Abstract
Protein-based nanoreactors are generated by encapsulating an enzyme inside the capsid of the cowpea chlorotic mottle virus (CCMV). Here, three different noncovalent methods are described to efficiently incorporate enzymes inside the capsid of these viral protein cages. The methods are based on pH, leucine zippers, and electrostatic interactions respectively, as a driving force for encapsulation. The methods are exclusively described for the enzymes horseradish peroxidase, glucose oxidase, and Pseudozyma antarctica lipase B, but they are also applicable for other enzymes.
| Original language | English |
|---|---|
| Title of host publication | Methods in Molecular Biology |
| Publisher | Humana Press Inc. |
| Pages | 237-247 |
| Number of pages | 11 |
| Volume | 1776 |
| DOIs | |
| Publication status | Published - 1 Jan 2018 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 1776 |
| ISSN (Print) | 1064-3745 |
Keywords
- Cowpea chlorotic mottle virus (CCMV)
- Enzyme encapsulation
- Functional cargo
- Leucine zippers
- Nanoreactors
- pH-responsive assembly
- Virus-like particles
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Cite this
de Ruiter, M. V., Putri, R. M., & Cornelissen, J. J. L. M. (2018). CCMV-based enzymatic nanoreactors. In Methods in Molecular Biology (Vol. 1776, pp. 237-247). (Methods in Molecular Biology; Vol. 1776). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7808-3_16