Carotenoids located in human lymphocyte subpopulations and Natural Killer cells by Raman microspectroscopy

G.J. Puppels; H.S.P. Garritsen; J.A. Kummer; Jan Greve

doi:10.1002/cyto.990140303

Carotenoids located in human lymphocyte subpopulations and Natural Killer cells by Raman microspectroscopy

G.J. Puppels, H.S.P. Garritsen, J.A. Kummer, Jan Greve

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Abstract

The presence and subcellular location of carotenoids in human lymphocyte sub-populations (CD4+, CD8+, T-cell receptor-γδ+, and CD19+ ) and natural killer cells (CD16+ ) were studied by means of Raman microspectroscopy. In CD4+ lymphocytes a high concentration (10-3M) of carotenoids was found in the Gall body. In CD8+ lymphocytes, T-cell-receptor-γδ+ lymphocytes and in natural killer cells carotenoids appeared to be concentrated (10-4M) in the Golgi complex. The concentration of carotenoids in CD19+ lymphocytes was found to be below the present detection limit, which is ~10-6 to 10-5 M. The results provide new possibilities to investigate the mechanism(s) behind the suggested protective role of carotenoids against the development of cancers.

Original language	English
Pages (from-to)	251-256
Number of pages	7
Journal	Cytometry
Volume	14
Issue number	3
DOIs	https://doi.org/10.1002/cyto.990140303
Publication status	Published - 1993

Keywords

Cancer
Fluorescence activated cell sorting
Golgi complex
METIS-129491
fluorescence microscopy
IR-60728
Gall body

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title = "Carotenoids located in human lymphocyte subpopulations and Natural Killer cells by Raman microspectroscopy",

abstract = "The presence and subcellular location of carotenoids in human lymphocyte sub-populations (CD4+, CD8+, T-cell receptor-γδ+, and CD19+ ) and natural killer cells (CD16+ ) were studied by means of Raman microspectroscopy. In CD4+ lymphocytes a high concentration (10-3M) of carotenoids was found in the Gall body. In CD8+ lymphocytes, T-cell-receptor-γδ+ lymphocytes and in natural killer cells carotenoids appeared to be concentrated (10-4M) in the Golgi complex. The concentration of carotenoids in CD19+ lymphocytes was found to be below the present detection limit, which is ~10-6 to 10-5 M. The results provide new possibilities to investigate the mechanism(s) behind the suggested protective role of carotenoids against the development of cancers.",

keywords = "Cancer, Fluorescence activated cell sorting, Golgi complex, METIS-129491, fluorescence microscopy, IR-60728, Gall body",

author = "G.J. Puppels and H.S.P. Garritsen and J.A. Kummer and Jan Greve",

year = "1993",

doi = "10.1002/cyto.990140303",

language = "English",

volume = "14",

pages = "251--256",

journal = "Cytometry",

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publisher = "Wiley",

number = "3",

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TY - JOUR

T1 - Carotenoids located in human lymphocyte subpopulations and Natural Killer cells by Raman microspectroscopy

AU - Puppels, G.J.

AU - Garritsen, H.S.P.

AU - Kummer, J.A.

AU - Greve, Jan

PY - 1993

Y1 - 1993

N2 - The presence and subcellular location of carotenoids in human lymphocyte sub-populations (CD4+, CD8+, T-cell receptor-γδ+, and CD19+ ) and natural killer cells (CD16+ ) were studied by means of Raman microspectroscopy. In CD4+ lymphocytes a high concentration (10-3M) of carotenoids was found in the Gall body. In CD8+ lymphocytes, T-cell-receptor-γδ+ lymphocytes and in natural killer cells carotenoids appeared to be concentrated (10-4M) in the Golgi complex. The concentration of carotenoids in CD19+ lymphocytes was found to be below the present detection limit, which is ~10-6 to 10-5 M. The results provide new possibilities to investigate the mechanism(s) behind the suggested protective role of carotenoids against the development of cancers.

AB - The presence and subcellular location of carotenoids in human lymphocyte sub-populations (CD4+, CD8+, T-cell receptor-γδ+, and CD19+ ) and natural killer cells (CD16+ ) were studied by means of Raman microspectroscopy. In CD4+ lymphocytes a high concentration (10-3M) of carotenoids was found in the Gall body. In CD8+ lymphocytes, T-cell-receptor-γδ+ lymphocytes and in natural killer cells carotenoids appeared to be concentrated (10-4M) in the Golgi complex. The concentration of carotenoids in CD19+ lymphocytes was found to be below the present detection limit, which is ~10-6 to 10-5 M. The results provide new possibilities to investigate the mechanism(s) behind the suggested protective role of carotenoids against the development of cancers.

KW - Cancer

KW - Fluorescence activated cell sorting

KW - Golgi complex

KW - METIS-129491

KW - fluorescence microscopy

KW - IR-60728

KW - Gall body

U2 - 10.1002/cyto.990140303

DO - 10.1002/cyto.990140303

M3 - Article

VL - 14

SP - 251

EP - 256

JO - Cytometry

JF - Cytometry

SN - 0196-4763

IS - 3

ER -

Carotenoids located in human lymphocyte subpopulations and Natural Killer cells by Raman microspectroscopy

Abstract

Keywords

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