Abstract
In this research, we aim to characterize extracellular vesicles(EVs) with Confocal Raman spectroscopy to reveal relevant spectral lines that signify differences between EVs derived from different cell lines.
In the first stage we performed confocal Raman measurements on various EV samples. For these experiments, a 50 µl droplet of EVs was placed in a hollow area on a slide which was covered with a thin glass disk. Both the slide and the disk consist of borosilicate glass. A well-defined laser beam was focused on the sample inside the cavity. Clusters of EVs were trapped in the focal volume by the single-beam gradient force.
In parallel we are trying to increase the signal to noise ratio (SNR) by increasing the EV density and to collect signal only from desired EVs. We developed a PDMS microchannel device to attract specific EVs and enrich its concentration at the surface area of the microchannel device.
In the first stage we performed confocal Raman measurements on various EV samples. For these experiments, a 50 µl droplet of EVs was placed in a hollow area on a slide which was covered with a thin glass disk. Both the slide and the disk consist of borosilicate glass. A well-defined laser beam was focused on the sample inside the cavity. Clusters of EVs were trapped in the focal volume by the single-beam gradient force.
In parallel we are trying to increase the signal to noise ratio (SNR) by increasing the EV density and to collect signal only from desired EVs. We developed a PDMS microchannel device to attract specific EVs and enrich its concentration at the surface area of the microchannel device.
Original language | English |
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Publication status | Published - 17 Jan 2017 |
Event | Physics@Veldhoven 2017 - De Koningshof, Veldhoven, Netherlands Duration: 17 Jan 2017 → 18 Jan 2017 |
Conference
Conference | Physics@Veldhoven 2017 |
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Country/Territory | Netherlands |
City | Veldhoven |
Period | 17/01/17 → 18/01/17 |
Keywords
- Raman microspectroscopy
- Laser physics
- Principal component analysis
- Extracellular vesicles