TY - JOUR
T1 - Cancer-ID
T2 - Toward identification of cancer by tumor-derived extracellular vesicles in blood
AU - Rikkert, L.G.
AU - Beekman, P.
AU - Caro, J.
AU - Coumans, F.A.W.
AU - Enciso-Martinez, A.
AU - Jenster, G.
AU - Le Gac, S.
AU - Lee, W.
AU - van Leeuwen, T.G.
AU - Loozen, G.B.
AU - Nanou, A.
AU - Nieuwland, R.
AU - Offerhaus, H.L.
AU - Otto, C.
AU - Pegtel, D.M.
AU - Piontek, M.C.
AU - van der Pol, E.
AU - de Rond, L.
AU - Roos, W.H.
AU - Schasfoort, R.B.M.
AU - Wauben, M.H.M.
AU - Zuilhof, H.
AU - Terstappen, L.W.M.M.
PY - 2020/6/4
Y1 - 2020/6/4
N2 - Extracellular vesicles (EVs) have great potential as biomarkers since their composition and concentration in biofluids are disease state dependent and their cargo can contain disease-related information. Large tumor-derived EVs (tdEVs, >1μm) in blood from cancer patients are associated with poor outcome, and changes in their number can be used to monitor therapy effectiveness. Whereas, small tumor-derived EVs (<1μm) are likely to outnumber their larger counterparts, thereby offering better statistical significance, identification and quantification of small tdEVs are more challenging. In the blood of cancer patients, a subpopulation of EVs originate from tumor cells, but these EVs are outnumbered by non-EV particles and EVs from other origin. In the Dutch NWO Perspectief Cancer-ID program, we developed and evaluated detection and characterization techniques to distinguish EVs from non-EV particles and other EVs. Despite low signal amplitudes, we identified characteristics of these small tdEVs that may enable the enumeration of small tdEVs and extract relevant information. The insights obtained from Cancer-ID can help to explore the full potential of tdEVs in the clinic.
AB - Extracellular vesicles (EVs) have great potential as biomarkers since their composition and concentration in biofluids are disease state dependent and their cargo can contain disease-related information. Large tumor-derived EVs (tdEVs, >1μm) in blood from cancer patients are associated with poor outcome, and changes in their number can be used to monitor therapy effectiveness. Whereas, small tumor-derived EVs (<1μm) are likely to outnumber their larger counterparts, thereby offering better statistical significance, identification and quantification of small tdEVs are more challenging. In the blood of cancer patients, a subpopulation of EVs originate from tumor cells, but these EVs are outnumbered by non-EV particles and EVs from other origin. In the Dutch NWO Perspectief Cancer-ID program, we developed and evaluated detection and characterization techniques to distinguish EVs from non-EV particles and other EVs. Despite low signal amplitudes, we identified characteristics of these small tdEVs that may enable the enumeration of small tdEVs and extract relevant information. The insights obtained from Cancer-ID can help to explore the full potential of tdEVs in the clinic.
UR - http://www.scopus.com/inward/record.url?scp=85087360476&partnerID=8YFLogxK
U2 - 10.3389/fonc.2020.00608
DO - 10.3389/fonc.2020.00608
M3 - Article
AN - SCOPUS:85087360476
SN - 2234-943X
VL - 10
JO - Frontiers in oncology
JF - Frontiers in oncology
M1 - 608
ER -
