Carotenoids located in human lymphocyte subpopulations and Natural Killer cells by Raman microspectroscopy

G.J. Puppels, H.S.P. Garritsen, J.A. Kummer, Jan Greve

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    Abstract

    The presence and subcellular location of carotenoids in human lymphocyte sub-populations (CD4+, CD8+, T-cell receptor-γδ+, and CD19+ ) and natural killer cells (CD16+ ) were studied by means of Raman microspectroscopy. In CD4+ lymphocytes a high concentration (10-3M) of carotenoids was found in the Gall body. In CD8+ lymphocytes, T-cell-receptor-γδ+ lymphocytes and in natural killer cells carotenoids appeared to be concentrated (10-4M) in the Golgi complex. The concentration of carotenoids in CD19+ lymphocytes was found to be below the present detection limit, which is ~10-6 to 10-5 M. The results provide new possibilities to investigate the mechanism(s) behind the suggested protective role of carotenoids against the development of cancers.
    Original languageEnglish
    Pages (from-to)251-256
    Number of pages7
    JournalCytometry
    Volume14
    Issue number3
    DOIs
    Publication statusPublished - 1993

    Keywords

    • Cancer
    • Fluorescence activated cell sorting
    • Golgi complex
    • METIS-129491
    • fluorescence microscopy
    • IR-60728
    • Gall body

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