TY - CHAP
T1 - CCMV-based enzymatic nanoreactors
AU - de Ruiter, Mark V.
AU - Putri, Rindia M.
AU - Cornelissen, Jeroen J.L.M.
PY - 2018/1/1
Y1 - 2018/1/1
N2 - Protein-based nanoreactors are generated by encapsulating an enzyme inside the capsid of the cowpea chlorotic mottle virus (CCMV). Here, three different noncovalent methods are described to efficiently incorporate enzymes inside the capsid of these viral protein cages. The methods are based on pH, leucine zippers, and electrostatic interactions respectively, as a driving force for encapsulation. The methods are exclusively described for the enzymes horseradish peroxidase, glucose oxidase, and Pseudozyma antarctica lipase B, but they are also applicable for other enzymes.
AB - Protein-based nanoreactors are generated by encapsulating an enzyme inside the capsid of the cowpea chlorotic mottle virus (CCMV). Here, three different noncovalent methods are described to efficiently incorporate enzymes inside the capsid of these viral protein cages. The methods are based on pH, leucine zippers, and electrostatic interactions respectively, as a driving force for encapsulation. The methods are exclusively described for the enzymes horseradish peroxidase, glucose oxidase, and Pseudozyma antarctica lipase B, but they are also applicable for other enzymes.
KW - Cowpea chlorotic mottle virus (CCMV)
KW - Enzyme encapsulation
KW - Functional cargo
KW - Leucine zippers
KW - Nanoreactors
KW - pH-responsive assembly
KW - Virus-like particles
UR - http://www.scopus.com/inward/record.url?scp=85048209964&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-7808-3_16
DO - 10.1007/978-1-4939-7808-3_16
M3 - Chapter
AN - SCOPUS:85048209964
VL - 1776
T3 - Methods in Molecular Biology
SP - 237
EP - 247
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -