Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 544-551 |
| Number of pages | 8 |
| Journal | Journal of orthopaedic research |
| Volume | 22 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2004 |
Fingerprint
Keywords
- Tissue Engineering
- Orthotopic
- METIS-237056
- IR-76358
- Cells
- Bone
- Goat
- Ectopic
Cite this
}
Bone tissue engineering in a critical size defect compared to ectopic implantations in the goat. / Kruyt, Moyo C.; Dhert, Wouter J.A.; Yuan, Huipin; Wilson, C.E.; van Blitterswijk, Clemens; Verbout, Abraham J.; de Bruijn, Joost Dick.
In: Journal of orthopaedic research, Vol. 22, No. 3, 2004, p. 544-551.Research output: Contribution to journal › Article › Academic › peer-review
TY - JOUR
T1 - Bone tissue engineering in a critical size defect compared to ectopic implantations in the goat
AU - Kruyt, Moyo C.
AU - Dhert, Wouter J.A.
AU - Yuan, Huipin
AU - Wilson, C.E.
AU - van Blitterswijk, Clemens
AU - Verbout, Abraham J.
AU - de Bruijn, Joost Dick
PY - 2004
Y1 - 2004
N2 - Since the application of the autologous bone graft, the need for an alternative has been recognized. Tissue engineering (TE) of bone by combining bone marrow stromal cells (BMSCs) with a porous scaffold, is considered a promising technique. In this study we investigated the potential of tissue engineered bone to heal a critical sized defect in the goat. Orthotopic bone formation was compared to ectopic bone formation in comparable constructs. TE constructs were prepared from goat BMSCs and porous biphasic calcium phosphate ceramic scaffolds. These constructs and scaffolds without cells were implanted paired in critical sized iliac wing defects. Comparable samples were implanted intramuscularly. After 9 (n=7) and 12 (n=8) weeks implantation, the samples were analyzed histomorphometrically. After 9-weeks implantation in the iliac wing defect, significantly more bone apposition was found in the TE condition. After 12 weeks, the defects were almost completely filled with bone, but no significant advantage of TE was determined anymore. This contrasted with the intramuscular samples where TE implants showed significantly more bone at both time points. In conclusion, bone TE is feasible in critical sized defects. However, when appropriate osteoconductive/inductive materials are applied the effect of cell seeding may be temporary.
AB - Since the application of the autologous bone graft, the need for an alternative has been recognized. Tissue engineering (TE) of bone by combining bone marrow stromal cells (BMSCs) with a porous scaffold, is considered a promising technique. In this study we investigated the potential of tissue engineered bone to heal a critical sized defect in the goat. Orthotopic bone formation was compared to ectopic bone formation in comparable constructs. TE constructs were prepared from goat BMSCs and porous biphasic calcium phosphate ceramic scaffolds. These constructs and scaffolds without cells were implanted paired in critical sized iliac wing defects. Comparable samples were implanted intramuscularly. After 9 (n=7) and 12 (n=8) weeks implantation, the samples were analyzed histomorphometrically. After 9-weeks implantation in the iliac wing defect, significantly more bone apposition was found in the TE condition. After 12 weeks, the defects were almost completely filled with bone, but no significant advantage of TE was determined anymore. This contrasted with the intramuscular samples where TE implants showed significantly more bone at both time points. In conclusion, bone TE is feasible in critical sized defects. However, when appropriate osteoconductive/inductive materials are applied the effect of cell seeding may be temporary.
KW - Tissue Engineering
KW - Orthotopic
KW - METIS-237056
KW - IR-76358
KW - Cells
KW - Bone
KW - Goat
KW - Ectopic
U2 - 10.1016/j.orthres.2003.10.010
DO - 10.1016/j.orthres.2003.10.010
M3 - Article
VL - 22
SP - 544
EP - 551
JO - Journal of orthopaedic research
JF - Journal of orthopaedic research
SN - 0736-0266
IS - 3
ER -