CellTracks cytometer for detection of circulating tumor cells

A.G.J. Tibbe, A. van der Kooi, M.R. de Groot, I. Vermes

Research output: Contribution to conferencePosterOther research output

Abstract

Introduction: In patients with carcinomas, tumor cells are shed into the circulation. The number of the circulating tumor cells is low and technology is needed that has sufficient sensitivity and specificity to enumerate and characterize these cells. The CellTracks system was developed to provide an immunophenotype, fluorescence wave forms as well as images of immuno-magnetically enriched tumor cells with an occurrence frequency <1 per ml whole blood. Methods: 7.5 ml of whole blood of patients having known breast (n=13) or colorectal (n=12) cancers was immunomagnetically enriched for EpCam positive (epithelial) cells reducing the volume to 320 μl. Fluorescent labels against Cytokeratin and DNA were added. As a negative control a CD45 labelled fluorescent probe against white blood cells was used. This sample was transferred to a measuring chamber, which was inserted in a specially designed strong magnetic field. The magnetic force aligns the cells on a glass slide. These cells were scanned one by one by a multi-laser scanning system and their fluorescence intensities were measured. As a control samples of healthy volunteers were measured in duplicate. To test the immuno-magnetic collection efficiency a known number of fixed SKBR-3 cells were spiked into blood samples. Results: In all patients samples tumor cells were detected. The number of tumor cells found ranged from 1-120 per 7.5 ml of whole blood. For the control samples 0-1 positive cells per 7.5 ml have been detected. The overall immuno-magnetic selection and detection efficiency for SKBR-3 cells was > 70 %. Conclusions: We showed that the novel CellTracks technology has a very high sensitivity and specificity, which is sufficient for the detection of tumor, cells in peripheral blood.
Original languageEnglish
Publication statusPublished - 16 Apr 2002
Event56e Voorjaarscongres NVKC 2003 - Lunteren, Netherlands
Duration: 16 Apr 200317 Apr 2003

Conference

Conference56e Voorjaarscongres NVKC 2003
CountryNetherlands
CityLunteren
Period16/04/0317/04/03

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Circulating Neoplastic Cells
Neoplasms
Fluorescence
Technology
Sensitivity and Specificity
Magnetic Fields
Keratins
Fluorescent Dyes
Cell Size
Glass
Healthy Volunteers
Breast
Lasers
Leukocytes
Cell Count
Epithelial Cells
Carcinoma

Cite this

Tibbe, A. G. J., van der Kooi, A., de Groot, M. R., & Vermes, I. (2002). CellTracks cytometer for detection of circulating tumor cells. Poster session presented at 56e Voorjaarscongres NVKC 2003, Lunteren, Netherlands.
Tibbe, A.G.J. ; van der Kooi, A. ; de Groot, M.R. ; Vermes, I. / CellTracks cytometer for detection of circulating tumor cells. Poster session presented at 56e Voorjaarscongres NVKC 2003, Lunteren, Netherlands.
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title = "CellTracks cytometer for detection of circulating tumor cells",
abstract = "Introduction: In patients with carcinomas, tumor cells are shed into the circulation. The number of the circulating tumor cells is low and technology is needed that has sufficient sensitivity and specificity to enumerate and characterize these cells. The CellTracks system was developed to provide an immunophenotype, fluorescence wave forms as well as images of immuno-magnetically enriched tumor cells with an occurrence frequency <1 per ml whole blood. Methods: 7.5 ml of whole blood of patients having known breast (n=13) or colorectal (n=12) cancers was immunomagnetically enriched for EpCam positive (epithelial) cells reducing the volume to 320 μl. Fluorescent labels against Cytokeratin and DNA were added. As a negative control a CD45 labelled fluorescent probe against white blood cells was used. This sample was transferred to a measuring chamber, which was inserted in a specially designed strong magnetic field. The magnetic force aligns the cells on a glass slide. These cells were scanned one by one by a multi-laser scanning system and their fluorescence intensities were measured. As a control samples of healthy volunteers were measured in duplicate. To test the immuno-magnetic collection efficiency a known number of fixed SKBR-3 cells were spiked into blood samples. Results: In all patients samples tumor cells were detected. The number of tumor cells found ranged from 1-120 per 7.5 ml of whole blood. For the control samples 0-1 positive cells per 7.5 ml have been detected. The overall immuno-magnetic selection and detection efficiency for SKBR-3 cells was > 70 {\%}. Conclusions: We showed that the novel CellTracks technology has a very high sensitivity and specificity, which is sufficient for the detection of tumor, cells in peripheral blood.",
author = "A.G.J. Tibbe and {van der Kooi}, A. and {de Groot}, M.R. and I. Vermes",
note = "Posterpresentatie tijdens het 56e Congres van de Nederlandse Vereniging voor Klinische Chemie en Laboratoriumgeneeskunde op 16 en 17 april 2003 te Lunteren ; 56e Voorjaarscongres NVKC 2003 ; Conference date: 16-04-2003 Through 17-04-2003",
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Tibbe, AGJ, van der Kooi, A, de Groot, MR & Vermes, I 2002, 'CellTracks cytometer for detection of circulating tumor cells' 56e Voorjaarscongres NVKC 2003, Lunteren, Netherlands, 16/04/03 - 17/04/03, .

