Carotenoid levels in human lymphocytes, measured by Raman microspectroscopy

R. Ramanauskaite, Regina B. Ramanauskaite, Gezina M.J. Segers-Nolten, C.J. de Grauw, Kees J. de Grauw, N.M. Sijtsema, Louis van der Maas, Jan Greve, Cornelis Otto, Carl Figdor

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Carotenoid levels in lymphocytes obtained from peripheral blood of healthy people have been investigated by Raman microspectroscopy. We observed that carotenoids are concentrated in so-called “Gall bodies”. The level of carotenoids in living human lymphocytes was found to be age-dependent and to decrease with age. We performed carotenoid uptake experiments using liposomes containing beta-carotene (egg phosphatidylcholine and egg phosphatidylglycerol, 10/1 molar ratio). We observed that beta-carotene is rapidly taken up in vitro, and transported to the Gall body. From these findings we conclude that Raman microspectroscopy is a sensitive method to determine carotenoid levels in single living cells.
Original languageUndefined
Pages (from-to)2131-2134
Number of pages4
JournalPure and applied chemistry
Issue number10
Publication statusPublished - 1997


  • METIS-129314
  • IR-96418

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