ammonium bromide (CTAB)-capped and polyethylene glycol (PEG)-coated gold nanorods after intravenous injection in the tail vein of rats. The gold content in blood and various organs was measured quantitatively with inductively coupled plasma mass spectrometry. Results & discussion: The CTAB-capped gold nanorods were almost immediately (<15 min) cleared from the blood circulation whereas the PEGylation of gold nanorods resulted in a prolonged blood circulation with a half-life time of 19 h and more wide spread tissue distribution. While for the CTAB-capped gold nanorods the tissue distribution was limited to liver, spleen and lung, the PEGylated gold nanorods also distributed to kidney, heart, thymus, brain and testes. PEGylation of the gold nanorods resulted in the spleen being the organ with the highest exposure, whereas for the non-PEGylated CTAB-capped gold nanorods the liver was the organ with the highest exposure, per gram of organ. Conclusion: The PEGylation of gold nanorods resulted in a prolongation of the blood clearance and the highest organ exposure in the spleen. In view of the time frame (up to 48 h) of the observed presence in blood circulation, PEGylated gold nanorods can be considered to be promising candidates for therapeutic and diagnostic imaging purposes
Original languageUndefined
Pages (from-to)339-349
Number of pages11
JournalNanomedicine : nanotechnology, biology and medicine
Volume6
Issue number2
DOIs
Publication statusPublished - 2011

Keywords

  • Gold
  • Nanorods
  • systemic administration
  • IR-80018
  • tissuedistribution
  • METIS-280429
  • blood clearance

Cite this

Lankveld, Danielle P.K. ; Rayavarapu, R.G. ; Krystek, Petra ; Oomen, Agnes G. ; Verharen, Hennie W. ; van Leeuwen, Ton ; de Jong, Wim H. ; Manohar, Srirang. / Blood clearance and tissue distribution of pegylated and non-pegylated 6 gold nanorods after intravenous administration in rats. In: Nanomedicine : nanotechnology, biology and medicine. 2011 ; Vol. 6, No. 2. pp. 339-349.
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title = "Blood clearance and tissue distribution of pegylated and non-pegylated 6 gold nanorods after intravenous administration in rats",
abstract = "Aims: To develop and determine the safety of gold nanorods, whose aspect ratios can be tuned to obtain plasmon peaks between 650 and 850 nm, as contrast enhancing agents for diagnostic and therapeutic applications. Materials & methods: In this study we compared the blood clearance and tissue distribution of cetyl trimethyl ammonium bromide (CTAB)-capped and polyethylene glycol (PEG)-coated gold nanorods after intravenous injection in the tail vein of rats. The gold content in blood and various organs was measured quantitatively with inductively coupled plasma mass spectrometry. Results & discussion: The CTAB-capped gold nanorods were almost immediately (<15 min) cleared from the blood circulation whereas the PEGylation of gold nanorods resulted in a prolonged blood circulation with a half-life time of 19 h and more wide spread tissue distribution. While for the CTAB-capped gold nanorods the tissue distribution was limited to liver, spleen and lung, the PEGylated gold nanorods also distributed to kidney, heart, thymus, brain and testes. PEGylation of the gold nanorods resulted in the spleen being the organ with the highest exposure, whereas for the non-PEGylated CTAB-capped gold nanorods the liver was the organ with the highest exposure, per gram of organ. Conclusion: The PEGylation of gold nanorods resulted in a prolongation of the blood clearance and the highest organ exposure in the spleen. In view of the time frame (up to 48 h) of the observed presence in blood circulation, PEGylated gold nanorods can be considered to be promising candidates for therapeutic and diagnostic imaging purposes",
keywords = "Gold, Nanorods, systemic administration, IR-80018, tissuedistribution, METIS-280429, blood clearance",
author = "Lankveld, {Danielle P.K.} and R.G. Rayavarapu and Petra Krystek and Oomen, {Agnes G.} and Verharen, {Hennie W.} and {van Leeuwen}, Ton and {de Jong}, {Wim H.} and Srirang Manohar",
year = "2011",
doi = "10.2217/nnm.10.122",
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volume = "6",
pages = "339--349",
journal = "Nanomedicine : nanotechnology, biology and medicine",
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Blood clearance and tissue distribution of pegylated and non-pegylated 6 gold nanorods after intravenous administration in rats. / Lankveld, Danielle P.K.; Rayavarapu, R.G.; Krystek, Petra; Oomen, Agnes G.; Verharen, Hennie W.; van Leeuwen, Ton; de Jong, Wim H.; Manohar, Srirang.

In: Nanomedicine : nanotechnology, biology and medicine, Vol. 6, No. 2, 2011, p. 339-349.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Blood clearance and tissue distribution of pegylated and non-pegylated 6 gold nanorods after intravenous administration in rats

AU - Lankveld, Danielle P.K.

AU - Rayavarapu, R.G.

AU - Krystek, Petra

AU - Oomen, Agnes G.

AU - Verharen, Hennie W.

AU - van Leeuwen, Ton

AU - de Jong, Wim H.

AU - Manohar, Srirang

PY - 2011

Y1 - 2011

N2 - Aims: To develop and determine the safety of gold nanorods, whose aspect ratios can be tuned to obtain plasmon peaks between 650 and 850 nm, as contrast enhancing agents for diagnostic and therapeutic applications. Materials & methods: In this study we compared the blood clearance and tissue distribution of cetyl trimethyl ammonium bromide (CTAB)-capped and polyethylene glycol (PEG)-coated gold nanorods after intravenous injection in the tail vein of rats. The gold content in blood and various organs was measured quantitatively with inductively coupled plasma mass spectrometry. Results & discussion: The CTAB-capped gold nanorods were almost immediately (<15 min) cleared from the blood circulation whereas the PEGylation of gold nanorods resulted in a prolonged blood circulation with a half-life time of 19 h and more wide spread tissue distribution. While for the CTAB-capped gold nanorods the tissue distribution was limited to liver, spleen and lung, the PEGylated gold nanorods also distributed to kidney, heart, thymus, brain and testes. PEGylation of the gold nanorods resulted in the spleen being the organ with the highest exposure, whereas for the non-PEGylated CTAB-capped gold nanorods the liver was the organ with the highest exposure, per gram of organ. Conclusion: The PEGylation of gold nanorods resulted in a prolongation of the blood clearance and the highest organ exposure in the spleen. In view of the time frame (up to 48 h) of the observed presence in blood circulation, PEGylated gold nanorods can be considered to be promising candidates for therapeutic and diagnostic imaging purposes

AB - Aims: To develop and determine the safety of gold nanorods, whose aspect ratios can be tuned to obtain plasmon peaks between 650 and 850 nm, as contrast enhancing agents for diagnostic and therapeutic applications. Materials & methods: In this study we compared the blood clearance and tissue distribution of cetyl trimethyl ammonium bromide (CTAB)-capped and polyethylene glycol (PEG)-coated gold nanorods after intravenous injection in the tail vein of rats. The gold content in blood and various organs was measured quantitatively with inductively coupled plasma mass spectrometry. Results & discussion: The CTAB-capped gold nanorods were almost immediately (<15 min) cleared from the blood circulation whereas the PEGylation of gold nanorods resulted in a prolonged blood circulation with a half-life time of 19 h and more wide spread tissue distribution. While for the CTAB-capped gold nanorods the tissue distribution was limited to liver, spleen and lung, the PEGylated gold nanorods also distributed to kidney, heart, thymus, brain and testes. PEGylation of the gold nanorods resulted in the spleen being the organ with the highest exposure, whereas for the non-PEGylated CTAB-capped gold nanorods the liver was the organ with the highest exposure, per gram of organ. Conclusion: The PEGylation of gold nanorods resulted in a prolongation of the blood clearance and the highest organ exposure in the spleen. In view of the time frame (up to 48 h) of the observed presence in blood circulation, PEGylated gold nanorods can be considered to be promising candidates for therapeutic and diagnostic imaging purposes

KW - Gold

KW - Nanorods

KW - systemic administration

KW - IR-80018

KW - tissuedistribution

KW - METIS-280429

KW - blood clearance

U2 - 10.2217/nnm.10.122

DO - 10.2217/nnm.10.122

M3 - Article

VL - 6

SP - 339

EP - 349

JO - Nanomedicine : nanotechnology, biology and medicine

JF - Nanomedicine : nanotechnology, biology and medicine

SN - 1549-9634

IS - 2

ER -