Calcification state of algae studied with impedance flow cytometry

Douwe Sietse de Bruijn; Paulus Martinus ter Braak; Dedmer B. Van de Waal; Johan G. Bomer; Wouter Olthuis; Albert van den Berg

Calcification state of algae studied with impedance flow cytometry

Douwe Sietse de Bruijn^*, Paulus Martinus ter Braak, Dedmer B. Van de Waal, Johan G. Bomer, Wouter Olthuis, Albert van den Berg

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

2 Citations (Scopus)

123 Downloads (Pure)

Abstract

The formation of calcite exoskeletons by calcifying algae plays an important role in the ocean carbon cycle. In this study we determine the average calcification state of coccolithophores based on their dielectric properties using impedance flow cytometry. This will enable us to study the effect of environmental factors on single cell calcification in a wide range of coccolithophore genotypes.

Original language	English
Title of host publication	MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Subtitle of host publication	10 - 14 October 2021, Palm Springs, CA, USA
Publisher	The Chemical and Biological Microsystems Society
Pages	559-560
Number of pages	2
ISBN (Electronic)	9781733419031
Publication status	Published - 11 Oct 2021
Event	25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2021 - Palm Springs, United States Duration: 10 Oct 2021 → 14 Oct 2021 Conference number: 25 https://microtas2021.org/

Conference

Conference	25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2021
Abbreviated title	MicroTAS 2021
Country/Territory	United States
City	Palm Springs
Period	10/10/21 → 14/10/21
Internet address	https://microtas2021.org/

Keywords

Calcifying algae
Impedance flow cytometry
Microfluidics
Single-cell analysis

Access to Document

PG0559Final published version, 937 KB

Cite this

de Bruijn, D. S., ter Braak, P. M., Van de Waal, D. B., Bomer, J. G., Olthuis, W., & van den Berg, A. (2021). Calcification state of algae studied with impedance flow cytometry. In MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences: 10 - 14 October 2021, Palm Springs, CA, USA (pp. 559-560). The Chemical and Biological Microsystems Society.

de Bruijn, Douwe Sietse ; ter Braak, Paulus Martinus ; Van de Waal, Dedmer B. et al. / Calcification state of algae studied with impedance flow cytometry. MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences: 10 - 14 October 2021, Palm Springs, CA, USA. The Chemical and Biological Microsystems Society, 2021. pp. 559-560

@inproceedings{933ba3dc4178435d8b9d424cc2c48d3e,

title = "Calcification state of algae studied with impedance flow cytometry",

abstract = "The formation of calcite exoskeletons by calcifying algae plays an important role in the ocean carbon cycle. In this study we determine the average calcification state of coccolithophores based on their dielectric properties using impedance flow cytometry. This will enable us to study the effect of environmental factors on single cell calcification in a wide range of coccolithophore genotypes.",

keywords = "Calcifying algae, Impedance flow cytometry, Microfluidics, Single-cell analysis",

author = "{de Bruijn}, {Douwe Sietse} and {ter Braak}, {Paulus Martinus} and {Van de Waal}, {Dedmer B.} and Bomer, {Johan G.} and Wouter Olthuis and {van den Berg}, Albert",

note = "Funding Information: This work is part of the research program of the Foundation for Fundamental Research on Matter (FOM), which is part of the Dutch Research Council (NWO). We would also like to thank the Max Planck - Center for Complex Fluid Dynamics for support of this project and Pieter Huchshorn for his help in the cleanroom. Publisher Copyright: {\textcopyright} 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.; 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2021, MicroTAS 2021 ; Conference date: 10-10-2021 Through 14-10-2021",

year = "2021",

month = oct,

day = "11",

language = "English",

pages = "559--560",

booktitle = "MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences",

publisher = "The Chemical and Biological Microsystems Society",

url = "https://microtas2021.org/",

}

de Bruijn, DS, ter Braak, PM, Van de Waal, DB, Bomer, JG, Olthuis, W & van den Berg, A 2021, Calcification state of algae studied with impedance flow cytometry. in MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences: 10 - 14 October 2021, Palm Springs, CA, USA. The Chemical and Biological Microsystems Society, pp. 559-560, 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2021, Palm Springs, California, United States, 10/10/21.

Calcification state of algae studied with impedance flow cytometry. / de Bruijn, Douwe Sietse; ter Braak, Paulus Martinus; Van de Waal, Dedmer B. et al.
MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences: 10 - 14 October 2021, Palm Springs, CA, USA. The Chemical and Biological Microsystems Society, 2021. p. 559-560.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

TY - GEN

T1 - Calcification state of algae studied with impedance flow cytometry

AU - de Bruijn, Douwe Sietse

AU - ter Braak, Paulus Martinus

AU - Van de Waal, Dedmer B.

AU - Bomer, Johan G.

AU - Olthuis, Wouter

AU - van den Berg, Albert

N1 - Conference code: 25

PY - 2021/10/11

Y1 - 2021/10/11

N2 - The formation of calcite exoskeletons by calcifying algae plays an important role in the ocean carbon cycle. In this study we determine the average calcification state of coccolithophores based on their dielectric properties using impedance flow cytometry. This will enable us to study the effect of environmental factors on single cell calcification in a wide range of coccolithophore genotypes.

AB - The formation of calcite exoskeletons by calcifying algae plays an important role in the ocean carbon cycle. In this study we determine the average calcification state of coccolithophores based on their dielectric properties using impedance flow cytometry. This will enable us to study the effect of environmental factors on single cell calcification in a wide range of coccolithophore genotypes.

KW - Calcifying algae

KW - Impedance flow cytometry

KW - Microfluidics

KW - Single-cell analysis

M3 - Conference contribution

AN - SCOPUS:85136947948

SP - 559

EP - 560

BT - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences

PB - The Chemical and Biological Microsystems Society

T2 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2021

Y2 - 10 October 2021 through 14 October 2021

ER -

Calcification state of algae studied with impedance flow cytometry

Abstract

Conference

Keywords

Access to Document

Fingerprint

Cite this