Both Poly(ethylene glycol) and Poly(methyl ethylene phosphate) Guide Oriented Adsorption of Specific Proteins

Christoph Bernhard; Steven J. Roeters; Kristin N. Bauer; Tobias Weidner; Mischa Bonn; Frederik R. Wurm; Grazia Gonella

doi:10.1021/acs.langmuir.9b02275

Both Poly(ethylene glycol) and Poly(methyl ethylene phosphate) Guide Oriented Adsorption of Specific Proteins

Christoph Bernhard, Steven J. Roeters, Kristin N. Bauer, Tobias Weidner, Mischa Bonn, Frederik R. Wurm, Grazia Gonella^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

3 Citations (Scopus)

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Abstract

Developing new functional biomaterials requires the ability to simultaneously repel unwanted and guide wanted protein adsorption. Here, we systematically interrogate the factors determining the protein adsorption by comparing the behaviors of different polymeric surfaces, poly(ethylene glycol) and a poly(phosphoester), and five different natural proteins. Interestingly we observe that, at densities comparable to those used in nanocarrier functionalization, the same proteins are either adsorbed (fibrinogen, human serum albumin, and transferrin) or repelled (immunoglobulin G and lysozyme) by both polymers. However, when adsorption takes place, the specific surface dictates the amount and orientation of each protein.

Original language	English
Pages (from-to)	14092–14097
Journal	Langmuir
Volume	35
Issue number	43
DOIs	https://doi.org/10.1021/acs.langmuir.9b02275
Publication status	Published - 29 Oct 2019
Externally published	Yes

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Bernhard-2019-Both-polyethylene-glycol-and-polymeFinal published version, 1.25 MBLicence: CC BY

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title = "Both Poly(ethylene glycol) and Poly(methyl ethylene phosphate) Guide Oriented Adsorption of Specific Proteins",

abstract = "Developing new functional biomaterials requires the ability to simultaneously repel unwanted and guide wanted protein adsorption. Here, we systematically interrogate the factors determining the protein adsorption by comparing the behaviors of different polymeric surfaces, poly(ethylene glycol) and a poly(phosphoester), and five different natural proteins. Interestingly we observe that, at densities comparable to those used in nanocarrier functionalization, the same proteins are either adsorbed (fibrinogen, human serum albumin, and transferrin) or repelled (immunoglobulin G and lysozyme) by both polymers. However, when adsorption takes place, the specific surface dictates the amount and orientation of each protein.",

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AU - Bernhard, Christoph

AU - Roeters, Steven J.

AU - Bauer, Kristin N.

AU - Weidner, Tobias

AU - Bonn, Mischa

AU - Wurm, Frederik R.

AU - Gonella, Grazia

PY - 2019/10/29

Y1 - 2019/10/29

N2 - Developing new functional biomaterials requires the ability to simultaneously repel unwanted and guide wanted protein adsorption. Here, we systematically interrogate the factors determining the protein adsorption by comparing the behaviors of different polymeric surfaces, poly(ethylene glycol) and a poly(phosphoester), and five different natural proteins. Interestingly we observe that, at densities comparable to those used in nanocarrier functionalization, the same proteins are either adsorbed (fibrinogen, human serum albumin, and transferrin) or repelled (immunoglobulin G and lysozyme) by both polymers. However, when adsorption takes place, the specific surface dictates the amount and orientation of each protein.

AB - Developing new functional biomaterials requires the ability to simultaneously repel unwanted and guide wanted protein adsorption. Here, we systematically interrogate the factors determining the protein adsorption by comparing the behaviors of different polymeric surfaces, poly(ethylene glycol) and a poly(phosphoester), and five different natural proteins. Interestingly we observe that, at densities comparable to those used in nanocarrier functionalization, the same proteins are either adsorbed (fibrinogen, human serum albumin, and transferrin) or repelled (immunoglobulin G and lysozyme) by both polymers. However, when adsorption takes place, the specific surface dictates the amount and orientation of each protein.

U2 - 10.1021/acs.langmuir.9b02275

DO - 10.1021/acs.langmuir.9b02275

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JO - Langmuir

JF - Langmuir

SN - 0743-7463

IS - 43

ER -

Both Poly(ethylene glycol) and Poly(methyl ethylene phosphate) Guide Oriented Adsorption of Specific Proteins

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