Bone marrow stromal cells on a three-dimensional bioactive fiber mesh undergo osteogenic differentiation in the absence of osteogenic media supplements: The effect of silanol groups

Marcia T. Rodrigues, Isabel B. Leonor, N. Groen, Carlos A. Viegas, Isabel R. Dias, Sofia G. Caridade, Joao F. Mano, Manuela E. Gomes, Rui L. Reis

Research output: Contribution to journal › Article › Academic › peer-review

12 Citations (Scopus)

Abstract

Osteogenic differentiation is a tightly regulated process dependent on the stimuli provided by the micro-environment. Silicon-substituted materials are known to have an influence on the osteogenic phenotype of undifferentiated and bone-derived cells. This study aims to investigate the bioactivity profile as well as the mechanical properties of a blend of starch and poly-caprolactone (SPCL) polymeric fiber mesh scaffolds functionalized with silanol (Si–OH) groups as key features for bone tissue engineering strategies. The scaffolds were made from SPCL by a wet spinning technique. A calcium silicate solution was used as a non-solvent to develop an in situ functionalization with Si–OH groups in a single-step approach. We also explored the relevance of silicon incorporated in SPCL–Si scaffolds to the in vitro osteogenic process of goat bone marrow stromal cells (gBMSCs) with and without osteogenic supplements in the culture medium. We hypothesized that SPCL–Si scaffolds could act as physical and chemical millieus to induce per se the osteogenic differentiation of gBMSCs. Results show that osteogenic differentiation of gBMSCs and the production of a mineralized extracellular matrix on bioactive SPCL–Si scaffolds occur for up to 2 weeks, even in the absence of osteogenic supplements in the culture medium. The omission of media supplements to induce osteogenic differentiation is a promising feature towards simplified and cost-effective cell culturing procedures of a potential bioengineered product, and concomitant translation into the clinical field. Thus, the present work demonstrates that SPCL–Si scaffolds and their intrinsic properties sustain gBMSC osteogenic features in vitro, even in the absence of osteogenic supplements to
Original languageUndefined
Pages (from-to)4175-4185
JournalActa biomaterialia
Volume10
Issue number10
DOIs
Publication statusPublished - 2014

Keywords

  • IR-96652
  • METIS-311070

Cite this

Rodrigues, Marcia T. ; Leonor, Isabel B. ; Groen, N. ; Viegas, Carlos A. ; Dias, Isabel R. ; Caridade, Sofia G. ; Mano, Joao F. ; Gomes, Manuela E. ; Reis, Rui L. / Bone marrow stromal cells on a three-dimensional bioactive fiber mesh undergo osteogenic differentiation in the absence of osteogenic media supplements: The effect of silanol groups. In: Acta biomaterialia. 2014 ; Vol. 10, No. 10. pp. 4175-4185.
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abstract = "Osteogenic differentiation is a tightly regulated process dependent on the stimuli provided by the micro-environment. Silicon-substituted materials are known to have an influence on the osteogenic phenotype of undifferentiated and bone-derived cells. This study aims to investigate the bioactivity profile as well as the mechanical properties of a blend of starch and poly-caprolactone (SPCL) polymeric fiber mesh scaffolds functionalized with silanol (Si–OH) groups as key features for bone tissue engineering strategies. The scaffolds were made from SPCL by a wet spinning technique. A calcium silicate solution was used as a non-solvent to develop an in situ functionalization with Si–OH groups in a single-step approach. We also explored the relevance of silicon incorporated in SPCL–Si scaffolds to the in vitro osteogenic process of goat bone marrow stromal cells (gBMSCs) with and without osteogenic supplements in the culture medium. We hypothesized that SPCL–Si scaffolds could act as physical and chemical millieus to induce per se the osteogenic differentiation of gBMSCs. Results show that osteogenic differentiation of gBMSCs and the production of a mineralized extracellular matrix on bioactive SPCL–Si scaffolds occur for up to 2 weeks, even in the absence of osteogenic supplements in the culture medium. The omission of media supplements to induce osteogenic differentiation is a promising feature towards simplified and cost-effective cell culturing procedures of a potential bioengineered product, and concomitant translation into the clinical field. Thus, the present work demonstrates that SPCL–Si scaffolds and their intrinsic properties sustain gBMSC osteogenic features in vitro, even in the absence of osteogenic supplements to",
keywords = "IR-96652, METIS-311070",
author = "Rodrigues, {Marcia T.} and Leonor, {Isabel B.} and N. Groen and Viegas, {Carlos A.} and Dias, {Isabel R.} and Caridade, {Sofia G.} and Mano, {Joao F.} and Gomes, {Manuela E.} and Reis, {Rui L.}",
year = "2014",
doi = "10.1016/j.actbio.2014.05.026",
language = "Undefined",
volume = "10",
pages = "4175--4185",
journal = "Acta biomaterialia",
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Rodrigues, MT, Leonor, IB, Groen, N, Viegas, CA, Dias, IR, Caridade, SG, Mano, JF, Gomes, ME & Reis, RL 2014, 'Bone marrow stromal cells on a three-dimensional bioactive fiber mesh undergo osteogenic differentiation in the absence of osteogenic media supplements: The effect of silanol groups' Acta biomaterialia, vol. 10, no. 10, pp. 4175-4185. https://doi.org/10.1016/j.actbio.2014.05.026

Bone marrow stromal cells on a three-dimensional bioactive fiber mesh undergo osteogenic differentiation in the absence of osteogenic media supplements: The effect of silanol groups. / Rodrigues, Marcia T.; Leonor, Isabel B.; Groen, N.; Viegas, Carlos A.; Dias, Isabel R.; Caridade, Sofia G.; Mano, Joao F.; Gomes, Manuela E.; Reis, Rui L.

