Buccal fat pad as a potential source of stem cells for bone regeneration: an in vitro study

Nasim Salehi Nik, Nazanin Ghasemi (Contributor), Jeroen Rouwkema, Arash Khojasteh

    Research output: Contribution to conferenceAbstract

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    Abstract

    Adipose tissues hold great promise in bone tissue engineering since they are available in large quantities as a waste material. The buccal fat
    pad (BFP) is a specialized mass of adipose tissue that can be easily obtained via the oral cavity without injury to the external body surface.
    Another advantage of BFP over subcutaneous fat is that its size appears to be similar among different people, independent of body weight and
    fat distribution. However, limited studies have been conducted on the osteogenic capability of stem cells derived from BFP (BFPSCs).
    In this study, the BFPSCs were characterized for their osteogenic differentiation potential especially in contact with a synthetic scaffold in a
    perfusion bioreactor. The features of BFPSCs were compared with bone marrow-derived stem cells (BMSCs) as a well known cell source for
    bone tissue engineering.
    Comparing BFPSCs with BMSCs indicated similar morphology, but faster proliferation rate of BMSCs. Moreover, when properly induced for two
    weeks, BFPSCs resembled BMSCs in the production of bone-specific markers, such as alkaline phosphatase, collagen, bone morphogenic
    protein (BMP), Runx2, and osteocalcin. Both cell types attached nicely to the pores of a gelatin-coated β-Tricalcium phosphate scaffolds. More
    osteogenic differentiation potential was observed for both cells under dynamic culture in a perfusion bioreactor compared with static culture.
    The highest collagen content and BMP production were observed in BFPSCs cultured in the bioreactor for two weeks. These results define BFP
    as a new, rich, and accessible source of stem cells for tissue engineering purposes.
    Original languageEnglish
    Pages613
    Number of pages1
    Publication statusPublished - 7 Sep 2018
    Event5th TERMIS World Congress 2018: Integration of Industry, Government, and Academia for Regenerative Medicine - Kyoto International Conference Center , Kyoto, Japan
    Duration: 4 Sep 20187 Sep 2018
    Conference number: 5
    https://www.termis.org/wc2018/

    Conference

    Conference5th TERMIS World Congress 2018
    Abbreviated titleTERMIS-WC
    CountryJapan
    CityKyoto
    Period4/09/187/09/18
    Internet address

    Fingerprint

    Bone Regeneration
    Cheek
    Adipose Tissue
    Stem Cells
    Bioreactors
    Tissue Engineering
    Bone Marrow
    Bone and Bones
    Collagen
    Cell Engineering
    Subcutaneous Fat
    Osteocalcin
    Gelatin
    Alkaline Phosphatase
    Mouth
    In Vitro Techniques
    Perfusion
    Body Weight
    Wounds and Injuries

    Cite this

    Salehi Nik, N., Ghasemi, N., Rouwkema, J., & Khojasteh, A. (2018). Buccal fat pad as a potential source of stem cells for bone regeneration: an in vitro study. 613. Abstract from 5th TERMIS World Congress 2018, Kyoto, Japan.
    Salehi Nik, Nasim ; Ghasemi, Nazanin ; Rouwkema, Jeroen ; Khojasteh, Arash. / Buccal fat pad as a potential source of stem cells for bone regeneration : an in vitro study. Abstract from 5th TERMIS World Congress 2018, Kyoto, Japan.1 p.
    @conference{9a32e4bfc4fe425dacdb1d5ff1ce616c,
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    abstract = "Adipose tissues hold great promise in bone tissue engineering since they are available in large quantities as a waste material. The buccal fatpad (BFP) is a specialized mass of adipose tissue that can be easily obtained via the oral cavity without injury to the external body surface.Another advantage of BFP over subcutaneous fat is that its size appears to be similar among different people, independent of body weight andfat distribution. However, limited studies have been conducted on the osteogenic capability of stem cells derived from BFP (BFPSCs). In this study, the BFPSCs were characterized for their osteogenic differentiation potential especially in contact with a synthetic scaffold in aperfusion bioreactor. The features of BFPSCs were compared with bone marrow-derived stem cells (BMSCs) as a well known cell source forbone tissue engineering. Comparing BFPSCs with BMSCs indicated similar morphology, but faster proliferation rate of BMSCs. Moreover, when properly induced for twoweeks, BFPSCs resembled BMSCs in the production of bone-specific markers, such as alkaline phosphatase, collagen, bone morphogenicprotein (BMP), Runx2, and osteocalcin. Both cell types attached nicely to the pores of a gelatin-coated β-Tricalcium phosphate scaffolds. Moreosteogenic differentiation potential was observed for both cells under dynamic culture in a perfusion bioreactor compared with static culture.The highest collagen content and BMP production were observed in BFPSCs cultured in the bioreactor for two weeks. These results define BFPas a new, rich, and accessible source of stem cells for tissue engineering purposes.",
    author = "{Salehi Nik}, Nasim and Nazanin Ghasemi and Jeroen Rouwkema and Arash Khojasteh",
    note = "abstract a92207, 03-P092; 5th TERMIS World Congress 2018 : Integration of Industry, Government, and Academia for Regenerative Medicine, TERMIS-WC ; Conference date: 04-09-2018 Through 07-09-2018",
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    Salehi Nik, N, Ghasemi, N, Rouwkema, J & Khojasteh, A 2018, 'Buccal fat pad as a potential source of stem cells for bone regeneration: an in vitro study' 5th TERMIS World Congress 2018, Kyoto, Japan, 4/09/18 - 7/09/18, pp. 613.

