Carotenoid levels in lymphocytes obtained from peripheral blood of healthy people have been investigated by Raman microspectroscopy. We observed that carotenoids are concentrated in so-called “Gall bodies”. The level of carotenoids in living human lymphocytes was found to be age-dependent and to decrease with age. We performed carotenoid uptake experiments using liposomes containing beta-carotene (egg phosphatidylcholine and egg phosphatidylglycerol, 10/1 molar ratio). We observed that beta-carotene is rapidly taken up in vitro, and transported to the Gall body. From these findings we conclude that Raman microspectroscopy is a sensitive method to determine carotenoid levels in single living cells.