Characterization of Extracellular Vesicles using Raman Spectroscopy

Research output: Contribution to conferencePosterOther research output

Abstract

In this research, we aim to characterize extracellular vesicles(EVs) with Confocal Raman spectroscopy to reveal relevant spectral lines that signify differences between EVs derived from different cell lines.
In the first stage we performed confocal Raman measurements on various EV samples. For these experiments, a 50 µl droplet of EVs was placed in a hollow area on a slide which was covered with a thin glass disk. Both the slide and the disk consist of borosilicate glass. A well-defined laser beam was focused on the sample inside the cavity. Clusters of EVs were trapped in the focal volume by the single-beam gradient force.
In parallel we are trying to increase the signal to noise ratio (SNR) by increasing the EV density and to collect signal only from desired EVs. We developed a PDMS microchannel device to attract specific EVs and enrich its concentration at the surface area of the microchannel device.
Original languageEnglish
Publication statusPublished - 17 Jan 2017
EventPhysics@Veldhoven 2017 - De Koningshof, Veldhoven, Netherlands
Duration: 17 Jan 201718 Jan 2017

Conference

ConferencePhysics@Veldhoven 2017
CountryNetherlands
CityVeldhoven
Period17/01/1718/01/17

Fingerprint

microchannels
chutes
Raman spectroscopy
borosilicate glass
cultured cells
line spectra
hollow
signal to noise ratios
laser beams
gradients
cavities
glass

Keywords

  • Raman microspectroscopy
  • Laser physics
  • Principal component analysis
  • Extracellular vesicles

Cite this

Lee, W., Nanou, A., Terstappen, L. W. M. M., Rho, H. S., le Gac, S., & Offerhaus, H. L. (2017). Characterization of Extracellular Vesicles using Raman Spectroscopy. Poster session presented at Physics@Veldhoven 2017, Veldhoven, Netherlands.
@conference{a867ee96cff84249a35a3465e2853bbd,
title = "Characterization of Extracellular Vesicles using Raman Spectroscopy",
abstract = "In this research, we aim to characterize extracellular vesicles(EVs) with Confocal Raman spectroscopy to reveal relevant spectral lines that signify differences between EVs derived from different cell lines. In the first stage we performed confocal Raman measurements on various EV samples. For these experiments, a 50 µl droplet of EVs was placed in a hollow area on a slide which was covered with a thin glass disk. Both the slide and the disk consist of borosilicate glass. A well-defined laser beam was focused on the sample inside the cavity. Clusters of EVs were trapped in the focal volume by the single-beam gradient force. In parallel we are trying to increase the signal to noise ratio (SNR) by increasing the EV density and to collect signal only from desired EVs. We developed a PDMS microchannel device to attract specific EVs and enrich its concentration at the surface area of the microchannel device.",
keywords = "Raman microspectroscopy, Laser physics, Principal component analysis, Extracellular vesicles",
author = "Wooje Lee and Afroditi Nanou and Terstappen, {Leonardus Wendelinus Mathias Marie} and Rho, {Hoon Suk} and {le Gac}, Severine and Offerhaus, {Herman L.}",
year = "2017",
month = "1",
day = "17",
language = "English",
note = "Physics@Veldhoven 2017 ; Conference date: 17-01-2017 Through 18-01-2017",

}

Lee, W, Nanou, A, Terstappen, LWMM, Rho, HS, le Gac, S & Offerhaus, HL 2017, 'Characterization of Extracellular Vesicles using Raman Spectroscopy' Physics@Veldhoven 2017, Veldhoven, Netherlands, 17/01/17 - 18/01/17, .

Characterization of Extracellular Vesicles using Raman Spectroscopy. / Lee, Wooje ; Nanou, Afroditi ; Terstappen, Leonardus Wendelinus Mathias Marie; Rho, Hoon Suk; le Gac, Severine ; Offerhaus, Herman L.

2017. Poster session presented at Physics@Veldhoven 2017, Veldhoven, Netherlands.

Research output: Contribution to conferencePosterOther research output

TY - CONF

T1 - Characterization of Extracellular Vesicles using Raman Spectroscopy

AU - Lee, Wooje

AU - Nanou, Afroditi

AU - Terstappen, Leonardus Wendelinus Mathias Marie

AU - Rho, Hoon Suk

AU - le Gac, Severine

AU - Offerhaus, Herman L.

PY - 2017/1/17

Y1 - 2017/1/17

N2 - In this research, we aim to characterize extracellular vesicles(EVs) with Confocal Raman spectroscopy to reveal relevant spectral lines that signify differences between EVs derived from different cell lines. In the first stage we performed confocal Raman measurements on various EV samples. For these experiments, a 50 µl droplet of EVs was placed in a hollow area on a slide which was covered with a thin glass disk. Both the slide and the disk consist of borosilicate glass. A well-defined laser beam was focused on the sample inside the cavity. Clusters of EVs were trapped in the focal volume by the single-beam gradient force. In parallel we are trying to increase the signal to noise ratio (SNR) by increasing the EV density and to collect signal only from desired EVs. We developed a PDMS microchannel device to attract specific EVs and enrich its concentration at the surface area of the microchannel device.

AB - In this research, we aim to characterize extracellular vesicles(EVs) with Confocal Raman spectroscopy to reveal relevant spectral lines that signify differences between EVs derived from different cell lines. In the first stage we performed confocal Raman measurements on various EV samples. For these experiments, a 50 µl droplet of EVs was placed in a hollow area on a slide which was covered with a thin glass disk. Both the slide and the disk consist of borosilicate glass. A well-defined laser beam was focused on the sample inside the cavity. Clusters of EVs were trapped in the focal volume by the single-beam gradient force. In parallel we are trying to increase the signal to noise ratio (SNR) by increasing the EV density and to collect signal only from desired EVs. We developed a PDMS microchannel device to attract specific EVs and enrich its concentration at the surface area of the microchannel device.

KW - Raman microspectroscopy

KW - Laser physics

KW - Principal component analysis

KW - Extracellular vesicles

M3 - Poster

ER -

Lee W, Nanou A, Terstappen LWMM, Rho HS, le Gac S, Offerhaus HL. Characterization of Extracellular Vesicles using Raman Spectroscopy. 2017. Poster session presented at Physics@Veldhoven 2017, Veldhoven, Netherlands.