Cell permeabilisation and transport focused around oscillating microbubbles

P.G.M. Marmottant; Sascha Hilgenfeldt; Michel Versluis; Johannes G.E. Gardeniers; Albert van den Berg; Detlef Lohse

Cell permeabilisation and transport focused around oscillating microbubbles

P.G.M. Marmottant, Sascha Hilgenfeldt, Michel Versluis, Johannes G.E. Gardeniers, Albert van den Berg, Detlef Lohse

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

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Abstract

The ultrasound-driven oscillation of a microbubble drives a steady streaming focused around the bubble. The study of individual bubbles attached to a wall shows vivid recirculations. When cells are in the vicinity of these bubbles, also used in medecine as contrast agent for ultrasound echography, they experience considerable shear rates. We introduce in the flow giant unilamelar lipid vesicles, acting as artificial cells. Rupture of the lipidic membrane with the opening of pores is revealed by high-speed camera recordings. A reversible permeation of the membrane wall can also be obtained, demonstrating at the micron scale the efficiency of microbubbles to deliver drugs in cells. The streaming flow of bubble on a surface can be further controlled, with the adjunction of a solid obstacle nearby: the flow turns to be directed. We will present a microfluidic device using the principle of bubble/obstacle doublets to locally transport small objets such as cells.

Original language	Undefined
Title of host publication	Mechanics of the 21st century: 21st International Congress of Theoretical and Applied Mechanics
Editors	Witold Gutkowski, Tomasz A. Kowalewski
Place of Publication	Warsaw, Poland
Publisher	Springer
Pages	-
ISBN (Print)	9781402035593
Publication status	Published - 15 Aug 2004
Event	XXI International Congress of Theoretical and Applied Mechanics, ICTAM 2004 - Warsaw, Poland Duration: 15 Aug 2004 → 21 Aug 2004 Conference number: 21

Publication series

Name
Publisher	Springer

Conference

Conference	XXI International Congress of Theoretical and Applied Mechanics, ICTAM 2004
Abbreviated title	ICTAM
Country/Territory	Poland
City	Warsaw
Period	15/08/04 → 21/08/04

Keywords

METIS-218778
IR-47844

Access to Document

Marmottant04cell

http://extras.springer.com/2005/978-94-007-8907-4/ebook

Cite this

Marmottant, P. G. M., Hilgenfeldt, S., Versluis, M., Gardeniers, J. G. E., van den Berg, A., & Lohse, D. (2004). Cell permeabilisation and transport focused around oscillating microbubbles. In W. Gutkowski, & T. A. Kowalewski (Eds.), Mechanics of the 21st century: 21st International Congress of Theoretical and Applied Mechanics (pp. -). Springer. http://extras.springer.com/2005/978-94-007-8907-4/ebook

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title = "Cell permeabilisation and transport focused around oscillating microbubbles",

abstract = "The ultrasound-driven oscillation of a microbubble drives a steady streaming focused around the bubble. The study of individual bubbles attached to a wall shows vivid recirculations. When cells are in the vicinity of these bubbles, also used in medecine as contrast agent for ultrasound echography, they experience considerable shear rates. We introduce in the flow giant unilamelar lipid vesicles, acting as artificial cells. Rupture of the lipidic membrane with the opening of pores is revealed by high-speed camera recordings. A reversible permeation of the membrane wall can also be obtained, demonstrating at the micron scale the efficiency of microbubbles to deliver drugs in cells. The streaming flow of bubble on a surface can be further controlled, with the adjunction of a solid obstacle nearby: the flow turns to be directed. We will present a microfluidic device using the principle of bubble/obstacle doublets to locally transport small objets such as cells.",

keywords = "METIS-218778, IR-47844",

author = "P.G.M. Marmottant and Sascha Hilgenfeldt and Michel Versluis and Gardeniers, {Johannes G.E.} and {van den Berg}, Albert and Detlef Lohse",

year = "2004",

month = aug,

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isbn = "9781402035593",

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editor = "Witold Gutkowski and Kowalewski, {Tomasz A.}",

booktitle = "Mechanics of the 21st century: 21st International Congress of Theoretical and Applied Mechanics",

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Marmottant, PGM, Hilgenfeldt, S, Versluis, M , Gardeniers, JGE , van den Berg, A & Lohse, D 2004, Cell permeabilisation and transport focused around oscillating microbubbles. in W Gutkowski & TA Kowalewski (eds), Mechanics of the 21st century: 21st International Congress of Theoretical and Applied Mechanics. Springer, Warsaw, Poland, pp. -, XXI International Congress of Theoretical and Applied Mechanics, ICTAM 2004, Warsaw, Poland, 15/08/04. <http://extras.springer.com/2005/978-94-007-8907-4/ebook>

Cell permeabilisation and transport focused around oscillating microbubbles. / Marmottant, P.G.M.; Hilgenfeldt, Sascha; Versluis, Michel et al.
Mechanics of the 21st century: 21st International Congress of Theoretical and Applied Mechanics. ed. / Witold Gutkowski; Tomasz A. Kowalewski. Warsaw, Poland: Springer, 2004. p. -.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

TY - GEN

T1 - Cell permeabilisation and transport focused around oscillating microbubbles

AU - Marmottant, P.G.M.

AU - Hilgenfeldt, Sascha

AU - Versluis, Michel

AU - Gardeniers, Johannes G.E.

AU - van den Berg, Albert

AU - Lohse, Detlef

N1 - Conference code: 21

PY - 2004/8/15

Y1 - 2004/8/15

N2 - The ultrasound-driven oscillation of a microbubble drives a steady streaming focused around the bubble. The study of individual bubbles attached to a wall shows vivid recirculations. When cells are in the vicinity of these bubbles, also used in medecine as contrast agent for ultrasound echography, they experience considerable shear rates. We introduce in the flow giant unilamelar lipid vesicles, acting as artificial cells. Rupture of the lipidic membrane with the opening of pores is revealed by high-speed camera recordings. A reversible permeation of the membrane wall can also be obtained, demonstrating at the micron scale the efficiency of microbubbles to deliver drugs in cells. The streaming flow of bubble on a surface can be further controlled, with the adjunction of a solid obstacle nearby: the flow turns to be directed. We will present a microfluidic device using the principle of bubble/obstacle doublets to locally transport small objets such as cells.

AB - The ultrasound-driven oscillation of a microbubble drives a steady streaming focused around the bubble. The study of individual bubbles attached to a wall shows vivid recirculations. When cells are in the vicinity of these bubbles, also used in medecine as contrast agent for ultrasound echography, they experience considerable shear rates. We introduce in the flow giant unilamelar lipid vesicles, acting as artificial cells. Rupture of the lipidic membrane with the opening of pores is revealed by high-speed camera recordings. A reversible permeation of the membrane wall can also be obtained, demonstrating at the micron scale the efficiency of microbubbles to deliver drugs in cells. The streaming flow of bubble on a surface can be further controlled, with the adjunction of a solid obstacle nearby: the flow turns to be directed. We will present a microfluidic device using the principle of bubble/obstacle doublets to locally transport small objets such as cells.

KW - METIS-218778

KW - IR-47844

M3 - Conference contribution

SN - 9781402035593

SP - -

BT - Mechanics of the 21st century: 21st International Congress of Theoretical and Applied Mechanics

A2 - Gutkowski, Witold

A2 - Kowalewski, Tomasz A.

PB - Springer

CY - Warsaw, Poland

T2 - XXI International Congress of Theoretical and Applied Mechanics, ICTAM 2004

Y2 - 15 August 2004 through 21 August 2004

ER -