Cell Capture and Lysis on a Chip

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    Abstract

    Single cell analysis in a microfluidic device proceeds through a certain number of steps. The two first steps of the protocol are the manipulation of individual cells in the microfluidic device and their positioning in precise locations and subsequently the process to rupture the cell membrane to retrieve the cellular content. In this chapter, we will focus on these two first steps of cell trapping and single cell lysis, and review different approaches and techniques found in the literature for these two steps to achieve these in a microfluidic format. We will also discuss the advantages and limitations of the different techniques of manipulation, trapping and lysis.
    Original languageUndefined
    Title of host publicationUnravelling Single Cell Genomics: Micro and Nanotools
    EditorsNathalie Bontoux, Luce Dauphinot, Marie-Claude Potier
    Place of PublicationCambridge
    PublisherRoyal Society of Chemistry
    Pages150-184
    Number of pages35
    ISBN (Print)978-1-84755-911-1
    DOIs
    Publication statusPublished - 2010

    Publication series

    NameRSC Nanoscience & Nanotechnology
    PublisherRoyal Society of Chemistry
    Number15
    Volume15
    ISSN (Print)1757-7136

    Keywords

    • METIS-276317
    • EWI-19432
    • IR-75854

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