CARS and Raman spectroscopy of function-related conformational changes of chymotrypsin

N.N. Brandt; A.Yu. Chikishev; A.Y. Chikishev; Jan Greve; N.I. Koroteev; Cornelis Otto; I.K. Sakodinskaya; I.K. Sakodynskaya

doi:10.1002/1097-4555(200008/09)31:8/9<731::AID-JRS609>3.0.CO;2-0

CARS and Raman spectroscopy of function-related conformational changes of chymotrypsin

N.N. Brandt, A.Yu. Chikishev, A.Y. Chikishev, Jan Greve, N.I. Koroteev, Cornelis Otto, I.K. Sakodinskaya, I.K. Sakodynskaya

Research output: Contribution to journal › Article › Academic › peer-review

11 Citations (Scopus)

Abstract

We report on the comparative analysis of the conformation-sensitive bands of free enzyme (chymotrypsin), liganded enzyme (chymotrypsin anthranilate) and enzyme complex with 18-crown-6. The studies were carried out by Raman scattering spectroscopy and polarization-sensitive coherent anti-Stokes Raman scattering spectroscopy (CARS). The bands of tyrosine and tryptophan residues, disulfide bridges and amide I and amide III were analyzed. It is concluded that the conformation of the enzyme changes much more in the case of liganding than in the case of the formation of a complex with the crown ether.

Original language	Undefined
Pages (from-to)	731-737
Number of pages	7
Journal	Journal of raman spectroscopy
Volume	31
Issue number	8-9
DOIs	https://doi.org/10.1002/1097-4555(200008/09)31:8/9<731::AID-JRS609>3.0.CO;2-0
Publication status	Published - 2000

Keywords

IR-71574
METIS-129343

Access to Document

10.1002/1097-4555(200008/09)31:8/9<731::AID-JRS609>3.0.CO;2-0

Cite this

@article{297d198437dd4ce9982880c35fa49a6b,

title = "CARS and Raman spectroscopy of function-related conformational changes of chymotrypsin",

abstract = "We report on the comparative analysis of the conformation-sensitive bands of free enzyme (chymotrypsin), liganded enzyme (chymotrypsin anthranilate) and enzyme complex with 18-crown-6. The studies were carried out by Raman scattering spectroscopy and polarization-sensitive coherent anti-Stokes Raman scattering spectroscopy (CARS). The bands of tyrosine and tryptophan residues, disulfide bridges and amide I and amide III were analyzed. It is concluded that the conformation of the enzyme changes much more in the case of liganding than in the case of the formation of a complex with the crown ether.",

keywords = "IR-71574, METIS-129343",

author = "N.N. Brandt and A.Yu. Chikishev and A.Y. Chikishev and Jan Greve and N.I. Koroteev and Cornelis Otto and I.K. Sakodinskaya and I.K. Sakodynskaya",

year = "2000",

doi = "10.1002/1097-4555(200008/09)31:8/9<731::AID-JRS609>3.0.CO;2-0",

language = "Undefined",

volume = "31",

pages = "731--737",

journal = "Journal of raman spectroscopy",

issn = "0377-0486",

publisher = "Wiley",

number = "8-9",

}

CARS and Raman spectroscopy of function-related conformational changes of chymotrypsin. / Brandt, N.N.; Chikishev, A.Yu.; Chikishev, A.Y.; Greve, Jan; Koroteev, N.I.; Otto, Cornelis; Sakodinskaya, I.K.; Sakodynskaya, I.K.

In: Journal of raman spectroscopy, Vol. 31, No. 8-9, 2000, p. 731-737.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - CARS and Raman spectroscopy of function-related conformational changes of chymotrypsin

AU - Brandt, N.N.

AU - Chikishev, A.Yu.

AU - Chikishev, A.Y.

AU - Greve, Jan

AU - Koroteev, N.I.

AU - Otto, Cornelis

AU - Sakodinskaya, I.K.

AU - Sakodynskaya, I.K.

PY - 2000

Y1 - 2000

N2 - We report on the comparative analysis of the conformation-sensitive bands of free enzyme (chymotrypsin), liganded enzyme (chymotrypsin anthranilate) and enzyme complex with 18-crown-6. The studies were carried out by Raman scattering spectroscopy and polarization-sensitive coherent anti-Stokes Raman scattering spectroscopy (CARS). The bands of tyrosine and tryptophan residues, disulfide bridges and amide I and amide III were analyzed. It is concluded that the conformation of the enzyme changes much more in the case of liganding than in the case of the formation of a complex with the crown ether.

AB - We report on the comparative analysis of the conformation-sensitive bands of free enzyme (chymotrypsin), liganded enzyme (chymotrypsin anthranilate) and enzyme complex with 18-crown-6. The studies were carried out by Raman scattering spectroscopy and polarization-sensitive coherent anti-Stokes Raman scattering spectroscopy (CARS). The bands of tyrosine and tryptophan residues, disulfide bridges and amide I and amide III were analyzed. It is concluded that the conformation of the enzyme changes much more in the case of liganding than in the case of the formation of a complex with the crown ether.

KW - IR-71574

KW - METIS-129343

U2 - 10.1002/1097-4555(200008/09)31:8/9<731::AID-JRS609>3.0.CO;2-0

DO - 10.1002/1097-4555(200008/09)31:8/9<731::AID-JRS609>3.0.CO;2-0

M3 - Article

VL - 31

SP - 731

EP - 737

JO - Journal of raman spectroscopy

JF - Journal of raman spectroscopy

SN - 0377-0486

IS - 8-9

ER -