Cell detachment method using shock wave induced cavitation

L. Junge, L. Junge, C.D. Ohl, B. Wolfrum, M. Arora, R. Ikink

Research output: Contribution to journalArticleAcademicpeer-review

24 Citations (Scopus)

Abstract

The detachment of adherent HeLa cells from a substrate after the interaction with a shock wave is analyzed. Cavitation bubbles are formed in the trailing, negative pressure cycle following the shock front. We find that the regions of cell detachment are strongly correlated with spatial presence of cavitation bubbles. It is shown that the cavitation bubble collapse generates a transient high-speed flow along the substrate surface leading to rapid detachment of the cells. Flow trajectories are reconstructed from the video recordings using robust image-processing methods. From these trajectories, an estimate of the shear stress acting on the cells is obtained and the area of detachment is estimated with a kinetic model. Furthermore, it is suggested that the application of shock waves extends the known methods of cell detachment with the ability to control the process in space and time.
Original languageUndefined
Pages (from-to)1769-1776
Number of pages8
JournalUltrasound in medicine and biology
Volume29
Issue number12
DOIs
Publication statusPublished - 2003

Keywords

  • Particle tracking velocimetry
  • Shock wave
  • Adherent cells
  • Cavitation
  • Cell detachment
  • IR-74958
  • METIS-216924

Cite this

Junge, L., Junge, L., Ohl, C. D., Wolfrum, B., Arora, M., & Ikink, R. (2003). Cell detachment method using shock wave induced cavitation. Ultrasound in medicine and biology, 29(12), 1769-1776. https://doi.org/10.1016/j.ultrasmedbio.2003.08.010
Junge, L. ; Junge, L. ; Ohl, C.D. ; Wolfrum, B. ; Arora, M. ; Ikink, R. / Cell detachment method using shock wave induced cavitation. In: Ultrasound in medicine and biology. 2003 ; Vol. 29, No. 12. pp. 1769-1776.
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Junge, L, Junge, L, Ohl, CD, Wolfrum, B, Arora, M & Ikink, R 2003, 'Cell detachment method using shock wave induced cavitation', Ultrasound in medicine and biology, vol. 29, no. 12, pp. 1769-1776. https://doi.org/10.1016/j.ultrasmedbio.2003.08.010

Cell detachment method using shock wave induced cavitation. / Junge, L.; Junge, L.; Ohl, C.D.; Wolfrum, B.; Arora, M.; Ikink, R.

In: Ultrasound in medicine and biology, Vol. 29, No. 12, 2003, p. 1769-1776.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Cell detachment method using shock wave induced cavitation

AU - Junge, L.

AU - Junge, L.

AU - Ohl, C.D.

AU - Wolfrum, B.

AU - Arora, M.

AU - Ikink, R.

PY - 2003

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AB - The detachment of adherent HeLa cells from a substrate after the interaction with a shock wave is analyzed. Cavitation bubbles are formed in the trailing, negative pressure cycle following the shock front. We find that the regions of cell detachment are strongly correlated with spatial presence of cavitation bubbles. It is shown that the cavitation bubble collapse generates a transient high-speed flow along the substrate surface leading to rapid detachment of the cells. Flow trajectories are reconstructed from the video recordings using robust image-processing methods. From these trajectories, an estimate of the shear stress acting on the cells is obtained and the area of detachment is estimated with a kinetic model. Furthermore, it is suggested that the application of shock waves extends the known methods of cell detachment with the ability to control the process in space and time.

KW - Particle tracking velocimetry

KW - Shock wave

KW - Adherent cells

KW - Cavitation

KW - Cell detachment

KW - IR-74958

KW - METIS-216924

U2 - 10.1016/j.ultrasmedbio.2003.08.010

DO - 10.1016/j.ultrasmedbio.2003.08.010

M3 - Article

VL - 29

SP - 1769

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JO - Ultrasound in medicine and biology

JF - Ultrasound in medicine and biology

SN - 0301-5629

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ER -