The presence and subcellular location of carotenoids in human lymphocyte sub-populations (CD4+, CD8+, T-cell receptor-γδ+, and CD19+ ) and natural killer cells (CD16+ ) were studied by means of Raman microspectroscopy. In CD4+ lymphocytes a high concentration (10-3M) of carotenoids was found in the Gall body. In CD8+ lymphocytes, T-cell-receptor-γδ+ lymphocytes and in natural killer cells carotenoids appeared to be concentrated (10-4M) in the Golgi complex. The concentration of carotenoids in CD19+ lymphocytes was found to be below the present detection limit, which is ~10-6 to 10-5 M. The results provide new possibilities to investigate the mechanism(s) behind the suggested protective role of carotenoids against the development of cancers.
- Fluorescence activated cell sorting
- Golgi complex
- fluorescence microscopy
- Gall body