CellTracks cytometer for detection of circulating tumor cells. / Tibbe, A.G.J.; van der Kooi, A.; de Groot, M.R.; Vermes, I.

2002. Poster session presented at 56e Voorjaarscongres NVKC 2003, Lunteren, Netherlands.

Research output: Contribution to conferencePosterOther research output

TY - CONF

T1 - CellTracks cytometer for detection of circulating tumor cells

AU - Tibbe, A.G.J.

AU - van der Kooi, A.

AU - de Groot, M.R.

AU - Vermes, I.

N1 - Posterpresentatie tijdens het 56e Congres van de Nederlandse Vereniging voor Klinische Chemie en Laboratoriumgeneeskunde op 16 en 17 april 2003 te Lunteren

PY - 2002/4/16

Y1 - 2002/4/16

N2 - Introduction: In patients with carcinomas, tumor cells are shed into the circulation. The number of the circulating tumor cells is low and technology is needed that has sufficient sensitivity and specificity to enumerate and characterize these cells. The CellTracks system was developed to provide an immunophenotype, fluorescence wave forms as well as images of immuno-magnetically enriched tumor cells with an occurrence frequency <1 per ml whole blood. Methods: 7.5 ml of whole blood of patients having known breast (n=13) or colorectal (n=12) cancers was immunomagnetically enriched for EpCam positive (epithelial) cells reducing the volume to 320 μl. Fluorescent labels against Cytokeratin and DNA were added. As a negative control a CD45 labelled fluorescent probe against white blood cells was used. This sample was transferred to a measuring chamber, which was inserted in a specially designed strong magnetic field. The magnetic force aligns the cells on a glass slide. These cells were scanned one by one by a multi-laser scanning system and their fluorescence intensities were measured. As a control samples of healthy volunteers were measured in duplicate. To test the immuno-magnetic collection efficiency a known number of fixed SKBR-3 cells were spiked into blood samples. Results: In all patients samples tumor cells were detected. The number of tumor cells found ranged from 1-120 per 7.5 ml of whole blood. For the control samples 0-1 positive cells per 7.5 ml have been detected. The overall immuno-magnetic selection and detection efficiency for SKBR-3 cells was > 70 %. Conclusions: We showed that the novel CellTracks technology has a very high sensitivity and specificity, which is sufficient for the detection of tumor, cells in peripheral blood.

AB - Introduction: In patients with carcinomas, tumor cells are shed into the circulation. The number of the circulating tumor cells is low and technology is needed that has sufficient sensitivity and specificity to enumerate and characterize these cells. The CellTracks system was developed to provide an immunophenotype, fluorescence wave forms as well as images of immuno-magnetically enriched tumor cells with an occurrence frequency <1 per ml whole blood. Methods: 7.5 ml of whole blood of patients having known breast (n=13) or colorectal (n=12) cancers was immunomagnetically enriched for EpCam positive (epithelial) cells reducing the volume to 320 μl. Fluorescent labels against Cytokeratin and DNA were added. As a negative control a CD45 labelled fluorescent probe against white blood cells was used. This sample was transferred to a measuring chamber, which was inserted in a specially designed strong magnetic field. The magnetic force aligns the cells on a glass slide. These cells were scanned one by one by a multi-laser scanning system and their fluorescence intensities were measured. As a control samples of healthy volunteers were measured in duplicate. To test the immuno-magnetic collection efficiency a known number of fixed SKBR-3 cells were spiked into blood samples. Results: In all patients samples tumor cells were detected. The number of tumor cells found ranged from 1-120 per 7.5 ml of whole blood. For the control samples 0-1 positive cells per 7.5 ml have been detected. The overall immuno-magnetic selection and detection efficiency for SKBR-3 cells was > 70 %. Conclusions: We showed that the novel CellTracks technology has a very high sensitivity and specificity, which is sufficient for the detection of tumor, cells in peripheral blood.

M3 - Poster

ER -

Tibbe AGJ, van der Kooi A, de Groot MR, Vermes I. CellTracks cytometer for detection of circulating tumor cells. 2002. Poster session presented at 56e Voorjaarscongres NVKC 2003, Lunteren, Netherlands.