Calcium-silicate-incorporated gellan-chitosan induced osteogenic differentiation in mesenchymal stromal cells

Krishnamurithy Genasan, Mohammad Mehrali, Tarini Veerappan, Sepehr Talebian, Murali Malliga Raman, Simmrat Singh, Sasikumar Swamiappan, Mehdi Mehrali, Tunku Kamarul*, Hanumantha Rao Balaji Raghavendran

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

11 Citations (Scopus)
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Abstract

Gellan-chitosan (GC) incorporated with CS: 0% (GC-0 CS), 10% (GC-10 CS), 20% (GC-20 CS) or 40% (GC-40 CS) w/w was prepared using freeze-drying method to investigate its physicochemical, biocompatible, and osteoinductive properties in human bone-marrow mesenchymal stromal cells (hBMSCs). The composition of different groups was reflected in physicochemical analyses performed using BET, FTIR, and XRD. The SEM micrographs revealed excellent hBMSCs attachment in GC-40 CS. The Alamar Blue assay indicated an increased proliferation and viability of seeded hBMSCs in all groups on day 21 as compared with day 0. The hBMSCs seeded in GC-40 CS indicated osteogenic differentiation based on an amplified alkaline-phosphatase release on day 7 and 14 as compared with day 0. These cells supported bone mineralization on GC-40 CS based on Alizarin-Red assay on day 21 as compared with day 7 and increased their osteogenic gene expression (RUNX2, ALP, BGLAP, BMP, and Osteonectin) on day 21. The GC-40 CS–seeded hBMSCs initiated their osteogenic differentiation on day 7 as compared with counterparts based on an increased expression of type-1 collagen and BMP2 in immunocytochemistry analysis. In conclusion, the incorporation of 40% (w/w) calcium silicate in gellan-chitosan showed osteoinduction potential in hBMSCs, making it a potential biomaterial to treat critical bone defects.

Original languageEnglish
Article number3211
Number of pages17
JournalPolymers
Volume13
Issue number19
DOIs
Publication statusPublished - 30 Sept 2021

Keywords

  • Bone
  • Bone tissue engineering
  • Calcium silicate
  • Chitosan
  • Gellan
  • Osteoinduction

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