Abstract
Protein-based nanoreactors are generated by encapsulating an enzyme inside the capsid of the cowpea chlorotic mottle virus (CCMV). Here, three different noncovalent methods are described to efficiently incorporate enzymes inside the capsid of these viral protein cages. The methods are based on pH, leucine zippers, and electrostatic interactions respectively, as a driving force for encapsulation. The methods are exclusively described for the enzymes horseradish peroxidase, glucose oxidase, and Pseudozyma antarctica lipase B, but they are also applicable for other enzymes.
| Original language | English |
|---|---|
| Title of host publication | Methods in Molecular Biology |
| Publisher | Humana Press Inc. |
| Pages | 237-247 |
| Number of pages | 11 |
| Volume | 1776 |
| DOIs | |
| Publication status | Published - 1 Jan 2018 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 1776 |
| ISSN (Print) | 1064-3745 |
Fingerprint
Keywords
- Cowpea chlorotic mottle virus (CCMV)
- Enzyme encapsulation
- Functional cargo
- Leucine zippers
- Nanoreactors
- pH-responsive assembly
- Virus-like particles
Cite this
}
CCMV-based enzymatic nanoreactors. / de Ruiter, Mark V.; Putri, Rindia M.; Cornelissen, Jeroen J.L.M.
Methods in Molecular Biology. Vol. 1776 Humana Press Inc., 2018. p. 237-247 (Methods in Molecular Biology; Vol. 1776).Research output: Chapter in Book/Report/Conference proceeding › Chapter › Academic › peer-review
TY - CHAP
T1 - CCMV-based enzymatic nanoreactors
AU - de Ruiter, Mark V.
AU - Putri, Rindia M.
AU - Cornelissen, Jeroen J.L.M.
PY - 2018/1/1
Y1 - 2018/1/1
N2 - Protein-based nanoreactors are generated by encapsulating an enzyme inside the capsid of the cowpea chlorotic mottle virus (CCMV). Here, three different noncovalent methods are described to efficiently incorporate enzymes inside the capsid of these viral protein cages. The methods are based on pH, leucine zippers, and electrostatic interactions respectively, as a driving force for encapsulation. The methods are exclusively described for the enzymes horseradish peroxidase, glucose oxidase, and Pseudozyma antarctica lipase B, but they are also applicable for other enzymes.
AB - Protein-based nanoreactors are generated by encapsulating an enzyme inside the capsid of the cowpea chlorotic mottle virus (CCMV). Here, three different noncovalent methods are described to efficiently incorporate enzymes inside the capsid of these viral protein cages. The methods are based on pH, leucine zippers, and electrostatic interactions respectively, as a driving force for encapsulation. The methods are exclusively described for the enzymes horseradish peroxidase, glucose oxidase, and Pseudozyma antarctica lipase B, but they are also applicable for other enzymes.
KW - Cowpea chlorotic mottle virus (CCMV)
KW - Enzyme encapsulation
KW - Functional cargo
KW - Leucine zippers
KW - Nanoreactors
KW - pH-responsive assembly
KW - Virus-like particles
UR - http://www.scopus.com/inward/record.url?scp=85048209964&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-7808-3_16
DO - 10.1007/978-1-4939-7808-3_16
M3 - Chapter
VL - 1776
T3 - Methods in Molecular Biology
SP - 237
EP - 247
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -