Bone Tissue-Engineered Implants Using Human Bone Marrow Stromal Cells: Effect of Culture Conditions and Donor Age

S.C. Mendes, J.M. Tibbe, M. Veenhof, K. Bakker, S. Both, P.P. Platenburg, F.C. Oner, J.D. de Bruijn, C>a> van Blitterswijk

Research output: Contribution to journalArticleAcademic

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Abstract

At present, it is well known that populations of human bone marrow stromal cells (HBMSCs) can differentiate into osteoblasts and produce bone. However, the amount of cells with osteogenic potential that is ultimately obtained will still be dependent on both patient physiological status and culture system. In addition, to use a cell therapy approach in orthopedics, large cell numbers will be required and, as a result, knowledge of the factors affecting the growth kinetics of these cells is needed. In the present study we investigated the effect of dexamethasone stimulation on the in vivo osteogenic potential of HBMSCs. After a proliferation step, the cells were seeded and cultured on porous calcium phosphate scaffolds for 1 week, and then subcutaneously implanted in nude mice for 6 weeks, in order to evaluate their in vivo bone-forming ability. Furthermore, the effect of donor age on the proliferation rate of the cultures and their ability to induce in vivo bone formation was studied. In 67% of the assayed patients (8 of 12), the presence of dexamethasone in culture was not required to obtain in vivo bone tissue formation. However, in cultures without bone-forming ability or with a low degree of osteogenesis, dexamethasone increased the bone-forming capacity of the cells. During cellular proliferation, a significant age-related decrease was observed in the growth rate of cells from donors older than 50 years as compared with younger donors. With regard to the effect of donor age on in vivo bone formation, HBMSCs from several donors in all age groups proved to possess in vivo osteogenic potential, indicating that the use of cell therapy in the repair of bone defects can be applicable irrespective of patient age. However, the increase in donor age significantly decreased the frequency of cases in which bone formation was observed.
Original languageEnglish
Pages (from-to)911-920
JournalTissue engineering
Volume8
Issue number6
DOIs
Publication statusPublished - 2002

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Mesenchymal Stromal Cells
Osteogenesis
Cell Culture Techniques
Tissue Donors
Bone and Bones
Dexamethasone
Cell- and Tissue-Based Therapy
Cell Proliferation
Osteoblasts
Nude Mice
Orthopedics
Intercellular Signaling Peptides and Proteins
Age Groups
Cell Count
Growth
Population

Keywords

  • IR-67188

Cite this

Mendes, S. C., Tibbe, J. M., Veenhof, M., Bakker, K., Both, S., Platenburg, P. P., ... van Blitterswijk, C. (2002). Bone Tissue-Engineered Implants Using Human Bone Marrow Stromal Cells: Effect of Culture Conditions and Donor Age. Tissue engineering, 8(6), 911-920. https://doi.org/10.1089/107632702320934010
Mendes, S.C. ; Tibbe, J.M. ; Veenhof, M. ; Bakker, K. ; Both, S. ; Platenburg, P.P. ; Oner, F.C. ; de Bruijn, J.D. ; van Blitterswijk, C>a>. / Bone Tissue-Engineered Implants Using Human Bone Marrow Stromal Cells : Effect of Culture Conditions and Donor Age. In: Tissue engineering. 2002 ; Vol. 8, No. 6. pp. 911-920.
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abstract = "At present, it is well known that populations of human bone marrow stromal cells (HBMSCs) can differentiate into osteoblasts and produce bone. However, the amount of cells with osteogenic potential that is ultimately obtained will still be dependent on both patient physiological status and culture system. In addition, to use a cell therapy approach in orthopedics, large cell numbers will be required and, as a result, knowledge of the factors affecting the growth kinetics of these cells is needed. In the present study we investigated the effect of dexamethasone stimulation on the in vivo osteogenic potential of HBMSCs. After a proliferation step, the cells were seeded and cultured on porous calcium phosphate scaffolds for 1 week, and then subcutaneously implanted in nude mice for 6 weeks, in order to evaluate their in vivo bone-forming ability. Furthermore, the effect of donor age on the proliferation rate of the cultures and their ability to induce in vivo bone formation was studied. In 67{\%} of the assayed patients (8 of 12), the presence of dexamethasone in culture was not required to obtain in vivo bone tissue formation. However, in cultures without bone-forming ability or with a low degree of osteogenesis, dexamethasone increased the bone-forming capacity of the cells. During cellular proliferation, a significant age-related decrease was observed in the growth rate of cells from donors older than 50 years as compared with younger donors. With regard to the effect of donor age on in vivo bone formation, HBMSCs from several donors in all age groups proved to possess in vivo osteogenic potential, indicating that the use of cell therapy in the repair of bone defects can be applicable irrespective of patient age. However, the increase in donor age significantly decreased the frequency of cases in which bone formation was observed.",
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Mendes, SC, Tibbe, JM, Veenhof, M, Bakker, K, Both, S, Platenburg, PP, Oner, FC, de Bruijn, JD & van Blitterswijk, C 2002, 'Bone Tissue-Engineered Implants Using Human Bone Marrow Stromal Cells: Effect of Culture Conditions and Donor Age' Tissue engineering, vol. 8, no. 6, pp. 911-920. https://doi.org/10.1089/107632702320934010

