Abstract
Adipose tissues hold great promise in bone tissue engineering since they are available in large quantities as a waste material. The buccal fat
pad (BFP) is a specialized mass of adipose tissue that can be easily obtained via the oral cavity without injury to the external body surface.
Another advantage of BFP over subcutaneous fat is that its size appears to be similar among different people, independent of body weight and
fat distribution. However, limited studies have been conducted on the osteogenic capability of stem cells derived from BFP (BFPSCs).
In this study, the BFPSCs were characterized for their osteogenic differentiation potential especially in contact with a synthetic scaffold in a
perfusion bioreactor. The features of BFPSCs were compared with bone marrow-derived stem cells (BMSCs) as a well known cell source for
bone tissue engineering.
Comparing BFPSCs with BMSCs indicated similar morphology, but faster proliferation rate of BMSCs. Moreover, when properly induced for two
weeks, BFPSCs resembled BMSCs in the production of bone-specific markers, such as alkaline phosphatase, collagen, bone morphogenic
protein (BMP), Runx2, and osteocalcin. Both cell types attached nicely to the pores of a gelatin-coated β-Tricalcium phosphate scaffolds. More
osteogenic differentiation potential was observed for both cells under dynamic culture in a perfusion bioreactor compared with static culture.
The highest collagen content and BMP production were observed in BFPSCs cultured in the bioreactor for two weeks. These results define BFP
as a new, rich, and accessible source of stem cells for tissue engineering purposes.
pad (BFP) is a specialized mass of adipose tissue that can be easily obtained via the oral cavity without injury to the external body surface.
Another advantage of BFP over subcutaneous fat is that its size appears to be similar among different people, independent of body weight and
fat distribution. However, limited studies have been conducted on the osteogenic capability of stem cells derived from BFP (BFPSCs).
In this study, the BFPSCs were characterized for their osteogenic differentiation potential especially in contact with a synthetic scaffold in a
perfusion bioreactor. The features of BFPSCs were compared with bone marrow-derived stem cells (BMSCs) as a well known cell source for
bone tissue engineering.
Comparing BFPSCs with BMSCs indicated similar morphology, but faster proliferation rate of BMSCs. Moreover, when properly induced for two
weeks, BFPSCs resembled BMSCs in the production of bone-specific markers, such as alkaline phosphatase, collagen, bone morphogenic
protein (BMP), Runx2, and osteocalcin. Both cell types attached nicely to the pores of a gelatin-coated β-Tricalcium phosphate scaffolds. More
osteogenic differentiation potential was observed for both cells under dynamic culture in a perfusion bioreactor compared with static culture.
The highest collagen content and BMP production were observed in BFPSCs cultured in the bioreactor for two weeks. These results define BFP
as a new, rich, and accessible source of stem cells for tissue engineering purposes.
| Original language | English |
|---|---|
| Pages | 613 |
| Number of pages | 1 |
| Publication status | Published - 7 Sept 2018 |
| Event | 5th TERMIS World Congress 2018: Integration of Industry, Government, and Academia for Regenerative Medicine - Kyoto International Conference Center , Kyoto, Japan Duration: 4 Sept 2018 → 7 Sept 2018 Conference number: 5 https://www.termis.org/wc2018/ |
Conference
| Conference | 5th TERMIS World Congress 2018 |
|---|---|
| Abbreviated title | TERMIS-WC |
| Country/Territory | Japan |
| City | Kyoto |
| Period | 4/09/18 → 7/09/18 |
| Internet address |