CCMV-based enzymatic nanoreactors

Mark V. de Ruiter, Rindia M. Putri, Jeroen J.L.M. Cornelissen*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

4 Citations (Scopus)

Abstract

Protein-based nanoreactors are generated by encapsulating an enzyme inside the capsid of the cowpea chlorotic mottle virus (CCMV). Here, three different noncovalent methods are described to efficiently incorporate enzymes inside the capsid of these viral protein cages. The methods are based on pH, leucine zippers, and electrostatic interactions respectively, as a driving force for encapsulation. The methods are exclusively described for the enzymes horseradish peroxidase, glucose oxidase, and Pseudozyma antarctica lipase B, but they are also applicable for other enzymes.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages237-247
Number of pages11
Volume1776
DOIs
Publication statusPublished - 1 Jan 2018

Publication series

NameMethods in Molecular Biology
Volume1776
ISSN (Print)1064-3745

Keywords

  • Cowpea chlorotic mottle virus (CCMV)
  • Enzyme encapsulation
  • Functional cargo
  • Leucine zippers
  • Nanoreactors
  • pH-responsive assembly
  • Virus-like particles

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    de Ruiter, M. V., Putri, R. M., & Cornelissen, J. J. L. M. (2018). CCMV-based enzymatic nanoreactors. In Methods in Molecular Biology (Vol. 1776, pp. 237-247). (Methods in Molecular Biology; Vol. 1776). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7808-3_16