Bone marrow microfluidic devices and methods for preparing and using the same

David Benson Chou (Inventor), Liliana Moreira Teixeira (Inventor), Arianna Rech (Inventor), Richard Novak (Inventor), Donald E. Ingber (Inventor), Yuka Milton (Inventor), Viktoras Frismantas (Inventor), Oren Levy (Inventor)

Research output: Patent

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Abstract

The present disclosure relates to a microfluidic devices and methods for culturing bone marrow cells. Aspects include methods of preparing microfluidic devices and culturing bone marrow cells with the microfluidic devices. In some aspects, a method includes providing a microfluidic device having an upper chamber, a lower chamber, and a porous membrane separating the upper chamber from the lower chamber. The method further includes seeding walls of the lower chamber and a bottom surface of the membrane with endothelial cells. The method further includes providing a matrix within the upper chamber. The matrix includes fibrin gel and bone marrow cells. The method further includes filling or perfusing the upper chamber with a media.

Original languageEnglish
Patent numberUS2020095525
IPCG01N 33/ 50 A I
Priority date26/03/18
Publication statusPublished - 26 Mar 2020
Externally publishedYes

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