CHAP is a newly identified Z-disc protein essential for heart and skeletal muscle function

Abdelaziz Beqqali, Jantine Monshouwer-Kloots, Rui Monteiro, Maaike Welling, Jeroen Bakkers, Elisabeth Ehler, Arie Verkleij, Christine Mummery, Robert Passier*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

31 Citations (Scopus)
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Abstract

In recent years, the perception of Z-disc function has changed from a passive anchor for myofilaments that allows transmission of force, to a dynamic multicomplex structure, capable of sensing and transducing extracellular signals. Here, we describe a new Z-disc protein, which we named CHAP (cytoskeletal heart-enriched actin-associated protein), expressed in differentiating heart and skeletal muscle in vitro and in vivo. Interestingly, in addition to its sarcomeric localization, CHAP was also able to translocate to the nucleus. CHAP was associated with filamentous actin in the cytoplasm and the nucleus when expressed ectopically in vitro, but in rat neonatal cardiomyocytes, CHAP disrupted the subcellular localization of a-actinin, another Z-disc protein. More importantly, knockdown of CHAP in zebrafish resulted in aberrant cardiac and skeletal muscle development and function. These findings suggest that CHAP is a critical component of the sarcomere with an important role in muscle development.

Original languageEnglish
Pages (from-to)1141-1150
Number of pages10
JournalJournal of cell science
Volume123
Issue number7
DOIs
Publication statusPublished - 1 Apr 2010
Externally publishedYes

Fingerprint

Actins
Myocardium
Skeletal Muscle
Proteins
Muscle Development
Actinin
Sarcomeres
Myofibrils
Cardiac Myocytes
plasma protein Z
Cytoplasm
In Vitro Techniques

Keywords

  • CHAP
  • Heart development
  • Muscle
  • Sarcomere
  • Synpo2l
  • Z-disc

Cite this

Beqqali, A., Monshouwer-Kloots, J., Monteiro, R., Welling, M., Bakkers, J., Ehler, E., ... Passier, R. (2010). CHAP is a newly identified Z-disc protein essential for heart and skeletal muscle function. Journal of cell science, 123(7), 1141-1150. https://doi.org/10.1242/jcs.063859
Beqqali, Abdelaziz ; Monshouwer-Kloots, Jantine ; Monteiro, Rui ; Welling, Maaike ; Bakkers, Jeroen ; Ehler, Elisabeth ; Verkleij, Arie ; Mummery, Christine ; Passier, Robert. / CHAP is a newly identified Z-disc protein essential for heart and skeletal muscle function. In: Journal of cell science. 2010 ; Vol. 123, No. 7. pp. 1141-1150.
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Beqqali, A, Monshouwer-Kloots, J, Monteiro, R, Welling, M, Bakkers, J, Ehler, E, Verkleij, A, Mummery, C & Passier, R 2010, 'CHAP is a newly identified Z-disc protein essential for heart and skeletal muscle function', Journal of cell science, vol. 123, no. 7, pp. 1141-1150. https://doi.org/10.1242/jcs.063859

CHAP is a newly identified Z-disc protein essential for heart and skeletal muscle function. / Beqqali, Abdelaziz; Monshouwer-Kloots, Jantine; Monteiro, Rui; Welling, Maaike; Bakkers, Jeroen; Ehler, Elisabeth; Verkleij, Arie; Mummery, Christine; Passier, Robert.

In: Journal of cell science, Vol. 123, No. 7, 01.04.2010, p. 1141-1150.

Research output: Contribution to journalArticleAcademicpeer-review

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Beqqali A, Monshouwer-Kloots J, Monteiro R, Welling M, Bakkers J, Ehler E et al. CHAP is a newly identified Z-disc protein essential for heart and skeletal muscle function. Journal of cell science. 2010 Apr 1;123(7):1141-1150. https://doi.org/10.1242/jcs.063859