Bone Tissue-Engineered Implants Using Human Bone Marrow Stromal Cells : Effect of Culture Conditions and Donor Age. / Mendes, S.C.; Tibbe, J.M.; Veenhof, M.; Bakker, K.; Both, S.; Platenburg, P.P.; Oner, F.C.; de Bruijn, J.D.; van Blitterswijk, C>a>.

In: Tissue engineering, Vol. 8, No. 6, 2002, p. 911-920.

Research output: Contribution to journalArticleAcademic

TY - JOUR

T1 - Bone Tissue-Engineered Implants Using Human Bone Marrow Stromal Cells

T2 - Effect of Culture Conditions and Donor Age

AU - Mendes, S.C.

AU - Tibbe, J.M.

AU - Veenhof, M.

AU - Bakker, K.

AU - Both, S.

AU - Platenburg, P.P.

AU - Oner, F.C.

AU - de Bruijn, J.D.

AU - van Blitterswijk, C>a>

PY - 2002

Y1 - 2002

N2 - At present, it is well known that populations of human bone marrow stromal cells (HBMSCs) can differentiate into osteoblasts and produce bone. However, the amount of cells with osteogenic potential that is ultimately obtained will still be dependent on both patient physiological status and culture system. In addition, to use a cell therapy approach in orthopedics, large cell numbers will be required and, as a result, knowledge of the factors affecting the growth kinetics of these cells is needed. In the present study we investigated the effect of dexamethasone stimulation on the in vivo osteogenic potential of HBMSCs. After a proliferation step, the cells were seeded and cultured on porous calcium phosphate scaffolds for 1 week, and then subcutaneously implanted in nude mice for 6 weeks, in order to evaluate their in vivo bone-forming ability. Furthermore, the effect of donor age on the proliferation rate of the cultures and their ability to induce in vivo bone formation was studied. In 67% of the assayed patients (8 of 12), the presence of dexamethasone in culture was not required to obtain in vivo bone tissue formation. However, in cultures without bone-forming ability or with a low degree of osteogenesis, dexamethasone increased the bone-forming capacity of the cells. During cellular proliferation, a significant age-related decrease was observed in the growth rate of cells from donors older than 50 years as compared with younger donors. With regard to the effect of donor age on in vivo bone formation, HBMSCs from several donors in all age groups proved to possess in vivo osteogenic potential, indicating that the use of cell therapy in the repair of bone defects can be applicable irrespective of patient age. However, the increase in donor age significantly decreased the frequency of cases in which bone formation was observed.

AB - At present, it is well known that populations of human bone marrow stromal cells (HBMSCs) can differentiate into osteoblasts and produce bone. However, the amount of cells with osteogenic potential that is ultimately obtained will still be dependent on both patient physiological status and culture system. In addition, to use a cell therapy approach in orthopedics, large cell numbers will be required and, as a result, knowledge of the factors affecting the growth kinetics of these cells is needed. In the present study we investigated the effect of dexamethasone stimulation on the in vivo osteogenic potential of HBMSCs. After a proliferation step, the cells were seeded and cultured on porous calcium phosphate scaffolds for 1 week, and then subcutaneously implanted in nude mice for 6 weeks, in order to evaluate their in vivo bone-forming ability. Furthermore, the effect of donor age on the proliferation rate of the cultures and their ability to induce in vivo bone formation was studied. In 67% of the assayed patients (8 of 12), the presence of dexamethasone in culture was not required to obtain in vivo bone tissue formation. However, in cultures without bone-forming ability or with a low degree of osteogenesis, dexamethasone increased the bone-forming capacity of the cells. During cellular proliferation, a significant age-related decrease was observed in the growth rate of cells from donors older than 50 years as compared with younger donors. With regard to the effect of donor age on in vivo bone formation, HBMSCs from several donors in all age groups proved to possess in vivo osteogenic potential, indicating that the use of cell therapy in the repair of bone defects can be applicable irrespective of patient age. However, the increase in donor age significantly decreased the frequency of cases in which bone formation was observed.

KW - IR-67188

U2 - 10.1089/107632702320934010

DO - 10.1089/107632702320934010

M3 - Article

VL - 8

SP - 911

EP - 920

JO - Tissue engineering

JF - Tissue engineering

SN - 1076-3279

IS - 6

ER -