Characterization of Extracellular Vesicles using Raman Spectroscopy

Research output: Contribution to conferencePoster

Abstract

In this research, we aim to characterize extracellular vesicles(EVs) with Confocal Raman spectroscopy to reveal relevant spectral lines that signify differences between EVs derived from different cell lines.
In the first stage we performed confocal Raman measurements on various EV samples. For these experiments, a 50 µl droplet of EVs was placed in a hollow area on a slide which was covered with a thin glass disk. Both the slide and the disk consist of borosilicate glass. A well-defined laser beam was focused on the sample inside the cavity. Clusters of EVs were trapped in the focal volume by the single-beam gradient force.
In parallel we are trying to increase the signal to noise ratio (SNR) by increasing the EV density and to collect signal only from desired EVs. We developed a PDMS microchannel device to attract specific EVs and enrich its concentration at the surface area of the microchannel device.
Original languageEnglish
Publication statusPublished - 17 Jan 2017
EventPhysics@Veldhoven 2017 - De Koningshof, Veldhoven, Netherlands
Duration: 17 Jan 201718 Jan 2017

Conference

ConferencePhysics@Veldhoven 2017
CountryNetherlands
CityVeldhoven
Period17/01/1718/01/17

Keywords

  • Raman microspectroscopy
  • Laser physics
  • Principal component analysis
  • Extracellular vesicles

Fingerprint Dive into the research topics of 'Characterization of Extracellular Vesicles using Raman Spectroscopy'. Together they form a unique fingerprint.

  • Cite this

    Lee, W., Nanou, A., Terstappen, L. W. M. M., Rho, H. S., le Gac, S., & Offerhaus, H. L. (2017). Characterization of Extracellular Vesicles using Raman Spectroscopy. Poster session presented at Physics@Veldhoven 2017, Veldhoven, Netherlands.