@inproceedings{0582940976a34fb8a10c01b3a72f1027,
title = "Changes in human milk lipid composition and conformational state during a single breastfeed",
abstract = "Fat in human milk forms the main energy source for infants and is the most variable component in terms of concentration and composition. Knowledge on changes in human milk lipid composition and conformational state during a single breastfeed contributes to an in-depth understanding of lipid synthesis in the mammary gland. Therefore, the objective of this study was to evaluate the differences in fatty acid length, degree of unsaturation (lipid composition) and lipid phase (lipid conformational state) of milk released at different stages during a breastfeed (fore-, bulk- and hindmilk). A total of 30 samples from 10 lactating subjects were investigated using confocal Raman spectroscopy. No significant differences in lipid composition were observed between fore-, bulk- and hindmilk samples, which is consistent with literature. A new finding from this study is that the lipid conformational state in human fore-, bulk- and hindmilk was significantly different at room temperature. The lipid phase of foremilk was almost crystalline and the lipid phase of hindmilk was almost liquid. Based on this observation, we hypothesize that lipid synthesis in the mammary gland changes during a single breastfeed.",
keywords = "2022 OA procedure",
author = "{de Wolf}, {Johanna R.} and Anki Lenferink and Lenferink, {Aufried T.M.} and Cees Otto and Nienke Bosschaart",
note = "Publisher Copyright: {\textcopyright}2021TheAuthor(s); European Conferences on Biomedical Optics, ECBO 2021, ECBO 2021 ; Conference date: 20-06-2021 Through 24-06-2021",
year = "2021",
doi = "10.1117/12.2615815",
language = "English",
series = "SPIE Conference Proceedings",
publisher = "The Optical Society",
editor = "Emmanuel Beaurepaire and Adela Ben-Yakar and YongKeun Park",
booktitle = "European Conferences on Biomedical Optics, ECBO 2021",
address = "United States",
}