Abstract
Original language | Undefined |
---|---|
Pages (from-to) | 4175-4185 |
Journal | Acta biomaterialia |
Volume | 10 |
Issue number | 10 |
DOIs | |
Publication status | Published - 2014 |
Keywords
- IR-96652
- METIS-311070
Cite this
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Bone marrow stromal cells on a three-dimensional bioactive fiber mesh undergo osteogenic differentiation in the absence of osteogenic media supplements: The effect of silanol groups. / Rodrigues, Marcia T.; Leonor, Isabel B.; Groen, N.; Viegas, Carlos A.; Dias, Isabel R.; Caridade, Sofia G.; Mano, Joao F.; Gomes, Manuela E.; Reis, Rui L.
In: Acta biomaterialia, Vol. 10, No. 10, 2014, p. 4175-4185.Research output: Contribution to journal › Article › Academic › peer-review
TY - JOUR
T1 - Bone marrow stromal cells on a three-dimensional bioactive fiber mesh undergo osteogenic differentiation in the absence of osteogenic media supplements: The effect of silanol groups
AU - Rodrigues, Marcia T.
AU - Leonor, Isabel B.
AU - Groen, N.
AU - Viegas, Carlos A.
AU - Dias, Isabel R.
AU - Caridade, Sofia G.
AU - Mano, Joao F.
AU - Gomes, Manuela E.
AU - Reis, Rui L.
PY - 2014
Y1 - 2014
N2 - Osteogenic differentiation is a tightly regulated process dependent on the stimuli provided by the micro-environment. Silicon-substituted materials are known to have an influence on the osteogenic phenotype of undifferentiated and bone-derived cells. This study aims to investigate the bioactivity profile as well as the mechanical properties of a blend of starch and poly-caprolactone (SPCL) polymeric fiber mesh scaffolds functionalized with silanol (Si–OH) groups as key features for bone tissue engineering strategies. The scaffolds were made from SPCL by a wet spinning technique. A calcium silicate solution was used as a non-solvent to develop an in situ functionalization with Si–OH groups in a single-step approach. We also explored the relevance of silicon incorporated in SPCL–Si scaffolds to the in vitro osteogenic process of goat bone marrow stromal cells (gBMSCs) with and without osteogenic supplements in the culture medium. We hypothesized that SPCL–Si scaffolds could act as physical and chemical millieus to induce per se the osteogenic differentiation of gBMSCs. Results show that osteogenic differentiation of gBMSCs and the production of a mineralized extracellular matrix on bioactive SPCL–Si scaffolds occur for up to 2 weeks, even in the absence of osteogenic supplements in the culture medium. The omission of media supplements to induce osteogenic differentiation is a promising feature towards simplified and cost-effective cell culturing procedures of a potential bioengineered product, and concomitant translation into the clinical field. Thus, the present work demonstrates that SPCL–Si scaffolds and their intrinsic properties sustain gBMSC osteogenic features in vitro, even in the absence of osteogenic supplements to
AB - Osteogenic differentiation is a tightly regulated process dependent on the stimuli provided by the micro-environment. Silicon-substituted materials are known to have an influence on the osteogenic phenotype of undifferentiated and bone-derived cells. This study aims to investigate the bioactivity profile as well as the mechanical properties of a blend of starch and poly-caprolactone (SPCL) polymeric fiber mesh scaffolds functionalized with silanol (Si–OH) groups as key features for bone tissue engineering strategies. The scaffolds were made from SPCL by a wet spinning technique. A calcium silicate solution was used as a non-solvent to develop an in situ functionalization with Si–OH groups in a single-step approach. We also explored the relevance of silicon incorporated in SPCL–Si scaffolds to the in vitro osteogenic process of goat bone marrow stromal cells (gBMSCs) with and without osteogenic supplements in the culture medium. We hypothesized that SPCL–Si scaffolds could act as physical and chemical millieus to induce per se the osteogenic differentiation of gBMSCs. Results show that osteogenic differentiation of gBMSCs and the production of a mineralized extracellular matrix on bioactive SPCL–Si scaffolds occur for up to 2 weeks, even in the absence of osteogenic supplements in the culture medium. The omission of media supplements to induce osteogenic differentiation is a promising feature towards simplified and cost-effective cell culturing procedures of a potential bioengineered product, and concomitant translation into the clinical field. Thus, the present work demonstrates that SPCL–Si scaffolds and their intrinsic properties sustain gBMSC osteogenic features in vitro, even in the absence of osteogenic supplements to
KW - IR-96652
KW - METIS-311070
U2 - 10.1016/j.actbio.2014.05.026
DO - 10.1016/j.actbio.2014.05.026
M3 - Article
VL - 10
SP - 4175
EP - 4185
JO - Acta biomaterialia
JF - Acta biomaterialia
SN - 1742-7061
IS - 10
ER -