Bone tissue engineering in a critical size defect compared to ectopic implantations in the goat

Moyo C. Kruyt, Wouter J.A. Dhert, Huipin Yuan, C.E. Wilson, Clemens van Blitterswijk, Abraham J. Verbout, Joost Dick de Bruijn

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Abstract

Since the application of the autologous bone graft, the need for an alternative has been recognized. Tissue engineering (TE) of bone by combining bone marrow stromal cells (BMSCs) with a porous scaffold, is considered a promising technique. In this study we investigated the potential of tissue engineered bone to heal a critical sized defect in the goat. Orthotopic bone formation was compared to ectopic bone formation in comparable constructs. TE constructs were prepared from goat BMSCs and porous biphasic calcium phosphate ceramic scaffolds. These constructs and scaffolds without cells were implanted paired in critical sized iliac wing defects. Comparable samples were implanted intramuscularly. After 9 (n=7) and 12 (n=8) weeks implantation, the samples were analyzed histomorphometrically. After 9-weeks implantation in the iliac wing defect, significantly more bone apposition was found in the TE condition. After 12 weeks, the defects were almost completely filled with bone, but no significant advantage of TE was determined anymore. This contrasted with the intramuscular samples where TE implants showed significantly more bone at both time points. In conclusion, bone TE is feasible in critical sized defects. However, when appropriate osteoconductive/inductive materials are applied the effect of cell seeding may be temporary.
Original languageEnglish
Pages (from-to)544-551
Number of pages8
JournalJournal of orthopaedic research
Volume22
Issue number3
DOIs
Publication statusPublished - 2004

Fingerprint

Tissue Engineering
Goats
Bone and Bones
Mesenchymal Stromal Cells
Osteogenesis
Ceramics
Transplants

Keywords

  • Tissue Engineering
  • Orthotopic
  • METIS-237056
  • IR-76358
  • Cells
  • Bone
  • Goat
  • Ectopic

Cite this

Kruyt, M. C., Dhert, W. J. A., Yuan, H., Wilson, C. E., van Blitterswijk, C., Verbout, A. J., & de Bruijn, J. D. (2004). Bone tissue engineering in a critical size defect compared to ectopic implantations in the goat. Journal of orthopaedic research, 22(3), 544-551. https://doi.org/10.1016/j.orthres.2003.10.010
Kruyt, Moyo C. ; Dhert, Wouter J.A. ; Yuan, Huipin ; Wilson, C.E. ; van Blitterswijk, Clemens ; Verbout, Abraham J. ; de Bruijn, Joost Dick. / Bone tissue engineering in a critical size defect compared to ectopic implantations in the goat. In: Journal of orthopaedic research. 2004 ; Vol. 22, No. 3. pp. 544-551.
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Kruyt, MC, Dhert, WJA, Yuan, H, Wilson, CE, van Blitterswijk, C, Verbout, AJ & de Bruijn, JD 2004, 'Bone tissue engineering in a critical size defect compared to ectopic implantations in the goat' Journal of orthopaedic research, vol. 22, no. 3, pp. 544-551. https://doi.org/10.1016/j.orthres.2003.10.010

Bone tissue engineering in a critical size defect compared to ectopic implantations in the goat. / Kruyt, Moyo C.; Dhert, Wouter J.A.; Yuan, Huipin; Wilson, C.E.; van Blitterswijk, Clemens; Verbout, Abraham J.; de Bruijn, Joost Dick.

In: Journal of orthopaedic research, Vol. 22, No. 3, 2004, p. 544-551.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - Bone tissue engineering in a critical size defect compared to ectopic implantations in the goat

AU - Kruyt, Moyo C.

AU - Dhert, Wouter J.A.

AU - Yuan, Huipin

AU - Wilson, C.E.

AU - van Blitterswijk, Clemens

AU - Verbout, Abraham J.

AU - de Bruijn, Joost Dick

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AB - Since the application of the autologous bone graft, the need for an alternative has been recognized. Tissue engineering (TE) of bone by combining bone marrow stromal cells (BMSCs) with a porous scaffold, is considered a promising technique. In this study we investigated the potential of tissue engineered bone to heal a critical sized defect in the goat. Orthotopic bone formation was compared to ectopic bone formation in comparable constructs. TE constructs were prepared from goat BMSCs and porous biphasic calcium phosphate ceramic scaffolds. These constructs and scaffolds without cells were implanted paired in critical sized iliac wing defects. Comparable samples were implanted intramuscularly. After 9 (n=7) and 12 (n=8) weeks implantation, the samples were analyzed histomorphometrically. After 9-weeks implantation in the iliac wing defect, significantly more bone apposition was found in the TE condition. After 12 weeks, the defects were almost completely filled with bone, but no significant advantage of TE was determined anymore. This contrasted with the intramuscular samples where TE implants showed significantly more bone at both time points. In conclusion, bone TE is feasible in critical sized defects. However, when appropriate osteoconductive/inductive materials are applied the effect of cell seeding may be temporary.

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JO - Journal of orthopaedic research

JF - Journal of orthopaedic research

SN - 0736-0266

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