CARS and Raman spectroscopy of function-related conformational changes of chymotrypsin

N.N. Brandt, A.Yu. Chikishev, A.Y. Chikishev, Jan Greve, N.I. Koroteev, Cornelis Otto, I.K. Sakodinskaya, I.K. Sakodynskaya

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11 Citations (Scopus)

Abstract

We report on the comparative analysis of the conformation-sensitive bands of free enzyme (chymotrypsin), liganded enzyme (chymotrypsin anthranilate) and enzyme complex with 18-crown-6. The studies were carried out by Raman scattering spectroscopy and polarization-sensitive coherent anti-Stokes Raman scattering spectroscopy (CARS). The bands of tyrosine and tryptophan residues, disulfide bridges and amide I and amide III were analyzed. It is concluded that the conformation of the enzyme changes much more in the case of liganding than in the case of the formation of a complex with the crown ether.
Original languageUndefined
Pages (from-to)731-737
Number of pages7
JournalJournal of raman spectroscopy
Volume31
Issue number8-9
DOIs
Publication statusPublished - 2000

Keywords

  • IR-71574
  • METIS-129343

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