    Buccal fat pad as a potential source of stem cells for bone regeneration : an in vitro study. / Salehi Nik, Nasim; Ghasemi, Nazanin (Contributor); Rouwkema, Jeroen; Khojasteh, Arash.

    2018. 613 Abstract from 5th TERMIS World Congress 2018, Kyoto, Japan.

    Research output: Contribution to conferenceAbstract

    TY - CONF

    T1 - Buccal fat pad as a potential source of stem cells for bone regeneration

    T2 - an in vitro study

    AU - Salehi Nik, Nasim

    AU - Rouwkema, Jeroen

    AU - Khojasteh, Arash

    A2 - Ghasemi, Nazanin

    N1 - abstract a92207, 03-P092

    PY - 2018/9/7

    Y1 - 2018/9/7

    N2 - Adipose tissues hold great promise in bone tissue engineering since they are available in large quantities as a waste material. The buccal fatpad (BFP) is a specialized mass of adipose tissue that can be easily obtained via the oral cavity without injury to the external body surface.Another advantage of BFP over subcutaneous fat is that its size appears to be similar among different people, independent of body weight andfat distribution. However, limited studies have been conducted on the osteogenic capability of stem cells derived from BFP (BFPSCs). In this study, the BFPSCs were characterized for their osteogenic differentiation potential especially in contact with a synthetic scaffold in aperfusion bioreactor. The features of BFPSCs were compared with bone marrow-derived stem cells (BMSCs) as a well known cell source forbone tissue engineering. Comparing BFPSCs with BMSCs indicated similar morphology, but faster proliferation rate of BMSCs. Moreover, when properly induced for twoweeks, BFPSCs resembled BMSCs in the production of bone-specific markers, such as alkaline phosphatase, collagen, bone morphogenicprotein (BMP), Runx2, and osteocalcin. Both cell types attached nicely to the pores of a gelatin-coated β-Tricalcium phosphate scaffolds. Moreosteogenic differentiation potential was observed for both cells under dynamic culture in a perfusion bioreactor compared with static culture.The highest collagen content and BMP production were observed in BFPSCs cultured in the bioreactor for two weeks. These results define BFPas a new, rich, and accessible source of stem cells for tissue engineering purposes.

    AB - Adipose tissues hold great promise in bone tissue engineering since they are available in large quantities as a waste material. The buccal fatpad (BFP) is a specialized mass of adipose tissue that can be easily obtained via the oral cavity without injury to the external body surface.Another advantage of BFP over subcutaneous fat is that its size appears to be similar among different people, independent of body weight andfat distribution. However, limited studies have been conducted on the osteogenic capability of stem cells derived from BFP (BFPSCs). In this study, the BFPSCs were characterized for their osteogenic differentiation potential especially in contact with a synthetic scaffold in aperfusion bioreactor. The features of BFPSCs were compared with bone marrow-derived stem cells (BMSCs) as a well known cell source forbone tissue engineering. Comparing BFPSCs with BMSCs indicated similar morphology, but faster proliferation rate of BMSCs. Moreover, when properly induced for twoweeks, BFPSCs resembled BMSCs in the production of bone-specific markers, such as alkaline phosphatase, collagen, bone morphogenicprotein (BMP), Runx2, and osteocalcin. Both cell types attached nicely to the pores of a gelatin-coated β-Tricalcium phosphate scaffolds. Moreosteogenic differentiation potential was observed for both cells under dynamic culture in a perfusion bioreactor compared with static culture.The highest collagen content and BMP production were observed in BFPSCs cultured in the bioreactor for two weeks. These results define BFPas a new, rich, and accessible source of stem cells for tissue engineering purposes.

    M3 - Abstract

    SP - 613

    ER -

    Salehi Nik N, Ghasemi N, Rouwkema J, Khojasteh A. Buccal fat pad as a potential source of stem cells for bone regeneration: an in vitro study. 2018. Abstract from 5th TERMIS World Congress 2018, Kyoto, Japan.