In: Acta biomaterialia, Vol. 10, No. 10, 2014, p. 4175-4185.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Bone marrow stromal cells on a three-dimensional bioactive fiber mesh undergo osteogenic differentiation in the absence of osteogenic media supplements: The effect of silanol groups

AU - Rodrigues, Marcia T.

AU - Leonor, Isabel B.

AU - Groen, N.

AU - Viegas, Carlos A.

AU - Dias, Isabel R.

AU - Caridade, Sofia G.

AU - Mano, Joao F.

AU - Gomes, Manuela E.

AU - Reis, Rui L.

PY - 2014

Y1 - 2014

N2 - Osteogenic differentiation is a tightly regulated process dependent on the stimuli provided by the micro-environment. Silicon-substituted materials are known to have an influence on the osteogenic phenotype of undifferentiated and bone-derived cells. This study aims to investigate the bioactivity profile as well as the mechanical properties of a blend of starch and poly-caprolactone (SPCL) polymeric fiber mesh scaffolds functionalized with silanol (Si–OH) groups as key features for bone tissue engineering strategies. The scaffolds were made from SPCL by a wet spinning technique. A calcium silicate solution was used as a non-solvent to develop an in situ functionalization with Si–OH groups in a single-step approach. We also explored the relevance of silicon incorporated in SPCL–Si scaffolds to the in vitro osteogenic process of goat bone marrow stromal cells (gBMSCs) with and without osteogenic supplements in the culture medium. We hypothesized that SPCL–Si scaffolds could act as physical and chemical millieus to induce per se the osteogenic differentiation of gBMSCs. Results show that osteogenic differentiation of gBMSCs and the production of a mineralized extracellular matrix on bioactive SPCL–Si scaffolds occur for up to 2 weeks, even in the absence of osteogenic supplements in the culture medium. The omission of media supplements to induce osteogenic differentiation is a promising feature towards simplified and cost-effective cell culturing procedures of a potential bioengineered product, and concomitant translation into the clinical field. Thus, the present work demonstrates that SPCL–Si scaffolds and their intrinsic properties sustain gBMSC osteogenic features in vitro, even in the absence of osteogenic supplements to

AB - Osteogenic differentiation is a tightly regulated process dependent on the stimuli provided by the micro-environment. Silicon-substituted materials are known to have an influence on the osteogenic phenotype of undifferentiated and bone-derived cells. This study aims to investigate the bioactivity profile as well as the mechanical properties of a blend of starch and poly-caprolactone (SPCL) polymeric fiber mesh scaffolds functionalized with silanol (Si–OH) groups as key features for bone tissue engineering strategies. The scaffolds were made from SPCL by a wet spinning technique. A calcium silicate solution was used as a non-solvent to develop an in situ functionalization with Si–OH groups in a single-step approach. We also explored the relevance of silicon incorporated in SPCL–Si scaffolds to the in vitro osteogenic process of goat bone marrow stromal cells (gBMSCs) with and without osteogenic supplements in the culture medium. We hypothesized that SPCL–Si scaffolds could act as physical and chemical millieus to induce per se the osteogenic differentiation of gBMSCs. Results show that osteogenic differentiation of gBMSCs and the production of a mineralized extracellular matrix on bioactive SPCL–Si scaffolds occur for up to 2 weeks, even in the absence of osteogenic supplements in the culture medium. The omission of media supplements to induce osteogenic differentiation is a promising feature towards simplified and cost-effective cell culturing procedures of a potential bioengineered product, and concomitant translation into the clinical field. Thus, the present work demonstrates that SPCL–Si scaffolds and their intrinsic properties sustain gBMSC osteogenic features in vitro, even in the absence of osteogenic supplements to

KW - IR-96652

KW - METIS-311070

U2 - 10.1016/j.actbio.2014.05.026

DO - 10.1016/j.actbio.2014.05.026

M3 - Article

VL - 10

SP - 4175

EP - 4185

JO - Acta biomaterialia

JF - Acta biomaterialia

SN - 1742-7061

IS - 10

ER -

Rodrigues MT, Leonor IB, Groen N, Viegas CA, Dias IR, Caridade SG et al. Bone marrow stromal cells on a three-dimensional bioactive fiber mesh undergo osteogenic differentiation in the absence of osteogenic media supplements: The effect of silanol groups. Acta biomaterialia. 2014;10(10):4175-4185. https://doi.org/10.1016/j.actbio.2014.05.026

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